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Gold nano bipyramid array substrate, preparation method and application thereof

A gold nanometer and cone array technology is applied in the field of gold nanometer double cone array substrate and its preparation, which can solve the problems that the sensitivity and reproducibility of the SERS substrate cannot meet the application requirements, and it is difficult to detect trace amounts of ciprofloxacin. High accuracy, high sensitivity and uniform distribution of hot spots

Pending Publication Date: 2021-12-10
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sensitivity and reproducibility of the existing SERS substrates cannot meet the application requirements, and it is difficult to apply to the detection of trace ciprofloxacin

Method used

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  • Gold nano bipyramid array substrate, preparation method and application thereof
  • Gold nano bipyramid array substrate, preparation method and application thereof
  • Gold nano bipyramid array substrate, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0041] Au NBPs were prepared according to the heat-induced seed growth method reported in the literature, and the specific steps were as follows:

[0042] (1) Synthesis of gold species: under vigorous stirring, freshly prepared NaBH 4 The ice solution (25mM, 0.75mL) was quickly added to the solution containing 50mM CTAC, 5mM citric acid, 2.5mM HAuCl 4 30mL of the mixed solution, and then stirred for 2min. The color of the solution will turn brown, and then put it into an 80°C oil bath for 90 minutes, and the color will turn red. The gold seed solution was stored at room temperature for use in subsequent experiments.

[0043] (2) Synthesis of Au NBPs by seed growth method: 8 mL of gold seed solution was added to the prepared HAuCl-containing 4 (10mM, 10mL), AgNO 3 (10mM, 2mL), HCl (1.0M, 4mL), Vc (0.1M, 1.6mL) and CTAB (0.1M, 200mL) growth solution, stirred slowly for 2min to mix well. Finally, the above mixed solution was placed in a water bath at 30 °C for 3 h. During t...

Embodiment 1

[0047] (1) Mix 1mM PEG-SH aqueous solution and 1mM C 16 -SH aqueous solution mixed in different volume ratios: 100% PEG-SH (A), 50% PEG-SH: 50% C 16 -SH(B), 30%PEG-SH: 70%C 16 -SH(C), 100%C 16 -SH(D), take 50 μL of the Au NBPs solution in Preparation Example 1, slowly add PEG-SH and C 16 -SH mixed solution, shaking reaction for 8h for ligand exchange. Afterwards, centrifuge to remove excess ligand mixed solution, redisperse the precipitate in a water-isopropanol solution with a volume ratio of 2:1, repeat centrifugation and washing three times, and finally disperse in pure water for later use to obtain PEG- SH and C 16 -SH-modified Au NBPs solution. The effect of ligand exchange on the surface of Au NBPs was verified using a nanoparticle size potentiometer (Zetasize Nano ZS 90, Malvern Instruments Ltd., UK).

[0048] (2) With n-decane as the oil phase, take 10 μL of PEG-SH and C in step (1) 16 The -SH-modified AuNBP solution was added to 500 μL of n-decane, mixed by man...

Embodiment 2

[0054] (1) Disperse the Au NBPs precipitate prepared in Example 1 in water, centrifuge to remove excess CTAB on the surface, and then redisperse with water / isopropanol (2:1) to prepare an Au NBPs solution with a concentration of 10 nM. Stirring conditions, slowly drop 12.5, 25, 50, 100, 200 μL of PEG-SH and C with a volume ratio of 50:50, respectively 16 -SH mixed solution, shaking reaction for 8h for ligand exchange. After that, centrifuge to remove excess ligand mixed solution, redisperse the precipitate in a water-isopropanol solution with a volume ratio of 2:1, repeat centrifugation and washing three times, and finally disperse in pure water for later use to obtain different volumes Ligand-modified AuNBPs solution.

[0055] (2) Using n-decane as the oil phase, take 10 μL of ligand-modified Au NBPs solutions of different volumes in step (1) and add them to 500 μL of n-decane, shake and mix manually, and sonicate at room temperature for 10 minutes, pass through the oil- Th...

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Abstract

The invention discloses a gold nano bipyramid array substrate, a preparation method and application thereof. The gold nano bipyramid array substrate comprises a substrate and a film loaded on the surface of the substrate and formed by assembling gold nano bipyramids, wherein the surfaces of the gold nano bipyramids are modified with hydrophilic ligands and hydrophobic ligands, and the gold nano double-cone array substrate is strong in Raman signal, high in selectivity to ciprofloxacin, capable of detecting trace ciprofloxacin in a to-be-detected sample and good in application prospect.

Description

technical field [0001] The invention relates to the technical field of surface-enhanced Raman scattering substrates, in particular to a gold nano bicone array substrate and its preparation method and application. Background technique [0002] Ciprofloxacin (CIP) belongs to the third generation of quinolone antibacterial drugs. It has strong antibacterial activity, low toxicity and is not easy to produce drug resistance. Therefore, it has become one of the widely used antibiotics. Currently, methods for qualitative and quantitative detection of CIP include UV-visible spectrophotometry, fluorescence, capillary electrophoresis, microemulsion electrokinetic chromatography, high performance liquid chromatography and their combined techniques. Although these methods have their own advantages, However, there are certain limitations, such as high instrument cost, long detection time, and complicated pretreatment of samples. [0003] Surface-enhanced Raman scattering (SERS), as a ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/65
CPCG01N21/01G01N21/658
Inventor 姚媛媛郭隆华王悦靓陈丽芬林露林丙永
Owner JIAXING UNIV
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