Simple in-vitro dynamic digestion simulation device and use method

Abstract

The invention discloses a simple in-vitro dynamic digestion simulation device and a use method. The in-vitro simulation digestion device comprises a simulation oral cavity part, a simulation stomach part and a simulation small intestine part which are arranged in a constant-temperature box and are connected in sequence; a first bracket, a second bracket and a third bracket are arranged in the constant-temperature box; the simulation oral cavity part is an oral cavity reaction bottle, and the oral cavity reaction bottle is hung below the first bracket; and the simulation stomach part is composed of a gastric juice storage bottle and a gastric reaction bottle, wherein the gastric reaction bottle is hung below the second bracket, and the gastric juice storage bottle is connected below the first bracket. The device is simple in structure and low in implementation cost, the gastrointestinal simulation digestion stage is not static and unchanged, the device is close to the processes of gastric secretion, gastric emptying, biliary secretion, pancreatic juice secretion and the like of the human body, and the real digestion process of the human body can be better simulated. All the parts can be split and combined, the digestion condition of one or more parts is researched by combining the actual condition, and convenience is provided for experiments.

Description

technical field [0001] The invention belongs to the technical field of in vitro dynamic simulated digestion, and in particular relates to an in vitro dynamic simulated digestion device and its use method Background technique [0002] The prevalence of diet-related diseases has been increasing worldwide over the past few decades. Researchers have conducted a large number of studies linking diet and health in different populations. However, understanding the physiological response to specific foods requires a more detailed understanding of the complex digestive processes within the human digestive tract. [0003] At present, the methods commonly used to study the digestion process of food and drugs in the human body mainly include in vivo models and in vitro models. In vivo models include human experiments and animal experiments, but in vivo experiments are expensive, time-consuming, and have too many influencing factors. Due to various problems such as low reproducibility a...

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Problems solved by technology

However, static digestion methods also have many limitations, such as the inability to reproduce the morphology of the digestive tract, peristaltic contractions, and some dynamic processes such as secretion of digestive juices, gastric emptying, etc.

Moreover, although different intestinal segments have different physicochemical properties and microbial content, intestinal digestion is only used as a stage in the static digestion model

[0004] Therefore, how to get closer to the real digestive environment of the human body on the basis of simple device and easy operation is an important research problem, such as "a dynamic digestion model of food allergens and in vitro simulation evaluation method" ", the dynamic digestion model can preset the digestion conditions of each stage, there is no need to stop midway during the digestion process, and the whole process is automatically and continuously digested, but the model device is relatively complicated, requiring 8 constant flow pumps to be controlled at the same time, and gastric emptying is not considered. Digestive juice secretion and other real digestive processes

Mulet-Cabero et al. developed a low-cost semi-dynamic gastric digestion model and described it in detail (Food Hydrocolloids, 2017, 67:63-73), which can simulate the main gastric dynamic process, Including the gradual acidification of the gastric environment and the gastric emptying process, but the disadvantage is that although the gastric emptying process is simulated, it needs to be done manually, and only the semi-dynamic optimization of the gastric digestion stage is performed, and the intestinal digestion stage is still static

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