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Method for obtaining soil animal activity biosensor for farmland soil health diagnosis

A technology for farmland soil animals and biosensors, which is applied in the agricultural field and can solve the problems of authenticity, accuracy, poor reliability, authenticity, accuracy, sensitivity and reliability, adaptability, sensitivity and tolerance. , to achieve strong adaptability and highlight the effect of authenticity

Pending Publication Date: 2021-12-03
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) Existing biosensors use cultured cells, microorganisms, and tissues as sensitive materials, which are not in situ soil animals in farmland. The adaptability, sensitivity and tolerance of existing sensitive materials to field farmland soil The accuracy is poor, and the authenticity, accuracy, sensitivity and reliability of the detection are poor;
[0005] (2) The existing biosensors capture the reaction between the target object and the sensitive material through various physical and chemical transducers, and cannot monitor the complex ecological process between the soil animal itself and the farmland soil environment in real time, all-weather, and in a full cycle;
[0006] (3) Existing biosensors convert the degree of reaction into electrical signals, and calculate the measured value according to the electrical signals. This is a process of indirect speculation and monitoring. The authenticity, accuracy and reliability of the detection are relatively high Difference

Method used

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  • Method for obtaining soil animal activity biosensor for farmland soil health diagnosis
  • Method for obtaining soil animal activity biosensor for farmland soil health diagnosis
  • Method for obtaining soil animal activity biosensor for farmland soil health diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] A method for obtaining a soil animal activity biosensor for farmland soil health diagnosis, comprising the following steps:

[0085] Step 1: Screening of green eco-buoys for farmland soil animal activity biosensors:

[0086] ①. Collect soil animals in the wild farmland, and bring the collected live soil animals back to the laboratory:

[0087] Collect farmland soil animals in the wild farmland, put the collected soil animal samples into specimen bottles or specimen boxes, and isolate predators from prey and competitors; then cover them to prevent soil animals from escaping, and use tools to Make some small holes on the cover to ensure that there is enough air in the specimen bottle or specimen box. The diameter of the small holes is less than 2mm to prevent the soil animals inside from escaping; then, put in-situ soil particles and Littered leaves; use the soil column method to collect a soil column or soil cube with a depth of 15cm in the field farmland, put it into a...

Embodiment 2

[0138] Such as Figure 1-Figure 3 Shown, a kind of farmland soil animal activity biosensor field experiment device comprises ecological box and power supply device; Described ecological box is made up of upper floor ecological box 4, middle floor ecological box 5 and lower floor ecological box 6, described upper floor ecological box 4, The middle-level ecological box 5 and the lower-level ecological box 6 are both up and down through structures, and the upper-level ecological box 4, the middle-level ecological box 5 and the lower-level ecological box 6 are detachably connected by the connecting ring 7 from top to bottom;

[0139] The power supply device includes a solar panel 10, a support rod 12, an electrical conduit 15 and a backup power supply 9, the electrical conduit 15 is composed of a vertical section and a horizontal section, and the bottom end of the vertical section passes through an elbow 16 and one end of the horizontal section Connect, the top of the vertical sec...

Embodiment 3

[0191] Such as Figure 4 Shown: 1. Field real-time automatic monitoring process: use the high-definition camera, sound recorder, etc. inside the soil animal activity biosensor field experiment ecological box to automatically monitor the relationship between the selected soil animal green ecological buoys (ie soil animals) and the soil environment in real time. Interaction process, so as to obtain high-definition, real-time data of the interaction between the green ecological buoy of soil animals and the soil environment.

[0192] 2. Real-time automatic data transmission process: Using modern technologies and methods such as 5G network, Internet +, cloud platform, etc., the high-definition and real-time data of the interaction between the soil animal green ecological buoy and the soil environment obtained above are automatically transmitted to the experiment in real time. Office data management center.

[0193] 3. Data real-time storage, management and analysis process: Based ...

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Abstract

The invention discloses a method for obtaining a soil animal activity biosensor for farmland soil health diagnosis. The method includes the following steps: 1, screening green ecological buoys of the farmland soil animal activity biosensor; and 2, carrying out field domestication on the farmland soil animal activity biosensor green ecological buoy based on a farmland soil animal activity biosensor field experiment device. The soil animal activity biosensor obtained by the method takes in-situ soil animals in a farmland as green ecological buoys, and is a complex and natural ecological process capable of diagnosing the health condition of farmland soil; the method has extremely high adaptability, sensitivity and tolerance to field farmland soil, and has outstanding authenticity, accuracy, sensitivity and reliability when reflecting the health condition of the soil; the complex ecological process between the soil animals and the farmland soil environment is automatically monitored in real time in an all-weather and full-period mode through automatic monitoring instruments and equipment.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a method for obtaining a soil animal activity biosensor for farmland soil health diagnosis. Background technique [0002] Biosensors are immobilized biological components (such as enzymes, proteins, DNA antibodies, antigens) or organisms themselves (such as cells, microorganisms, tissues, etc.) as sensitive materials, combined with appropriate chemical converters for rapid monitoring Novel devices for physics, chemistry, and biomass. Biosensors capture the reaction between the target and sensitive materials through various physical and chemical transducers, and then convert the degree of reaction into electrical signals, and calculate the measured value based on the electrical signals. Biosensors have developed rapidly in recent years and have been used in medicine, fermentation production, food industry, environmental monitoring and other fields, and have played an import...

Claims

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Application Information

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IPC IPC(8): G01N33/24A01K15/00
CPCG01N33/24A01K15/00Y02A40/70
Inventor 高梅香李加林朱家祺
Owner NINGBO UNIV
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