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Method for screening prostate cancer PSA diagnosis gray region serum marker

A technology of serum markers and diagnostic markers, applied in the field of biomedicine, can solve the problems of regular monitoring of patients, poor specificity of prostate cancer, discomfort and complications, etc., to avoid denaturation and inactivation, prevent protein aggregation, and maintain biological active effect

Inactive Publication Date: 2021-12-03
徐蓓 +3
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its invasive nature, cumbersome procedure, and attendant severe discomfort and complications preclude its use for routine monitoring of patients
In addition, due to the poor specificity of serum prostate-specific antigen (PSA) results for the detection of prostate cancer, many individuals without prostate cancer also undergo transrectal ultrasound-guided prostate biopsy in order to rule out that the individual does not have prostate cancer. lead to potential complications

Method used

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  • Method for screening prostate cancer PSA diagnosis gray region serum marker
  • Method for screening prostate cancer PSA diagnosis gray region serum marker
  • Method for screening prostate cancer PSA diagnosis gray region serum marker

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0062] Embodiments of the present invention will be further described below in conjunction with the accompanying drawings.

[0063] like Figure 9 Shown: a method for screening serum markers in the gray zone of prostate cancer PSA diagnosis, mainly based on the detection of non-targeted metabolomics, including the following steps:

[0064] S10, sample collection: the venous blood of several PCa patients, BPH patients and healthy controls who have not yet started tumor treatment, and the venous blood is centrifuged, and stored at low temperature, corresponding to several PCa patients, BPH patients and healthy controls Serum samples from control subjects;

[0065] S20, pretreatment of metabolomics: add positive ion internal standard and negative ion internal standard to the serum sample, then add methanol-acetonitrile solution of appropriate concentration and volume, undergo vortex, water bath ultrasonication, incubation and centrifugation in sequence, and then take After the ...

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Abstract

The invention discloses a method for screening a prostate cancer PSA diagnosis gray region serum marker. The method comprises the following steps of S10, collecting a sample; S20, carrying out metabonomics pretreatment; S30, arranging a to-be-tested sample analysis operation sequence; S40, carrying out a non-targeted metabonomics experiment; S50, qualitatively detecting the original data of metabolites; S60, screening differential metabolites among the samples to be detected; S70, carrying out metabolic pathway analysis; S80, screening serum metabolites, and determining candidate diagnostic markers; and S90, determining a diagnostic marker. The invention provides a biomarker which is efficient, minimally invasive and capable of accurately screening prostatic cancer and distinguishing prostatic hyperplasia, so that the procedure for detecting the prostatic cancer is simplified, and complications caused by rectal ultrasound guided prostatic biopsy diagnosis are avoided.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for screening gray zone serum markers in PSA diagnosis of prostate cancer. Background technique [0002] Prostate cancer (PCa) is currently the second most common male malignancy worldwide, with more than 1 million new cases and more than 350,000 deaths. [0003] In the diagnosis and location of prostate cancer, digital rectal examination and serum prostate-specific antigen detection are mainly relied on, followed by transrectal ultrasound-guided prostate biopsy for diagnosis. [0004] Serum prostate-specific antigen (PSA) is an androgen-regulated serine protease produced and secreted by prostate epithelial cells, with a normal level of about 0-1.5 ng / mL. When the serum prostate-specific antigen (PSA) level is elevated (>4ng / mL) alone, it can only predict the possibility of prostate cancer, and benign prostatic hyperplasia (BPH) can also make the serum prostate-s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/32G01N30/34G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/32G01N30/34G01N30/72G01N30/8675G01N2030/324
Inventor 徐蓓陈燕俸家富曾家伟
Owner 徐蓓
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