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Mercury ion ratio fluorescence detection method with controllable sensitivity

A technology of ratiometric fluorescence and detection method, which is applied in the field of fluorescence detection, and can solve the problems of variability, inactivation, high requirements for reaction conditions, etc.

Active Publication Date: 2021-12-03
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Biological enzymes refer to proteins with specific catalytic activity, most of which are composed of long chains of amino acids, with high catalytic ability and specificity, but their variability and inactivation require high reaction conditions

Method used

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  • Mercury ion ratio fluorescence detection method with controllable sensitivity
  • Mercury ion ratio fluorescence detection method with controllable sensitivity
  • Mercury ion ratio fluorescence detection method with controllable sensitivity

Examples

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Embodiment 1

[0042] (1) After adding 240 μL of ultrapure water to several 50 μL PtNPs solutions, add 70 μL of Hg with a concentration of 0.02, 0.2, 0.5, 0.8, 1.2, 1.5, 1.8, 2.2, 2.5, 2.8, and 3 μM respectively. 2+ Standard solution, incubate at room temperature for 5 min.

[0043] (2) Add 120 μL of OPD aqueous solution and 120 μL of H to the mixed solution prepared in step (1). 2 o 2 Aqueous solution (OPD and H 2 o 2 The concentration is 20mM), and after incubating the mixture solution at room temperature for 60min, PtNPs catalyzed the oxidation of OPD to generate luminescent DAP;

[0044] (3) Add 100 μL of SiQDs aqueous solution to the mixed solution prepared in step (2); use a fluorescence spectrophotometer to detect the fluorescence intensity of the solution at 414nm and 576nm respectively at room temperature, and the resulting spectrogram is as follows Figure 5 Shown in A; Simultaneously obtain the fluorescence intensity I 576 / I 419 Ratio and Hg 2+ The concentration standard c...

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Abstract

The invention belongs to the technical field of fluorescence detection, and discloses a mercury ion ratio fluorescence detection method with controllable sensitivity. According to the invention, the platinum nanoparticles can catalytically oxidize 2, 3-diaminophenazine generated by o-phenylenediamine, a fluorescence resonance energy transfer effect exists between DAP and the silicon quantum dots, the dual-emission fluorescent probe is obtained, and mercury ion detection is realized by utilizing the characteristic that mercury ions have an inhibition effect on PtNPs activity. In addition, the mercury ion detection sensitivity can be adjusted by adjusting the amount of OPD, and the OPD amount capable of achieving the optimal sensitivity is selected to achieve sensitive detection of mercury ions in an actual sample. According to the sensitivity-controllable mercury ion ratio fluorescence detection method provided by the invention, the linear range of mercury ion detection is 2.0*10<-8>-3.0*10 <-6> mol.L <-1>, the detection limit is 1.05*10 <-8> mol.L <-1>, and the sensitivity-controllable ratio fluorescence detection method is provided for determining mercury ions in an actual sample.

Description

technical field [0001] The invention belongs to the technical field of fluorescence detection, and in particular relates to a mercury ion ratio fluorescence detection method with controllable sensitivity. Background technique [0002] Mercury (Hg) is listed as one of the most harmful heavy metals. It is difficult to degrade and can accumulate in animals, plants and human bodies, and it has serious damage to the human central nervous system. In order to monitor Hg in environmental water and soil resources 2+ pollution situation, avoid Hg 2+ To prevent poisoning and protect human health, scientists have developed various analytical and detection techniques, such as inductively coupled plasma mass spectrometry, atomic absorption spectroscopy, electrochemical and fluorescence detection methods, etc. Among them, the fluorescence method is widely used in the detection of mercury ions due to its high sensitivity and fast response speed. In recent years, methods that utilize the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/06C09K11/59
CPCG01N21/643C09K11/06C09K11/59C09K2211/1044G01N2021/6432
Inventor 由天艳李文佳刘东李玉叶孟淑云
Owner JIANGSU UNIV
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