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Hypsizigus marmoreus factory solid strain production process

A technology of solid strains and production technology, which is applied in horticulture, mushroom cultivation, botanical equipment and methods, etc., and can solve the problems of unstable strain quality, limitations in the improvement of the vitality of jiji mushroom strains, and difficulty in controlling the production quality of strains, etc. problems, to achieve the effect of improving output quality, ensuring cultivation efficiency, and ensuring production quality

Pending Publication Date: 2021-12-03
韶关市星河生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the existing production of shimeji mushroom, the cultivation effect of liquid strains is not good, and the production quality of the strains is difficult to control, which limits the improvement of the vitality of shiji mushroom strains, and the unstable quality of the produced strains directly affects the quality of the strains. Efficient Production of Mushrooms

Method used

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  • Hypsizigus marmoreus factory solid strain production process
  • Hypsizigus marmoreus factory solid strain production process
  • Hypsizigus marmoreus factory solid strain production process

Examples

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Embodiment 1

[0036] Such as figure 1 As shown, a kind of industrialized solid strain production process of shiji mushroom in a preferred embodiment of the present invention comprises the following steps:

[0037] S100, culturing on flat plates: the prepared culture medium is divided into flat plates, and the mycelium of test tube species with uniform growth is selected, and inoculated and cultivated in a sterile environment.

[0038] In this process, unqualified plate species such as poor growth, uneven growth, and bacterial infection are eliminated.

[0039] As a preferred solution, in step S100, the characteristics of the test-tube species mycelium with uniform growth are as follows:

[0040] The appearance is uniform, the hyphae are white and strong, the growth is neat, the culture medium has no drying shrinkage, and there are no spots, streaks or shadows except the inoculated spots when observed from the reverse side;

[0041] Uniform growth refers to the observation of the growth st...

Embodiment 2

[0095] This embodiment is basically the same as Example 1, the difference is that in step S400, the growth of mycelia in the cultivation bottle is observed, and it is required that the mycelia reach 1.2 cm from the shoulder of the bottle for 5 days, and mycelium germinates at the bottom of the cultivation bottle.

[0096] As a preferred solution, in step S400, observe the growth of the mycelia in the cultivation bottle, and it is required that the mycelia reach 2.8 cm below the bottle shoulder in 11 days, and the feeding height at the bottom of the cultivation bottle reaches 1.6 cm.

[0097]As a preferred solution, in step S400, in the cultivation bottle, the mycelia are required to reach through the wall in 18 days; when the bottle is completely full in 30 days, the local color change begins; in 37 days, 90% of the color change is completed: in 44 days, the bottle wall mycelium is thick and white .

Embodiment 3

[0099] This embodiment is basically the same as Example 1, the difference is that in step S400, the growth of mycelia in the cultivation bottle is observed, and it is required that the mycelia reach 1.5 cm from the shoulder of the bottle in 5 days, and mycelium germinates at the bottom of the cultivation bottle.

[0100] As a preferred solution, in step S400, observe the growth of the mycelium in the cultivation bottle, and it is required that the mycelia reach 3 cm below the shoulder of the bottle in 11 days, and the feeding height at the bottom of the cultivation bottle reaches 1.8 cm.

[0101] As a preferred solution, in step S400, the mycelium is required to reach through the wall in 18 days in the cultivation bottle; the full bottle is reached in 30 days, and the part starts to turn color; 90% of the color change is completed in 38 days; the bottle wall mycelium is thick white in 45 days .

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Abstract

The invention relates to the technical field of edible mushroom production, and discloses a hypsizigus marmoreus factory solid strain production process. The hypsizigus marmoreus factory solid strain production process comprises the following steps that plate strains are cultured, specifically, culture mediums are prepared and subpackaged into a plate, test tube strain hyphae with uniform growth vigor are selected, and inoculated culture is carried out in a sterile environment; shake-flask liquid strain culture is carried out, specifically, shake-flask liquid culture medium are prepared and subpackaged into triangular flasks for being sterilized, the plate strains are inoculated into the triangular flasks under sterile conditions, standing is carried out for one day, and shake cultivation is carried out; strains are cultured in a fermentation tank, specifically, fermentation tank culture media are prepared and sterilized, shake flask strains are inoculated into the fermentation tank, sterile air is introduced for culturing, and sampling and detecting are carried out; and solid strains are produced, specifically, strain bottle culture media are prepared and bottled, the strain liquid in the fermentation tank is inoculated to the material surface of a strain bottle in a sterile environment, culturing is carried out until the bottle is full, and then ripening is carried out. The hypsizigus marmoreus factory solid strain production process can be used for efficiently culturing solid strains of hypsizigus marmoreus, the production effect is excellent, the cultured strains are high in consistency, and the required passage frequency is low.

Description

technical field [0001] The invention relates to the technical field of edible fungus production, in particular to a production process of industrialized solid strains of shimeji mushroom. Background technique [0002] Among a wide variety of edible fungi, the mushroom is famous for its smooth and tender cap, crisp handle, rich nutrition, and delicious taste. The production strains of edible fungi include solid strains and liquid strains, and liquid strains are edible fungus strains in liquid form produced by liquid fermentation technology in a biological fermentation tank with a liquid medium. Compared with solid strains, liquid strains have the advantages of fast seed production, strong vitality, shortened cultivation period, and reduced cost. [0003] In the existing production of shimeji mushroom, the cultivation effect of liquid strains is not good, and the production quality of the strains is difficult to control, which limits the improvement of the vitality of shiji m...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/40A01G18/20
Inventor 陈长青许喜佳黄清华林文英严亮吴景潘康会
Owner 韶关市星河生物科技有限公司
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