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Biomarker for detecting autoantibodies with characteristics of different syndromes of hashimoto thyroiditis and application of biomarker

A technology of Hashimoto's thyroiditis and biomarkers, applied in the fields of serum antibody group and immunology technology

Pending Publication Date: 2021-11-30
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no use of omics technology to study the syndrome characteristics and molecular pathogenesis of HT and the molecular mechanism of TCM syndrome differentiation and treatment of HT.

Method used

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  • Biomarker for detecting autoantibodies with characteristics of different syndromes of hashimoto thyroiditis and application of biomarker
  • Biomarker for detecting autoantibodies with characteristics of different syndromes of hashimoto thyroiditis and application of biomarker
  • Biomarker for detecting autoantibodies with characteristics of different syndromes of hashimoto thyroiditis and application of biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Screening of autoantibodies characteristic of different syndromes of Hashimoto's thyroiditis by protein chip

[0042] 1. Source of serum samples

[0043] From January 2017 to December 2019, venous serum samples were collected from the First Affiliated Hospital, the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, and the First Affiliated Hospital of Zhengzhou University from outpatient and inpatient newly diagnosed patients with untreated Hashimoto's thyroiditis. Fifteen patients with different syndromes of Hashimoto’s thyroiditis (5 cases each with liver depression and qi stagnation syndrome-normal thyroid function, yin deficiency and fire hyperactivity syndrome-hyperthyroidism, spleen and kidney yang deficiency syndrome-hypothyroidism) were selected as HuProt. TM There was no statistical difference in the gender and age of the samples detected by the proteome chip in each group.

[0044] The venous serum samples of 5 normal (h...

Embodiment 2

[0094] Example 2. Indirect ELISA experiments verify the screened autoantibodies related to the characteristics of different syndromes of Hashimoto's thyroiditis

[0095] Select relevant autoantibodies TNRC6C, MAP9, and IRF9 in the liver-qi stagnation syndrome group to be verified; relevant autoantibodies PLEKHO2 and NPM3 in the Yin-deficiency fire-excessive syndrome group; , through the indirect ELISA experiment to detect the content of the above-mentioned related autoantibodies in a large sample, and verify the specificity and sensitivity of the related autoantibodies as biomarkers for detecting the characteristics of different syndromes of Hashimoto's thyroiditis.

[0096] 2.1 Source of serum samples

[0097] The required sample size was estimated using PASS 15.0 software. According to the needs of this study, the sample size of the Hashimoto’s thyroiditis patient group is twice that of the normal control group, according to α=0.05, 1-β=0.90, the lowest syndrome type p1 of ...

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Abstract

The invention discloses a biomarker for detecting autoantibodies with characteristics of different syndromes of hashimoto thyroiditis and application of the biomarker. The biomarker is at least one of related autoantibodies of MAP9, IRF9, PLEKHO2, NPM3, DR1, KDM1A, PCGF2, GPCPD1 and IGBP1. The biomarkers for detecting the characteristic autoantibody of the hashimoto thyroiditis liver qi stagnation syndrome are two related autoantibodies, namely MAP9 and IRF9; the biomarkers for detecting the characteristic autoantibody of the syndrome of fire excess from yin deficiency of hashimoto thyroiditis are two related autoantibodies, namely PLEKHO2 and NPM3; the biomarkers for detecting the characteristic autoantibodies of the spleen-kidney yang deficiency syndrome of hashimoto thyroiditis are five related autoantibodies, namely, DR1, KDM1A, PCGF2, GPCPD1 and IGBP1. The serum-related autoantibody markers can reflect the syndrome characteristics of hashimoto thyroiditis, so that the traditional Chinese medicine differentiation is accurate, and the syndrome essence and molecular connotation of hashimoto thyroiditis can be disclosed.

Description

technical field [0001] The invention relates to the technical fields of immunology technology and serum antibody omics, and more specifically, the invention relates to a biomarker for detecting characteristic autoantibodies of different syndromes of Hashimoto's thyroiditis and its application. Background technique [0002] Hashimoto's thyroiditis (HT), also known as Hashimoto's disease (HD) or chronic lymphocytic thyroiditis (CLT), is the most common organ-specific autoimmune disease The disease, clinically manifested as diffuse enlargement of the thyroid gland and elevated levels of serum thyroid autoantibodies, is the most common cause of hypothyroidism, and it is reported that about 20%-30% of patients will develop hypothyroidism. At present, the incidence of HT is increasing rapidly year by year, and it has become a common disease that seriously threatens people's health, work and life. According to literature reports, the global annual incidence of HT is about 0.3-1.5 ...

Claims

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Application Information

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IPC IPC(8): G01N33/68A61K39/395A61P43/00
CPCG01N33/6854G01N33/6893A61K39/395A61P43/00G01N2800/046G01N2800/60Y02A50/30
Inventor 司富春宋雪杰司高司季青
Owner HENAN UNIV OF CHINESE MEDICINE
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