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Application of fructus polygoni orientalis extract in preparation of drugs for preventing or treating non-alcoholic fatty liver diseases

A water saffron, non-alcoholic technology, used in the water saffron extract in the pharmaceutical field

Pending Publication Date: 2021-11-23
XUZHOU NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is no report that the extract of safflower seed can be used alone as an active ingredient for the treatment of non-alcoholic fatty liver disease

Method used

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  • Application of fructus polygoni orientalis extract in preparation of drugs for preventing or treating non-alcoholic fatty liver diseases
  • Application of fructus polygoni orientalis extract in preparation of drugs for preventing or treating non-alcoholic fatty liver diseases
  • Application of fructus polygoni orientalis extract in preparation of drugs for preventing or treating non-alcoholic fatty liver diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 water safflower extract

[0030] 1. Preparation of Saffron Seed Extract

[0031] The dried medicinal material of Shuihonghuazi (purchased from Beijing Tongrentang, place of origin Bozhou, Anhui) was pulverized into a powder, 50g of the powder was weighed and soaked in 80% ethanol (100mL) for 2h, ultrasonically extracted at room temperature for 30min, and then the filtrate was collected by filtration. The filter residue was ultrasonically extracted again at room temperature for 30 min, and the filtrate was collected by filtration. The filtrates were combined and concentrated under reduced pressure to an oil.

[0032] 2. Liquid Chromatography-Mass Spectrometry Analysis of Safflower Extract

[0033]The obtained oil was dissolved in 80% methanol (5 mL), then centrifuged at 15,000×g for 15 min, the supernatant was taken, filtered with a 0.22 μm nylon membrane filter, and the sample solution was analyzed by liquid chromatography-mass spectrom...

Embodiment 2

[0037] Example 2 The improvement of NAFLD by the extract of Fructus Saffron

[0038] 1. Materials and Reagents

[0039] Sodium oleate, sodium palmitate, oil red O, bovine serum albumin (BSA) and brominated thiazolium tetrazolium (MTT) were purchased from Sigma Aldrich, human liver cell line LO 2 Cells were purchased from ATCC, 1640 medium for cell culture, penicillin-streptomycin double antibody solution and fetal bovine serum (FBS) were purchased from GIBCO Invitrogen, triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) detection kits were purchased from Nanjing Jiancheng Institute of Bioengineering, active oxygen fluorescent probe CM-H2DCFA was purchased from Molecular Probes, inflammatory factor TNF-α, IL-1β and IL-6 gene primers were purchased from From BGI.

[0040] 2. Experimental method

[0041] LO 2 Cells were cultured in 1640 medium (containing 10% FBS, 100 U / mL penicillin and 100 μg / mL streptomycin), plac...

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Abstract

The invention provides an application of a fructus polygoni orientalis extract as a unique active ingredient in preparation of drugs for preventing or treating non-alcoholic fatty liver diseases. In the invention, the fructus polygoni orientalis extract mainly contains flavone and polyphenolic acid compounds, and can effectively relieve lipid accumulation in free fatty acid induced human hepatocytes, reduce triglyceride and cholesterol levels, and reduce oxidative stress and inflammatory factor levels, which indicates that the fructus polygoni orientalis extract can have a remarkable treatment effect on the non-alcoholic fatty liver disease, and can be used for preparing medicines for preventing or treating non-alcoholic fatty liver diseases.

Description

technical field [0001] The present invention relates to a new application of the extract of Polygoni Orientalis Fructus, in particular to a new application of the extract of Polygoni Orientalis Fructus in the field of pharmacy. Use in drugs for chronic fatty liver disease. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is a metabolic stress-induced liver injury closely related to obesity, and its disease spectrum includes progression from non-alcoholic hepatic steatosis to non-alcoholic steatohepatitis ( non-alcoholic steatohepatitis, NASH), liver fibrosis and cirrhosis, eventually leading to hepatocellular carcinoma. With the increase of obesity, insulin resistance and metabolic syndrome, NAFLD has become the most common liver disease in developed countries, affecting about one-third of the global population, and is one of the huge and growing disease burdens facing the world. In my country, NAFLD has become the number one chronic liver disease an...

Claims

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Application Information

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IPC IPC(8): A61K36/704A61P1/16A61P3/06A61K131/00
Inventor 栗政陈凯李进冬周磊陈艳花慧莲李建华瞿建江
Owner XUZHOU NORMAL UNIVERSITY
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