Novel sweet-waxy corn and utilization method thereof
The technology of sweet and waxy corn and waxy corn is applied in the field of new sweet and waxy corn and its utilization, which can solve the problems of less seed content, low germination rate, weak seedling vigor, etc. Seedlings grow robustly
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Embodiment 1
[0041] Embodiment 1, sweet and waxy type fresh corn and hybrid breeding
[0042] 1. Artificial mutagenesis of sweet and waxy fresh corn
[0043] Utilize the existing artificial mutagenesis technology (see Zhou Hongsheng, Maize Seed Encyclopedia, China Agricultural Press, 2000, 217-219. The probability of recessive mutation is about 0.1%), mutagenesis with ethyl methanesulfonate (EMS) The pollen of waxy corn Shen Nuo 3 (Liao Nong Shen Zheng Zi No. 490) was treated with the agent. The specific method is to mix EMS and pollen 10 times the volume of pollen for pollen mutagenesis treatment, shake it every 3 minutes, and after 45 minutes of treatment, apply it on the filaments that have been bagged in advance with a brush, pollinate 30 ears, and then Bagged and isolated to obtain the first generation (M1) seeds of mutagenesis.
[0044] The seeds of the first generation of mutagenesis (M1) harvested in the previous year were sown and selfed to obtain the ears of the second generati...
Embodiment 2
[0056] Embodiment 2, Sweet waxy plus waxy type fresh corn and hybrid breeding
[0057] 1. Artificial mutagenesis of sweet and waxy fresh corn
[0058] Utilizing the existing artificial mutagenesis technology (see Zhou Hongsheng, Compendium of Maize Seeds, China Agricultural Press, 2000, 217-219;), the waxy corn Jingke Nuo 2000 was treated with ethyl methanesulfonate (EMS) mutagen (National Examination Jade 2010014) pollen. The specific method is to mix EMS and pollen 10 times the volume of pollen for pollen mutagenesis treatment, shake it every 3 minutes, and after 45 minutes of treatment, apply it on the filaments that have been bagged in advance with a brush, pollinate 30 ears, and then Bagged and isolated to obtain the first generation (M1) seeds of mutagenesis.
[0059] 2. Phenotype Identification and Tasting Validation
[0060] The seeds (M1) harvested in the previous year were sown and selfed to obtain the ears of the 2nd generation of mutagenesis (M2). According to ...
Embodiment 3
[0071] Embodiment 3. Breeding of new varieties of sweet and waxy type fresh corn
[0072] 1. Breeding of sweet and waxy fresh corn inbred lines
[0073] Using the existing cross-breeding technology, the sweet-waxy type fresh corn inbred line SW60 was used as the non-recurrent parent, and Shennuo 350 (the male parent of Shennuo 7) was used as the recurrent parent for backcrossing. First, SW60 was used as the male parent to cross, and from the second generation, Shennuo 350 was used as the male parent for backcrossing. After 5 rounds of trans-breeding, sweet and waxy fresh corn inbred lines were bred. The grains of this series are sunken, fuller than super sweet corn, the endosperm is powdery, non-waxy, opaque, non-shrinking, and the epidermis is dull and wrinkled. After fresh food tasting verification, it has a sweet and waxy taste. Obtain sweet waxy type fresh corn inbred line SW350.
[0074] 2. Preparation of sweet and waxy fresh corn hybrids
[0075] Sweet and waxy inbr...
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