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Industrial seedling raising method for feed type tetraploidy black locust

A tetraploid black locust, industrialized technology, applied in the field of agricultural biology, to achieve the effect of reducing pollution rate, less pests and diseases, and simple operation steps

Active Publication Date: 2018-08-28
SHANDONG FOREST SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be seen from the above that in the process of shoot culture and adventitious root induction, there are differences in the results of different laboratories in the selection and sterilization of explants, culture conditions and hormone ratios, and these studies are in a certain degree. Great achievements have been made in this field, which is limited to laboratory research, and it is far from enough for the industrialized seedling raising system to adopt tissue culture as a new feed-type tetraploid Robinia pseudoacacia species

Method used

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  • Industrial seedling raising method for feed type tetraploidy black locust
  • Industrial seedling raising method for feed type tetraploidy black locust
  • Industrial seedling raising method for feed type tetraploidy black locust

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Embodiment 1

[0057] One, the acquisition of tetraploid Robinia pseudoacacia aseptic test-tube plantlets, concrete steps are as follows:

[0058] 1. Selection and treatment of explants:

[0059] In April and May, when the tetraploid Robinia pseudoacacia has just grown 30-40cm new shoots, select a good individual plant that grows robustly and has typical traits, and cuts 20-30cm shoots from the top bud downward with fruit branch scissors, and removes excess stems and leaves. The cut branches are wrapped in wet newspaper, covered with plastic film, put into a foam box containing ice packs for refrigerated storage, and brought back to the tissue culture room for disinfection and sterilization as soon as possible.

[0060] The explants brought back are taken out, and the dust on the surface of the branches is gently rinsed with a soft brush dipped in a detergent solution. When scrubbing, the action should be light to avoid damaging the bud points. After washing, rinse with running water for 0...

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Abstract

The invention discloses an industrial seedling raising method for feed type tetraploidy black locust. The industrial seedling raising method comprises acquisition of sterile test tube seedlings, multiplying culture of the test tube seedlings, and rooting and acclimatization and transplanting technology of the test tube seedlings. The invention provides an initial medium, a tube seedling multiplying medium and a rooting medium, thus the monthly multiplying coefficient of the test tube seedlings can reach 10-12, the number of cluster bud seedlings on a callus is 10-15 and even up to 22, and therooting rate reaches 95% and above; during the acclimatization and transplanting, through the river sand and the mixed seedling raising matrix in an acclimatization nutrition pot, the acclimatizationsurvival rate goes reaches 95% and above, and the transplanting survival rate reaches 100%. Therefore, the method disclosed by the invention solves the problems of generally low multiplying efficiencyand low survival rate of the feed type tetraploidy black locust; since the technology is easy to operate, the method can realize rapid multiplication of the tetraploidy black locust to achieve an aimof fast popularization.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for industrialized seedling cultivation of feed-type tetraploid Robinia pseudoacacia. Background technique [0002] Feed-type tetraploid black locust (Tetraploidy Black Locust) is an excellent clone of Robinia pseudoacacia introduced from South Korea to my country by Beijing Forestry University in 1997. . Its leaves are wide and thick, with high protein content, tender and juicy, and good palatability. It is an excellent feed tree species. Relying on the national "863" project undertaken by the Shandong Academy of Forestry Sciences and the Shandong Provincial Agricultural Variety Industrialization Development Project, the forage-type tetraploid Robinia pseudoacacia was used as a material. After 15 years of systematic breeding, the tissue culture seedlings were screened for salt tolerance, Planted in different areas of coastal saline-alkali land and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/10A01G24/23A01G24/28
CPCA01H4/001A01H4/005A01H4/008
Inventor 李双云夏阳付茵茵庞彩红刘盛芳臧真荣亓玉昆
Owner SHANDONG FOREST SCI RES INST
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