Formula, preparation method and use method of biological preservative for chilled beef
A biological preservative and beef technology, applied in the direction of chemical preservation of meat/fish, etc., can solve the problems of spoilage, short shelf life, protein oxidation, etc., and achieve the effect of preventing juice loss, extending shelf life, and preventing bacterial invasion
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[0029] The preparation method of the modified sodium alginate antibacterial liquid is as follows: dissolve 0.4 to 1.6 parts of polyaldehyde sodium alginate in 20 parts of pure water according to parts by mass, adjust the pH to 6.0 to 6.5 with gluconic acid solution, and then add 0.02 ~0.1 part of lysozyme, 0.04~0.2 part of kojic acid, stirred and reacted at 32~40°C for 0.5~2h, then added 0.01~0.05 part of Artemisia annua extract, stirred evenly, and cooled to 0~4°C to obtain the described Modified sodium alginate antibacterial liquid.
[0030] The preparation method of the Artemisia annua extract is as follows: after washing and grinding the Artemisia annua leaves, add them into an ethanol solution with a volume concentration of 70-80% according to a solid-to-liquid ratio of 1g:10mL, reflux in a water bath at 95°C for 1-2 hours, After filtration, the filtrate was concentrated under reduced pressure and dried under vacuum to obtain the Artemisia annua extract.
[0031] The pre...
Embodiment 1~5
[0033] Embodiments 1-5 study the effect of lysozyme dosage on the quality of chilled fresh beef
Embodiment 1
[0035] (1) Preparation of Artemisia annua extract: After washing and grinding the Artemisia annua leaves, add them to an ethanol solution with a volume concentration of 80% according to a solid-to-liquid ratio of 1g:10mL, reflux in a water bath at 95°C for 2h, filter, and reduce the filtrate to concentrated under pressure and dried in vacuum to obtain the Artemisia annua extract;
[0036] (2) Preparation of modified sodium alginate antibacterial liquid: Dissolve 1.2 parts of polyaldehyde sodium alginate in 20 parts of pure water in parts by mass, adjust the pH to 6.2 with gluconic acid solution, then add 0.02 parts of lysozyme, 0.20 parts of Kojic acid, stirred and reacted at 40°C for 1 hour, then added 0.02 parts of Artemisia annua extract, stirred evenly, and cooled to 4°C to obtain the modified sodium alginate antibacterial liquid;
[0037] (3) Preparation of biological preservatives: add 0.01 part of nisin, 0.01 part of ε-polylysine, 0.008 part of tea polyphenol, and 0.01 ...
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