Viscosity reducer for oil exploitation and preparation method thereof
A technology for viscosity reducer and petroleum, applied in the field of viscosity reducer for petroleum development and its preparation, can solve the problems of high viscosity and difficult to degrade heavy oil, few studies on hydrocarbon-solving bacteria, difficult to control the proportion, etc., and achieves good emulsion stability. , the effect of promoting the application value and improving the efficiency
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[0034] A preparation method of a viscosity reducer for petroleum exploitation, comprising the steps of:
[0035] (1) Preparation of microbial inoculum:
[0036] A. Take the microbial strains and inoculate them in the LB medium, carry out the activation treatment, carry out the sonic treatment of 30~50kHz at the same time of the activation treatment, after the completion, the microbial bacterial liquid is ready for use, and the components and the corresponding weight percentages in the microbial strains are: 10-12% of Bacillus licheniformis, 10-12% of Bacillus subtilis, 6-10% of Rhodococcus, and the balance is XJBM Pseudomonas aeruginosa;
[0037] B. Subculture the activated microbial bacterium liquid in operation A;
[0038] C. Expand the culture of the microbial liquid obtained after subculture in operation B, and then perform centrifugation-vortex-centrifugation on the obtained bacterial liquid alternately, centrifuge at 4000-5000rpm for 10-16min, and vortex at 2000-3000rpm...
Embodiment 1
[0049] A preparation method of a viscosity reducer for petroleum exploitation, comprising the steps of:
[0050] (1) Preparation of microbial inoculum:
[0051]A. Get microbial strains and inoculate them in LB culture medium, carry out activation treatment, carry out 30kHz acoustic wave treatment at the same time of activation treatment, after finishing, obtain microbial bacterial liquid standby, each composition and corresponding weight percentage in the microbial strains are: licheniformis spores Bacillus 10%, Bacillus subtilis 10%, Rhodococcus 6%, the rest is XJBM
[0052] Pseudomonas aeruginosa;
[0053] B. Subculture the activated microbial bacterium liquid in operation A;
[0054] C. Expand the culture of the microbial bacterial liquid obtained after subculture in operation B, and then perform centrifugation-vortex-centrifugation alternately on the obtained bacterial liquid, centrifuge at 4000rpm for 10min, vortex at 2000rpm for 3min, centrifuge at 8000rpm for 6min, an...
Embodiment 2
[0064] A preparation method of a viscosity reducer for petroleum exploitation, comprising the steps of:
[0065] (1) Preparation of microbial inoculum:
[0066] A. Take the microbial strains and inoculate them in the LB medium, carry out the activation treatment, carry out the sonic treatment of 30~50kHz at the same time of the activation treatment, after the completion, the microbial bacterial liquid is ready for use, and the components and the corresponding weight percentages in the microbial strains are: Bacillus licheniformis 11%, Bacillus subtilis 11%, Rhodococcus 8%, the rest is XJBM
[0067] Pseudomonas aeruginosa;
[0068] B. Subculture the activated microbial bacterium liquid in operation A;
[0069] C. Expand the culture of the microbial liquid obtained after subculture in operation B, and then perform centrifugation-vortex-centrifugation alternately on the obtained bacterial liquid, centrifuge at 4500rpm for 13min, vortex at 2500rpm for 4min, centrifuge at 8500rpm...
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