SSR (Simple Sequence Repeat) molecular marker of new fruit mulberry variety 'Yueshen 201', core primer group of SSR molecular marker, kit and application of SSR molecular marker, or core primer group or kit

Abstract

The invention discloses an SSR (Simple Sequence Repeat) molecular marker of a new fruit mulberry variety 'Yueshen 201', a core primer group of the SSR molecular marker, a kit and an application of the SSR molecular marker, or the core primer group or the kit. A fluorescent SSR technology is utilized, 300 pairs of SSR primers developed through mulberry genome sequence design are screened, it is confirmed that core primers marked with M20310 and M252 can amplify two specific bands for the new fruit mulberry variety 'Yueshen 201' respectively, the new fruit mulberry variety 'Yueshen 201' can be identified from fruit mulberry strains respectively, and the new fruit mulberry variety 'Yueshen 201'can be identified from the fruit mulberry strains respectively. The molecular marker can be used for rapid identification and detection of the new fruit mulberry variety 'Yueshen 201'. According to the invention, the M20310 marker and/or the M252 marker of the 'Yueshen 201' are/is directly detected to identify the 'Yueshen 201', the uncertainty of external morphological characteristics on identification of the 'Yueshen 201' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry variety 'Yueshen 201', and the molecular marker, the core primer group and the kit are beneficial to popularization, utilization and protection of the varieties.

Description

technical field [0001] The invention relates to the technical field of identification of variety resources and germplasm innovation, in particular to the SSR molecular marker of a new fruit mulberry variety 'Yuechen 201' and its core primer set, kit and application. Background technique [0002] Fruit mulberry is the purpose of mulberry fruit production, and it is collectively referred to as mulberry trees for fruit or leaf use. It is one of the important types of rich mulberry germplasm resources in my country. As a new type of fruit tree, fruit mulberry is loved by people because of its unique taste, rich nutrition and high active substances. The planting area and area are constantly expanding, and it has shown a good development prospect. In addition to fresh food, mulberries are processed and developed into mulberry juice, mulberry wine, mulberry jam, mulberry red pigment, mulberry vinegar and other products. The mulberry industry has begun to take shape and has shown a ...

AI Technical Summary

Problems solved by technology

[0005] The traditional identification of new mulberry varieties is mainly based on phenotypic traits, which is greatly affected by the environment, poor stability, and long testing cycle, which seriously affects the effectiveness and authority of new variety identification

Images