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Development of polypeptide antibiotic antibacterial artificial dressing

A polypeptide antibiotic and artificial technology, applied in bandages, medical science, etc., can solve problems such as antibiotic resistance, difficulty in inhibiting bacterial infection, and failure of antibacterial artificial dressings

Inactive Publication Date: 2021-11-12
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The lack of antibacterial properties of chitosan-based artificial dressings makes it difficult to achieve the purpose of inhibiting bacterial infection
Antibacterial artificial dressings added with antibiotics can inhibit infection to a certain extent, but bacteria are prone to develop resistance to antibiotics, making antibacterial artificial dressings ineffective; heavy metals such as silver have excellent antibacterial properties and low drug resistance, but they have significant Biological toxicity, unable to meet clinical needs

Method used

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  • Development of polypeptide antibiotic antibacterial artificial dressing
  • Development of polypeptide antibiotic antibacterial artificial dressing
  • Development of polypeptide antibiotic antibacterial artificial dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Preparation of chitosan matrix: Add 5g of chitosan into 80mL of 2% acetic acid solution, use a stirrer to swell evenly at a speed of 500r / min, after completely dissolving, test the pH value, use 1mol / L NaOH adjusts the pH of the solution to 4.5-5, records the amount of NaOH added to the solution, and stores it in the dark. Record it as solution A.

[0019] 2. Preparation of antimicrobial peptide solution: Dissolve 5 mg of antimicrobial peptide in 18 mL of aqueous solution and configure it into a 5 mg / 18 mL aqueous solution. In subsequent orthogonal experiments, 1 mL of 1% peptide is added accordingly. Labeled as Solution B.

[0020] 3. Vitamin C solution preparation: Boil deionized water to remove air bubbles, cover with plastic wrap, and use to prepare the solution after cooling. Take 1g of vitamin C, add boiled and cooled deionized water, and prepare a 0.1g / mL vitamin C solution. Record it as solution C.

[0021] 4. Preparation of mixed solution: Dissolve 0.3g ...

Embodiment 2

[0024] 1. Preparation of chitosan matrix: Add 5g of chitosan into 80mL of 2% acetic acid solution, use a stirrer to swell evenly at a speed of 500r / min, after completely dissolving, test the pH value, use 1mol / L NaOH adjusts the pH of the solution to 4.5-5, records the amount of NaOH added to the solution, and stores it in the dark. Record it as solution A.

[0025] 2. Preparation of antimicrobial peptide solution: Dissolve 5 mg of antimicrobial peptide in 18 mL of aqueous solution and configure it into a 5 mg / 18 mL aqueous solution. In subsequent orthogonal experiments, 1 mL of 1% peptide is added accordingly. Labeled as Solution B.

[0026] 3. Vitamin C solution preparation: Boil deionized water to remove air bubbles, cover with plastic wrap, and use to prepare the solution after cooling. Take 1g of vitamin C, add boiled and cooled deionized water, and prepare a 0.1g / mL vitamin C solution. Record it as solution C.

[0027] 4. Preparation of mixed solution: Dissolve 0.3g ...

Embodiment 3

[0030] 1. Preparation of chitosan matrix: Add 5g of chitosan into 80mL of 2% acetic acid solution, use a stirrer to swell evenly at a speed of 500r / min, after completely dissolving, test the pH value, use 1mol / L NaOH adjusts the pH of the solution to 4.5-5, records the amount of NaOH added to the solution, and stores it in the dark. Record it as solution A.

[0031] 2. Preparation of antimicrobial peptide solution: Dissolve 5 mg of antimicrobial peptide in 18 mL of aqueous solution and configure it into a 5 mg / 18 mL aqueous solution. In subsequent orthogonal experiments, 1 mL of 1% peptide is added accordingly. Labeled as Solution B.

[0032] 3. Vitamin C solution preparation: Boil deionized water to remove air bubbles, cover with plastic wrap, and use to prepare the solution after cooling. Take 1g of vitamin C, add boiled and cooled deionized water, and prepare a 0.1g / mL vitamin C solution. Record it as solution C.

[0033] 4. Preparation of mixed solution: Dissolve 0.3g ...

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Abstract

The invention relates to development of a polypeptide antibiotic antibacterial artificial dressing. The dressing is composed of antibacterial peptide, a mixed solution and a substrate, wherein the substrate is composed ofthe following components in percentage by weight: 3-6% of chitosan, 0.3-0.9% of collagen, 0.1-0.3% of hyaluronic acid, and 3-6% of glycerin; the substrate is prepared by the following steps: adding 3-6% of chitosan into 80-100mL of 2% acetic acid solution, performing uniform swelling by using a stirrer at the rotating speed of 500 r / min, testing the pH value, and adjusting the pH value of the solution to 4.5-5 by using 1 mol / L of NaOH; and dissolving 0.3-0.9% of collagen peptide into the mixed solution at room temperature, adding 0.1-0.3% of hyaluronic acid into the mixed solution, and performing uniform swelling at the rotating speed of 500 r / min, wherein the mixed solution is prepared from 3-6% of glycerin, 1-3% of a polypeptide solution, 1% of a vitamin C solution and 11-13% of deionized water. The finally prepared novel polypeptide antibiotic-based antibacterial artificial dressing is light yellow non-transparent gel. The gel has good smearing performance, and can be directly applied to wounds to achieve antibacterial and healing-promoting effects.

Description

technical field [0001] The invention relates to a preparation method of a gel dressing, in particular to a preparation method of a polypeptide antibiotic antibacterial dressing. Background technique [0002] The skin is a natural barrier between the human body and the external environment, which can protect the internal tissues and organs of the human body from various physical, chemical and biological invasions. In general, skin has a strong regenerative capacity and can heal itself when damaged. However, due to skin wounds in wars, burns, and large-scale skin damage in daily life, the dermis of the skin is damaged and the healing speed is slow, so artificial dressings are needed for temporary wound protection during treatment. [0003] Chitosan is a natural polymer material with good biocompatibility. In addition, as an artificial dressing, chitosan is also soft, comfortable, and has a good fit with the wound; and the chitosan-based artificial dressing is breathable, wat...

Claims

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Application Information

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IPC IPC(8): A61L26/00
CPCA61L26/0066A61L26/008A61L26/0023A61L2300/406A61L2300/252C08L5/08
Inventor 胡云睿肖秋群周曼珍张信果张巧玲王琳
Owner SOUTHERN MEDICAL UNIVERSITY
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