Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora of piglets

A technology of traditional Chinese medicine oral liquid and intestinal flora, which is applied in the direction of antibacterial drugs, medical formulas, drug combinations, etc., can solve problems affecting the survival rate of piglets, endogenous infection of animals, and destruction of micro-ecological balance, so as to improve piglets Effects of intestinal flora, treatment of piglet diarrhea, and improvement of growth performance

Active Publication Date: 2021-11-09
KUNMING UNIV OF SCI & TECH
View PDF12 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term and large-scale use of antibiotics has destroyed the micro-ecological balance of the body, leading to the emergence of drug-resistant bacteria and bacterial mutations
Seriously affect the survival rate of piglets, and easily cause animal endogenous infection or secondary infection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora of piglets
  • Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora of piglets
  • Fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora of piglets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: The preparation of this treatment of piglet diarrhea and improvement of its intestinal flora fermented traditional Chinese medicine oral liquid

[0020] (1) Resuscitate the strains preserved in a volume ratio of 50% glycerol:bacteria solution=7:3 in a water bath at 37°C for 1 min, and culture the revived Lactobacillus salivarius, Lactococcus lactis, and Lactobacillus plantarum in the MRS with an inoculation loop Streak on the base, streak the Bacillus licheniformis species on the LB medium with an inoculation loop, and then place it in a 37°C constant temperature incubator for 12 hours; pick a single colony and inoculate it into a sterile liquid medium, and Cultured on a shaker to OD 600 The value is 0.6~0.8, subculture the bacterial solution with 1% inoculum, and then inoculate the subcultured bacterial solution into the required amount of sterile liquid medium for cultivation at 37°C to obtain an effective viable count of 10 8 ~10 9 CFU / mL Lactobacillus s...

Embodiment 2

[0024] Example 2: The preparation of this treatment of piglet diarrhea and improvement of its intestinal flora fermented traditional Chinese medicine oral liquid

[0025](1) Resuscitate the strains preserved in a volume ratio of 50% glycerol:bacteria solution=7:3 in a 37°C water bath for 1 min, and use an inoculation loop to inoculate the strains of Lactobacillus salivarius, Lactococcus lactis, and Lactobacillus plantarum Streak on the MRS medium, use an inoculation loop to streak the Bacillus licheniformis species on the LB medium, and then place it in a constant temperature incubator at 37 ° C for 12 hours; pick a single colony and shake it in a sterile liquid medium bed culture to OD 600 value of 0.6~0.8, subculture the bacterial solution with 1% inoculum, and then inoculate the subcultured bacterial solution into a sterile liquid medium for cultivation at 37°C to obtain an effective viable count of 10 8 ~10 9 CFU / mL Lactobacillus salivarius liquid, effective number of vi...

Embodiment 3

[0029] Example 3: Tolerance of 4 strains in the compound probiotic bacterial liquid to simulated gastrointestinal fluid

[0030] Preparation of artificial gastric juice: Take 1.64mL of 1mol / L dilute hydrochloric acid in a container, add 80mL of water, add 1g of pepsin, adjust the pH to 3.0, mix well and set the volume to 100mL, sterilize with a 0.22μm filter membrane; prepare pH4.0 in the same way Artificial gastric juice, placed in a 4°C refrigerator for later use;

[0031] Preparation of artificial intestinal juice: Take 0.68g of potassium dihydrogen phosphate, add 80mL of water to dissolve, add 1g of trypsin, adjust the pH to 6.8 with 0.1mol / L sodium hydroxide solution; Make simulated intestinal juice and store it in a 4°C refrigerator for later use;

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving intestinal flora of piglets. The fermented traditional Chinese medicine oral liquid is prepared by the following steps: uniformly mixing 1-1.5 kg of a traditional Chinese medicine composition, 0.8-1.2 L of a compound probiotic liquid, 0.5-1 kg of brown sugar and 0.3-0.6 kg of poplar flowers, regulating the water content to 55-65%, and carrying out sealing and fermenting at 37 DEG C for 6-8 days to complete traditional Chinese medicine fermentation; when a traditional Chinese medicine extracting solution is prepared, continuously adding 0.8-1.2 L of the compound probiotic liquid and 0.5-0.7 L of deionized water into the fermented traditional Chinese medicine, carrying out uniform mixing, standing and soaking at 23-26 DEG C and carrying out extracting for 24-30 hours, carrying out stirring irregularly during standing and soaking, and carrying out filtering by using a filter screen to remove filter residues so as to obtain the fermented traditional Chinese medicine oral liquid. The fermented traditional Chinese medicine oral liquid disclosed by the invention fully achieves the effects of probiotics, traditional Chinese medicines and fermentation products thereof, can achieve the effects of obviously treating piglet diarrhea, improving intestinal flora of piglets, reducing bacterial drug resistance and antibiotic drug residues caused by abuse of feed antibiotics and ensuring the food production safety. The fermented traditional Chinese medicine oral liquid disclosed by the invention is simple in preparation method and suitable for industrial production and market popularization and application.

Description

technical field [0001] The invention belongs to the technical field of compound probiotic preparations, and in particular relates to a fermented traditional Chinese medicine oral liquid for treating piglet diarrhea and improving its intestinal flora. Background technique [0002] Piglets are the core of modern pig production, and their health status has an extremely important impact on later growth and development. Since the piglet's own immune system is not fully developed, the invasion of harmful microorganisms can easily cause diarrhea in piglets. Piglets within one month of birth, due to incomplete development of digestive glands and thermoregulatory centers, such as temperature changes and excessive milk fat, can easily cause indigestion and diarrhea. Diarrhea is a common disease in pig farms, which can lead to a decrease in the survival rate of piglets and affect the composition of intestinal flora. Abnormal intestinal flora can also easily cause diarrhea and bring hu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/884A61K9/08A61P1/12A61P1/00A61P31/04A61K35/747A61K35/744A61K35/742
CPCA61K36/484A61K36/718A61K36/282A61K36/752A61K36/734A61K36/346A61K36/355A61K36/233A61K36/288A61K36/855A61K36/704A61K36/315A61K36/195A61K36/78A61K36/539A61K36/744A61K36/536A61K36/284A61K36/756A61K36/884A61K36/076A61K36/481A61K35/747A61K35/744A61K35/742A61K9/0095A61P1/12A61P1/00A61P31/04A61K2236/19A61K2300/00
Inventor 林连兵徐美余朱付千何秀邓先余张棋麟王峰郭军
Owner KUNMING UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com