Method for simultaneously determining various phenolic acids in honeysuckle

Abstract

The invention belongs to the technical field of analysis and detection, and particularly relates to a method for simultaneously determining various phenolic acids in honeysuckle, which adopts ultra-high performance liquid chromatography tandem mass spectrometry for determination, adopts a Hola C18 chromatographic column as a chromatographic column, adopts 0.08% formic acid aqueous solution-acetonitrile as a mobile phase, and adopts gradient elution; the detection wavelengths are 254 nm and 327 nm, the flow velocity is 0.3 ml/min, and the sample size is 2 mul; the electrospray ionization ion source scans in a positive and negative ion mode, the auxiliary gas temperature is 350 DEG C, the flow velocity is 8 L/min, the pressure is 35 psig, the spray voltage is 3000 V, the splitting voltage is 175 V, and the scanning range is m/z 50-800. According to the method, the phenolic acid components in the honeysuckle are accurately identified, the corresponding retention time of each component is obtained, and subsequent researchers can qualitatively research the phenolic acid components of the honeysuckle according to the method and the result provided by the invention without using expensive standard substances.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a method for simultaneously measuring multiple phenolic acids in honeysuckle. Background technique [0002] Honeysuckle (Lonicera japonica Flos) is a traditional Chinese medicine with high value and wide application. It is often used to evacuate wind-heat and clear away heat and detoxify. The effective active ingredients of honeysuckle mainly include phenolic acids, flavonoids and volatile oils, etc. Among them, phenolic acids have attracted extensive attention in recent years because of their good antioxidant activity, antiviral, antibacterial, anti-inflammatory, antioxidative and antitumor pharmacological activities. . In addition, the 2020 edition of "Chinese Pharmacopoeia" has also increased the content requirements for phenolic acids in honeysuckle, so phenolic acids as the main components of honeysuckle need to be further studied. [0003] With ...

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Problems solved by technology

In addition, the 2020 edition of "Chinese Pharmacopoeia" has also increased the content requirements for phenolic acids in honeysuckle. Therefore, as the main components of honeysuckle, phenolic acids need to be further studied

[0003] With the rapid improvement of natural compound separation and structure identification technology in recent years, more and more phenolic acids in honeysuckle have been discovered one after another. Qualitative and quantitative studies have been carried out, but there is little research on the identification of trace phenolic acids in honeysuckle, and there are many isomers in honeysuckle phenolic acids, so even if combined with mass spectrometry, it is difficult to pass the molecular weight. Determine the names of its components, which is not conducive to the development of honeysuckle resources and further research on its active components

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