Method for extracting squalene from schizochytrium limacinum and application of squalene in moisturizing and anti-aging cosmetics

A technology of Schizochytrium and squalene, which is applied in the direction of cosmetics, cosmetic preparations, and microbial-based methods, can solve the problems of high cost and low extraction yield of squalene, and reduce recycling costs and potential safety hazards , the effect of increasing production

Pending Publication Date: 2021-11-05
SHANTOU QIWEI IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to provide a method for extracting squalene from Schizochytrium and the application of squalene in moisturizing and anti-aging cosmetics, aiming to solve the problems of low yield and high cost of squalene extraction at present

Method used

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  • Method for extracting squalene from schizochytrium limacinum and application of squalene in moisturizing and anti-aging cosmetics
  • Method for extracting squalene from schizochytrium limacinum and application of squalene in moisturizing and anti-aging cosmetics
  • Method for extracting squalene from schizochytrium limacinum and application of squalene in moisturizing and anti-aging cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0040] A method for extracting squalene from Schizochytrium, comprising the following steps:

[0041] S1, cultivation of Schizochytrium:

[0042] S11. Transfer the Schizochytrium to the inclined surface, culture it at 25°C for 3-4 days, activate the Schizochytrium, then pick the activated Schizochytrium with an inoculation loop, insert it into the primary seed medium for Cultivate at 25°C and 200r / min for 2 days to obtain first-class seeds, and finally take the first-class seeds and insert them into the medium of second-class seeds, and cultivate at 25°C and 200r / min for 2 days to obtain second-class seeds; among them, the first-class seeds are cultivated Both base and secondary seed medium consisted of 30g / L glucose, 10g / L yeast extract and 15g / L minerals; g / LK2SO4, 0.75g / LKH2PO4, 0.7g / L(NH4)2SO4 and 0.3g / LFeSO4·7H2O;

[0043] S12. Transfer the secondary seeds to the fermentation medium in the fermentation mechanism according to the inoculum amount of 6% for fermentation an...

experiment example 1

[0076] The content of squalene extracted in the above examples and comparative examples was measured, and the measurement results are shown in Table 1 below.

[0077] Table 1 Determination results of squalene content

[0078]

[0079]

[0080] It can be seen from Table 1 that the content of biomass and extracted squalene in the fermentation broth of the embodiment is relatively high. Wherein, in the fermentation medium of comparative example 1, no multivitamin was added, and its squalene content was significantly lower; while in comparative examples 2 to 5, when a variety of different vitamins were used, the squalene content increased to some extent, but still slightly lower than the examples. In addition, it can be seen from Example and Comparative Example 6 that when the method of adding glucose in batches is adopted, the content of squalene also increases to a certain extent, because adding glucose in batches can avoid the inhibition of bacterial growth caused by the...

experiment example 2

[0082] The squalene extracted above is used in moisturizing anti-aging cosmetics, wherein, the moisturizing anti-aging cosmetics of the embodiment is made of the following raw materials in mass percentage: cosmetic active substance 0.8%, emulsifier 5%, oil 18%, moisturizing The squalene 0.4% that 6%, the embodiment extracts, the balance is deionized water. The blank control group is different from the examples in that it does not add squalene. The moisturizing and anti-aging effects of the cosmetics were tested, and the specific test results are shown in Table 2 below.

[0083] Table 2 Moisturizing and anti-aging test results

[0084]

[0085] It can be seen from Table 2 that the squalene extracted by the present invention has good moisturizing effect and anti-aging effect when used in cosmetics. Concrete, added 0.4% squalene in the embodiment, its skin moisture content value is higher, and the change value declines more slowly, it can be seen that its moisturizing effect...

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Abstract

The invention relates to a method for extracting squalene from schizochytrium limacinum and application of squalene in moisturizing and anti-aging cosmetics. The method comprises the following steps: activating the schizochytrium limacinum, inoculating the activated schizochytrium limacinum into a first-stage seed culture medium for culturing to obtain first-stage seeds, and inoculating the first-stage seeds into a second-stage seed culture medium for culturing to obtain second-stage seeds; inoculating the secondary seeds into a fermentation culture medium for fermentation culture to obtain fermentation liquor; adding alkaline protease into the fermentation liquor, and performing enzymolysis wall breaking on the schizochytrium limacinum; adding absolute ethyl alcohol into the wall-broken bacterial liquid, removing a supernatant water-ethyl alcohol phase through oscillation centrifugal separation, and collecting bottom bacterial sludge; adding normal hexane into the bottom bacterial sludge, oscillating and centrifuging to collect supernate, repeating for a plurality of times until the supernate is colorless and transparent, finally evaporating to remove the normal hexane, and collecting to obtain a squalene crude product; and degumming, deacidifying, bleaching and deodorizing the squalene crude product to obtain purified squalene. The method solves the problems of low squalene extraction yield and high cost at present.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a method for extracting squalene from Schizochytrium and the application of squalene in moisturizing and anti-aging cosmetics. Background technique [0002] Squalene is a highly unsaturated straight-chain triterpenoid compound formed by linking 6 isoprenes. It is also known as squalene, tricosahexaene, cod liver oil terpene or squalene. The system is named 2,6 ,10,15,19,23-Hexamethyl-2,6,10,14,18,22-tetracosahexaene, molecular formula C 30 h 50 , Light yellow or colorless oily liquid at room temperature. Squalene has biological activities such as improving hypoxia tolerance, inhibiting microbial growth, antibacterial and anti-inflammatory, and regulating cholesterol metabolism. Moreover, squalene is a non-toxic enhancer with anti-aging and anti-cancer effects. [0003] Squalene has a wide range of sources and exists in microorganisms, plant seeds, microalgae, shark liver an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C12N1/14C12M1/40C12M1/38C12M1/34C12M1/02C12M1/00C07C7/00C07C11/21A61K8/31A61Q19/00A61Q19/08C12R1/645
CPCC12P5/007C12N1/14C12M21/18C12M23/48C12M27/02C12M41/22C12M47/10C07C7/005A61K8/31A61Q19/00A61Q19/08A61K2800/85A61K2800/805C07C11/21Y02E50/30
Inventor 周坤江
Owner SHANTOU QIWEI IND CO LTD
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