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Fermentation improvement method of feed peony seed meal and fermentation product and application thereof

A technology of peony seed meal and fermentation products, which is applied in the field of fermentation improvement of peony seed meal for feeding, can solve the problems of vitamin absorption and poor digestion, interference with the operation of the digestive system, liver failure and other problems, and achieve the enhancement of intestinal flora The effects of diversity, promotion of stable development, and reduction of morbidity

Pending Publication Date: 2021-11-05
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, experiments by Zhang Yaxin and others proved that saponins have certain toxicity, and long-term consumption will damage the liver and lead to liver failure
Studies have also found that saponins produced by certain plants may interfere with the functioning of the digestive system, adversely affecting the absorption and digestion of fat, glucose, protein, cholesterol and vitamins

Method used

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  • Fermentation improvement method of feed peony seed meal and fermentation product and application thereof
  • Fermentation improvement method of feed peony seed meal and fermentation product and application thereof
  • Fermentation improvement method of feed peony seed meal and fermentation product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This embodiment provides a method for improving the fermentation of forage peony seed meal, which mainly includes the following steps:

[0043] (1) Pulverization treatment: put the peony seed dregs into a pulverizer for pulverization, pass through a 60-mesh sieve, and store for later use;

[0044] (2) Saponin extraction: first draw a standard curve of oleanolic acid, weigh 2 mg of oleanolic acid standard substance in a small beaker, add a small amount of absolute ethanol, shake well to dissolve, and then transfer to a 10ml volumetric flask. Dilute to volume with absolute ethanol to prepare a 0.2 mg / ml oleanolic acid reference solution. Prepare 3 groups of test tubes (6 in each group, numbered 1-1, 1-2, 1-3, 1-4, 1-5, 1-6, and the remaining two groups refer to the above method by analogy), add 0ml, 0.1ml, 0.2ml, 0.3ml, 0.4ml, 0.5ml oleanolic acid reference substance dissolved branch solution. After each test tube was evaporated in a water bath, 0.5 ml of 5% vanillin-gl...

Embodiment 2

[0050] The difference from Example 1 is that yeast liquid is selected as the strain required for microbial fermentation.

Embodiment 3

[0052] The difference from Example 1 is that the strains required for microbial fermentation are a mixed bacterial solution obtained by mixing activated Bacillus velesi and yeast in a ratio of 1:1.

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Abstract

The invention relates to a fermentation improvement method of feed peony seed meal and a fermentation product and application thereof. The fermentation improvement method mainly comprises the following steps: crushing treatment, strain activation and fermentation treatment, i.e., the process of degrading saponin by microbial fermentation of peony seed meal is completed. In addition, the invention further provides a preparation method of an enzyme capable of degrading the saponin in the peony seed meal. The method can rapidly extract the saponin, utilize microorganisms to degrade the saponin in the peony seed meal, find out the enzyme for degrading the saponin in the peony seed meal, fully process and utilize the peony seed meal and lay a foundation for increasing the proportion of the peony seed meal added into a laying hen feed in the future, is convenient for development and utilization of peony seed meal resources, reduces waste of the peony seed meal resources and environmental pollution, is beneficial to promotion of stable development of the oil peony industry, and is simple and low in cost.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for improving the fermentation of forage peony seed dregs, as well as its fermentation product and application. Background technique [0002] Peonies for oil are widely planted in my country, and peonies for oil are mainly distributed in Anhui Province, Shaanxi Province and Qinling Taibai Mountains in Gansu Province. It takes only 4 to 5 years for my country's oil peony to reach the peak production period, and it can maintain the peak production period for more than 30 years. The seed yield per mu is also very objective, which can reach more than 350-400 catties, and the oil yield is 80-100 catties. [0003] However, the development and research of peony seed meal in my country is not common at present, and most of them are discarded as residues after extracting peony seed oil, which makes resources seriously wasted and the ecological environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12P1/04C12N9/00A23K10/12C12R1/07C12R1/645
CPCC12P1/02C12P1/04C12N9/00A23K10/12
Inventor 王在贵肖芬芬聂铭王华斌陶伟聂琬晴聂蕾项子琳陈茜雅詹凯聂京荣
Owner ANHUI AGRICULTURAL UNIVERSITY
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