Microorganism separation and detection system and detection method

A detection system and microbial technology, applied in the field of microbial detection, can solve problems such as inability to achieve on-site detection, and achieve the effects of increasing the probability of collision and binding, fast response, and improving capture efficiency

Active Publication Date: 2021-11-02
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, none of these three methods can achieve on-site detection

Method used

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  • Microorganism separation and detection system and detection method
  • Microorganism separation and detection system and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] Embodiment 1 The construction of microorganism separation and detection system / platform

[0127] The microorganism separation and detection system provided in this embodiment, the system includes a circular microfluidic chip 5, a rotating (high gradient) magnetic field device 15, connecting elements, a stepping motor 16, a stepping motor driver 17, a fixing device 19, Embedded device (single chip microcomputer) 18 and smartphone image analysis device 20 .

[0128] The annular microfluidic chip is provided with six spindle-shaped liquid storage chambers 2 and an arc-shaped reaction chamber 3 connected to the outlet of each liquid storage chamber (the arc-shaped reaction chamber is arranged on the outer edge of the chip), and the storage chamber The liquid chamber is used to store the loaded liquid, and the arc-shaped reaction chamber is used for the separation and detection of target microorganisms;

[0129] The rotating magnetic field device and the annular microfluidi...

Embodiment 2

[0165] Example 2 Rapid separation and detection method of food-borne pathogenic bacteria based on nano-nickel wire mesh and HRP-nanoflower catalytic color development

[0166] In this embodiment, using the foodborne pathogenic bacteria detection platform constructed in Example 1, firstly, the modified immune nano-nickel wire solution, the sample solution to be tested containing the target microorganism, the HRP-nano flower solution, and the cleaning solution 1 ( PBST solution), cleaning solution 2 (PBST solution) and TMB chromogenic solution (commercialized TMB chromogenic solution) are preloaded in the spindle-shaped liquid storage cavity near the center of the circular microfluidic chip, and the solution is transferred by centrifugal force In the reaction chamber on the outer ring of the circular microfluidic chip, each reaction solution is separated by air and an independent liquid section is formed. Then, the nano-nickel wire is moved by the high-gradient magnetic field ro...

Embodiment 3

[0198] 1. For verification, use blue TMB OX As the feasibility of the output signal, using the smartphone image analysis APP developed in the group to different concentrations of TMB OX Perform image acquisition and analysis. In order to obtain different concentrations of TMB OX The color change of 500μM immune nanoflowers was diluted (1 / 2 original concentration dilution) to obtain different concentrations (2μM-500μM) of nanoflowers, and then the same volume of different concentrations of nanoflowers were used to catalyze the same volume of TMB solution , and then use the APP to collect images to obtain the gray value, and each group conducts 3 parallel experiments. Experimental results such as Figure 6 As shown, TMB OX Concentration C NF There is a good linear relationship with the gray value G. As the concentration of nanoflowers increases, the blue color deepens and the gray value decreases. The linear model can be expressed as G=-27.51×ln(C NF )+216.89(R 2 = 0.99)...

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Abstract

The invention provides a microorganism separation and detection system and a detection method. The system comprises an annular micro-fluidic chip, a rotating magnetic field device, a connecting element, a stepping motor, a stepping motor driver, a fixing device, an embedded device, smart phone image analysis equipment and the like. The microorganism separation and detection system provided by the invention integrates separation, marking, washing, catalysis and detection, has characteristics of automatic operation, high reaction speed, small size and the like, and has a wide application prospect in the aspect of rapid detection of pathogenic microorganisms such as food-borne pathogenic bacteria, animal viruses and the like.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a microorganism separation and detection system and detection method. Background technique [0002] Foodborne pathogens have always been considered the most important risk factor for food safety. Therefore, on-site screening of foodborne pathogens is of great significance to prevent foodborne disease outbreaks. [0003] At present, the commonly used isolation method of foodborne pathogenic bacteria is mainly immuno-nano-magnetic separation. However, most of these methods require manual operations. Immunomagnetic grid / net separation is a separation method that uses a special magnetic field design to make the magnetic material distributed in a chain in the channel, thereby increasing the probability of collision and combination between bacteria and magnetic materials. This separation method can effectively improve the capture efficiency and realize automatic oper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/58G01N33/535G01N21/78B01L3/00
CPCG01N33/56916G01N33/577G01N33/535G01N33/587G01N21/78B01L3/502753B01L3/5027G01N2469/10G01N2333/255B01L2200/10Y02A50/30
Inventor 林建涵霍晓婷王蕾戚武振汪思源段宏
Owner CHINA AGRI UNIV
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