A holographic optical tweezers fusion structure illumination obvious microsystem and method

Abstract

The invention provides a holographic optical tweezers fusion structure illumination obvious micro system and method, belonging to the technical field of optical microscope systems, which comprises a holographic optical tweezers micro-control system and a structured illumination obvious micro system. The holographic optical tweezers micro-control system includes A laser generating device, a light beam expanding unit and a light modulating unit, the light beam expanding unit includes a first lens and a second lens arranged in sequence along the light exit direction of the laser generating device, and the light modulating unit includes a light exit direction arranged along the light exit direction of the second lens The optical spatial light modulator and the third lens, the fourth lens, the first dichroic mirror and the objective lens arranged in sequence along the light exit direction of the optical spatial light modulator, the structured illumination microsystem includes an illumination light source and a light exit along the illumination light source A structured light generator, a second dichroic mirror, a fifth lens, a first dichroic mirror and an objective lens arranged in sequence.

Description

technical field [0001] The invention belongs to the technical field of optical microscopic systems, relates to optical microscopic imaging and optical capture technology, and in particular relates to a holographic optical tweezers fusion structure illumination obvious microsystem and method. Background technique [0002] Compared with electron microscopes, although the resolution of optical microscopes is relatively low, it is the best microscopic tool for studying living biological samples, especially in recent years, the rapid development of super-resolution optical imaging technology has increased the resolution of optical microscopes from several hundred nanometers. to a few nanometers. Therefore, optical microscope is an important tool for studying microstructure, revealing life process and diagnosing pathology in the fields of biology and medicine. At present, the mainstream super-resolution optical imaging techniques are mainly divided into two categories: one is the...

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Problems solved by technology

[0003] In view of the above-mentioned problems in the prior art that the super-resolution and three-dimensional imaging optical microscopy of biological samples in the fluid solution cannot be realized, the present invention proposes a holographic optical tweezers fusion structure illumination microscopy system and method, which is fixed by optical tweezers For particles such as cells and macromolecules swimming in the solution, the incident light wavefront is modulated by a spatial light modulator, and the expected light field is obtained in the focal area of ​​the objective lens to capture and manipulate the particles, so as to realize super-resolution and three-dimensional imaging of biological samples in fluid solutions. Microscopic imaging

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