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Detection method of yeast mitosis recombination hotspot

A technology of mitosis and detection methods, applied in the field of synthetic biology, can solve the problems of insufficient research on yeast recombination hotspots and cold spots, screening of restricted recombination strains, etc.

Active Publication Date: 2021-10-26
TIANJIN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0006] Although a lot of research has been carried out on the genomic changes of yeast mitotic recombination, due to the limitation of the construction of mitotic library and the screening of recombinant strains, the research on the hot and cold spots of yeast recombination is not enough.
However, a suitable PCRTag watermark label has not been found for the study of mitotic recombination hotspots

Method used

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  • Detection method of yeast mitosis recombination hotspot
  • Detection method of yeast mitosis recombination hotspot
  • Detection method of yeast mitosis recombination hotspot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] 1. Construction of a heavy row plant library

[0048] 1.1 strain

[0049] 1 Dalploid Saccharomycestrope YLHM120 serves as a strain, which contains a wild-type V-chromosome (WTV) and a synthetic V-chromosome (Synv) from Tianjin University.

[0050] 2 Triploid brew yeast strain YXZX1892 serve as a strain, which contains a wild-type V-chromosome (WTV) and two synthetic V-chromosome (SYNV) from Tianjin University.

[0051] 3 tetraploid brewing yeast strain YLHM086 serves as a strain, which contains two wild-type V-chromosome (WTV) and two synthetic V-chromosome (SYNV) from Tianjin University.

[0052] 1.2 induced rearrangement:

[0053] A single colonies carrying PCRE4 were picked from the SC-His plate, inoculated into 3 ml SC-His medium, oscillated at 30 ° C for 24 hours.

[0054] Centrifuge at room temperature for 2 min, and the supernatant was taken, the cells were collected and washed three times with sterile water.

[0055] The cells in step 2 were transferred to 3 ml of a...

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Abstract

The invention relates to the technical field of synthetic biology, in particular to a detection method of yeast mitosis recombination hotspot. A PCRTag tag combination provided by the invention can be used for rapidly detecting eukaryotic genome mitosis recombination hotspots. By utilizing the tag combination, the mitosis recombination hotspot of the homologous chromosome can be quickly identified through a specific watermark tag, and a convenient method is provided for researching the eukaryotic mitosis recombination event.

Description

Technical field [0001] The present invention relates to the field of synthesis biological techniques, and more particularly to detecting methods of yeast mitotic recombinant hotspots. Background technique [0002] The homologous recombination between the DNA molecules refers to a biological process between the DNA sequence undergo a double-strand break, recombinant repair, and chromosome separation process to achieve a novel DNA molecule. The homologous recombination can not only repair DNA's double-strand break (DSB), maintain genome stability, but also achieve cross-interchange of genetic information, generating genetic diversity of child cells, and accelerates biological evolution. [0003] The homologous recombination contains two ways of decancing splitting recombination and mitotic recombination, in natural state, genetic substances in cells more inclined to minor reduction splitting recombination, and the incidence of mitofer recombination is lower than the reduction split...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869C40B50/06
CPCC12Q1/6869C40B50/06
Inventor 谢泽雄元英进付娟谢心妍
Owner TIANJIN UNIV
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