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Novel tea tree endophytic biocontrol fungus Diamorpha austrialiana and application thereof

A new type of anti-fungal technology, applied in the direction of application, fungi, biocides, etc., can solve the problems of less preventive effect on tea tree diseases, late start of tea tree endophytes, and less research, so as to reduce pollution, improve antagonistic activity, and improve The effect of quality and safety

Active Publication Date: 2021-10-26
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the research on endophytic bacteria of tea tree started relatively late and there are few studies
At the same time, there are few reports on the preventive effect of using tea tree endophytic bacteria resources on tea tree diseases

Method used

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  • Novel tea tree endophytic biocontrol fungus Diamorpha austrialiana and application thereof
  • Novel tea tree endophytic biocontrol fungus Diamorpha austrialiana and application thereof
  • Novel tea tree endophytic biocontrol fungus Diamorpha austrialiana and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Isolation and identification of endophytic strains in embodiment 1

[0038] Take healthy tea tree roots, stems and leaves, wash the surface soil with tap water, soak in 70% alcohol for 1 minute, then soak in 3% sodium hypochlorite solution for 4 minutes, then soak in 70% alcohol for 1 second, and finally use sterile Rinse with water for 5 times, absorb 100uL of sterile water from the last rinse and spread it on the PDA medium to prepare the control medium.

[0039] Tissue separation method: cut the surface-sterilized tea tree tissue into small pieces of 5mm*5mm, stick the cut surface on the PDA medium, culture at a constant temperature of 25°C for 7 days, observe every day, and pick the newly grown hyphae with a transfer ring cultivated alone. figure 1 It is the effect diagram of plate culture of endophytic strains.

[0040] The ITS and 18S gene loci of the isolated endophytic fungi were sequenced, and the sequencing results were compared with BLAST on NCBI to obtain ...

Embodiment 2

[0043] Example 2 Endophyte Antagonism Experiment

[0044] In the present embodiment, the endophytic fungus Diaporthe australiana is used against 5 kinds of plant disease pathogenic bacteria (apple anthracnose pathogenic bacteria Glomerella ciningulaia (Stonem.) Spauld.et Sch.), tea wheel spot disease pathogenic Pseudopestalotiopsis theae, watermelon wilt pathogen Fusarium oxysporum f.sp. Hiveum (E.F.Smith) Wollen., tomato wilt pathogen Fusarium oxysporum f. Fusarium oxysporum f.sp.lycopersici Snyder et Hansen, tobacco black shank pathogen Phylophthora nicotianae (Phylophthora nicotianae)) plate confrontation experiment, and count the diameter of colony growth at different culture time, and calculate the inhibition bacteria rate.

[0045] The specific content is as follows:

[0046] 1. Tablet confrontation method:

[0047] Carve out a bacterium cake with a radius of 5mm from the edge of the pathogenic bacteria colony, and inoculate it on the center point of the PDA medium pl...

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Abstract

The invention belongs to the technical field of prevention and control of plant diseases and insect pests, and particularly relates to a novel tea tree endogenous biocontrol fungus Diaporthe austrialiana and application thereof. According to the application, a novel endophyte is separated from a tea tree body, the novel endophyte is identified as Diaporthe austrialiana through morphology and molecular biology, and biological activity evaluation shows that the novel endophyte has very good antagonistic and bacteriostatic activity on common pathogenic bacteria on several plants. Therefore, the strain can be used as a biocontrol bacterium of phytopathogen, and is applied to green and safe prevention and control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of plant disease and insect pest control, and in particular relates to a novel tea tree endogenous biocontrol fungus Diaporthe australiana and its application. Background technique [0002] Plant endophytes refer to microbial groups that can live in the intercellular spaces and cells of various organs and tissues of plants, and have established a harmonious symbiotic relationship with plants, including bacteria, fungi, and actinomycetes. This harmonious symbiotic relationship is manifested in that it parasitizes in the host plant during part or all of its life cycle, and is temporarily non-pathogenic to the plant. As one of the components of the plant body, endophytes play an important role in plant growth, biomass accumulation, carbon sink, bioremediation and other functions. [0003] The colonization of endophytes in host plants can promote plant growth on the one hand, and enhance the resistance of host ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCC12N1/14A01N63/30
Inventor 姜浩刘家名李佳勋黄玉琚李卓妍秦丽
Owner ANHUI AGRICULTURAL UNIVERSITY
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