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High-pressure temperature-control simulated culture device for deep-sea cold spring organisms and use method of the high-pressure temperature-control simulated culture device

A cultivation device and technology for microbial cultivation, which is applied in the field of high-pressure temperature-controlled simulation cultivation devices for deep-sea cold springs, and achieves the effects of strong corrosion resistance, low cost, and adjustable volume

Pending Publication Date: 2021-10-26
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current bottleneck problem of high-pressure large-scale organism or microorganism cultivation, the purpose of the present invention is to provide a deep-sea cold spring biological high-pressure temperature-controlled simulation cultivation device and its use method

Method used

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  • High-pressure temperature-control simulated culture device for deep-sea cold spring organisms and use method of the high-pressure temperature-control simulated culture device
  • High-pressure temperature-control simulated culture device for deep-sea cold spring organisms and use method of the high-pressure temperature-control simulated culture device

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0054] Experimental example 1 (microbial culture experiment)

[0055] Shewanella Shewanlle sp. and 2216E liquid medium are put into the microbial bag 11, the pressure in the microbial bag 11 is maintained at 1450psi after pressurization, and the microbial bag 11 is sealed and put into the hyperbaric chamber A12.

[0056] The high pressure pump A7 pumps the seawater in the mixing chamber 3 into the hyperbaric chamber A12, and the pressure (1450psi) in the hyperbaric chamber A12 can be read by the pressure gauge B15, so that the internal and external pressure of the microbial bag 11 is balanced. The temperature in the incubator A14 is kept at 2-4°C. The microorganisms in the microbial bag 11 are cultivated under pressure for 1 to 3 days. During the cultivation process, the balance cylinder 46 keeps the pressure in the microbial bag 11 at 1450 psi through the balancer 43 . After the cultivation, open the back pressure valve B29, adjust the pressure of the back pressure valve B29...

experiment example 2

[0058] Experimental Example 2 (Large Biological Culture Experiment)

[0059] First of all, large organisms refer to deep-sea invertebrates, such as (deep-sea clams, crayfish, etc.). Place large creatures in hyperbaric chamber A12. Put into the 2216E liquid culture medium that 600ml contains microorganism in the seawater cabin 56, the gas in the supplementary gas cylinder 1 is the mixed gas of 50% nitrogen, 30% methane, 20% air. The seawater with culture medium is pumped into the mixing chamber 3 through the seawater pump 55, and the supplementary gas cylinder 1 is filled with gas into the mixing chamber 3, so that the gas and the seawater with culture medium are mixed in the mixing chamber 3, and the pressure gauge A5 Read the pressure in mixing chamber 3 < 10 psi. The pressure of the mixing chamber 3 is normal pressure.

[0060] The high-pressure pump A7 pumps the mixture of gas and seawater into the hyperbaric chamber A12, and the pressure (1450psi) in the hyperbaric cham...

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Abstract

The invention belongs to the field of culture simulation experiment research on cold spring high-pressure large organisms or microorganisms, and particularly relates to a high-pressure temperature-control simulated culture device for deep sea cold spring organisms and a using method of the high-pressure temperature-control simulated culture device. The simulated device comprises a mixing cabin, a high-pressure cabin, a high-pressure filter device and various valve bodies which are connected through high-pressure pipelines. In the high-pressure cabin, a deep-sea high-temperature or low-temperature high-hydrostatic-pressure environment is realized through high-pressure pumps and a temperature control system, and besides, the culture, enrichment and fixation technology of large organisms and microorganism thalli under high pressure is realized through an enrichment filtering device and a fixing device. A culture device and the enrichment filtering device can be externally expanded, and most of materials are high in corrosion resistance, low in cost and adjustable in size.

Description

technical field [0001] The invention belongs to the research field of high-pressure large-scale organism or microorganism culture, filtration, and immobilization simulation experiments, and specifically relates to a high-pressure temperature-controlled simulation culture device for deep-sea cold spring organisms and a method for using the same. Background technique [0002] The deep sea is a typical high-pressure environment, and pressure-tolerant and pressure-loving microorganisms are important groups in the deep-sea ecosystem. With the development of deep-sea sampling technology and the development of special culture equipment for high-pressure microorganisms, a series of barophilic microorganisms have been isolated from deep-sea environments, including some strictly barophilic bacteria that cannot grow in normal pressure environments. They have gradually formed a unique mechanism different from atmospheric microorganisms in terms of fatty acid composition, pressure regula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/12C12M1/38C12M1/00
CPCC12M41/40C12M47/02C12M41/12
Inventor 连超李超伦张峘王敏晓张鑫栾振东周丽曹磊陈浩王昊钟兆山
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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