Nano-enzyme with nano-zinc oxide loading palladium nanoparticles as well as preparation method and application of nano-enzyme

A nano-zinc oxide and palladium nano-technology, applied in chemical instruments and methods, botanical equipment and methods, catalyst activation/preparation, etc., can solve problems such as inability to achieve antibacterial purposes and hazards, and improve enzyme catalytic activity and reaction process Controllable, easy-to-prepare effects

Pending Publication Date: 2021-10-15
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, high concentrations of hydrogen peroxide can cause harm to the human body, while low concentrations of hydrogen peroxide, although harmless to the human body, cannot achieve good antibacterial purposes

Method used

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  • Nano-enzyme with nano-zinc oxide loading palladium nanoparticles as well as preparation method and application of nano-enzyme
  • Nano-enzyme with nano-zinc oxide loading palladium nanoparticles as well as preparation method and application of nano-enzyme
  • Nano-enzyme with nano-zinc oxide loading palladium nanoparticles as well as preparation method and application of nano-enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] ZnO-supported palladium nanoparticles nanozyme (Pd-NPs / ZnO)

[0034] (1) preparation method comprises the steps:

[0035] 1. Put 200mg of zinc oxide (n-ZnO) nanomaterials in a round-bottomed flask containing 20mL of deionized water, and ultrasonically disperse them uniformly for about 30 minutes before use. According to the loading amount of palladium (Pd) is 0.2wt% and 0.5wt%, respectively dropwise add 0.109mL, 0.272mL palladium nitrate solution with a concentration of 8mg / mL in the solution, then add 0.3mLNa 2 CO 3 The solution was adjusted to pH=8-9. Then the mixture was heated at 90°C for 2h, cooled to room temperature, washed several times, filtered and dried in an oven at 60°C, and finally heated at 200°C under H 2 0.2wt%, 0.5wt% Pd-NPs / ZnO nanozyme can be obtained after reduction for 2h.

[0036] (2) Detection

[0037] figure 1 It is the relevant TEM image of 0.2wt% Pd-NPs / ZnO nanocomposite. Depend on figure 1 It can be seen that the palladium nanoparticl...

Embodiment 2

[0041] Mimetic peroxidase activity of nanozyme Pd-NPs / ZnO supported by palladium nanoparticles on ZnO (1) TMB experiment of n-ZnO, 0.2wt% Pd-NPs / ZnO and 0.5wt% Pd-NPs / ZnO

[0042] The simulated peroxidase activity of n-ZnO, 0.2wt% Pd-NPs / ZnO and 0.5wt% Pd-NPs / ZnO was determined by TMB experiment.

[0043] Method: Select 3,3',5,5'-tetramethylbenzidine (TMB) as the chromogenic substrate of peroxidase, take a certain amount of nanozyme and a certain concentration of H 2 o 2After the solution is mixed, it can effectively catalyze and oxidize TMB into blue oxidation state TMB (oxTMB), and there is a characteristic absorption peak at 652nm in the ultraviolet-visible absorption spectrum. By comparing the change of absorbance, it can be judged that n-ZnO, 0.2 Whether wt% Pd-NPs / ZnO and 0.5wt% Pd-NPs / ZnO nanozymes have mimic peroxidase activity.

[0044] Figure 4 It is the TMB experiment diagram of n-ZnO, 0.2wt% Pd-NPs / ZnO and 0.5wt% Pd-NPs / ZnO. Figure 4 where A and B are TMB con...

Embodiment 3

[0048] Antibacterial application of nanozyme Pd-NPs / ZnO loaded with palladium nanoparticles on ZnO

[0049] Antibacterial experiment, including the following steps:

[0050] 1) Preparation of LB medium: Weigh 8.25g of nutrient agar into an Erlenmeyer flask, add 250mL of deionized water and ultrasonically dissolve it, adjust pH=7.2-7.4 with sodium hydroxide, and obtain a solid medium. Sterilize under the conditions of 0.1MPa and 120°C, pour it into a flat plate, and place it on the upper shelf of the refrigerator for use. Weigh 0.5g of yeast extract powder, 1g of sodium chloride and 1g of tryptone into an Erlenmeyer flask, add 100mL of deionized water and ultrasonically dissolve it, and adjust the pH to 7.2-7.4 with sodium hydroxide to obtain liquid culture base, sterilized under the same conditions and placed on the upper shelf of the refrigerator for later use. All glass instruments used in the experiment were sterilized in an autoclave for use.

[0051] 2) After sterilizi...

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Abstract

The invention relates to the technical field of nano-enzyme catalytic antibiosis, in particular to application of a nano-zinc oxide loaded palladium nano composite material as nano-enzyme catalytic antibiosis. The nano-zinc oxide loaded palladium nano composite material is a nano enzyme with nano zinc oxide loading palladium nano particles, which is formed by taking granular nano zinc oxide as a carrier and palladium nano particles as an active center. The loading capacity of the palladium nanoparticles is 0.2% or 0.5%. The nano-enzyme with the nano-zinc oxide loading the palladium nanoparticles shows excellent catalytic antibacterial performance, and has potential application in the field of biomedicine.

Description

technical field [0001] The invention relates to the technical field of nanozyme-catalyzed antibacterial, in particular to the application of a nano-zinc oxide-loaded palladium nanocomposite material as nanozyme-catalyzed antibacterial. Background technique [0002] With the continuous development of nanomaterials, nanomaterials with catalytic activity and stability mimicking natural enzymes have been discovered and named as nanozymes. Nanozymes, characterized by low cost, reusability, stability, and strong catalytic activity, are considered to be powerful substitutes for natural enzymes and have aroused research interest. The reported nanozymes can be roughly divided into three categories: metal nanozymes, metal oxide or sulfide nanozymes, and carbon-based nanozymes. The main catalytic types include oxidase, peroxidase and superoxide dismutase, etc. By adjusting the size of the nanozyme, the composition and structure of the active center, etc., the catalytic activity can b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/60B01J37/03A01N59/00A01P1/00
CPCB01J23/60B01J35/023B01J37/035A01N59/00
Inventor 杨黎妮曲晓月张迪孟凡池李涛刘霄夏立新
Owner LIAONING UNIVERSITY
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