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Preparation method of micromolecular pectin

A technology for small molecules and pectin, applied in the field of preparing small molecule pectin, can solve the problems of difficult recovery of anhydrous ethanol, low yield of small molecule pectin and high cost, and achieve stable molecular weight, easy reaction control and large output. Effect

Active Publication Date: 2021-10-01
BEIJING UNIV OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of small molecule pectin is low, and the introduction of a large amount of absolute ethanol is difficult to recover and the cost is high

Method used

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  • Preparation method of micromolecular pectin
  • Preparation method of micromolecular pectin
  • Preparation method of micromolecular pectin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of small molecule pectin preparation method provided by the invention comprises the following steps successively:

[0038] (1) Dissolving pectin

[0039]Weigh 5g of high molecular weight pectin and dissolve it in 100ml of deionized water under stirring conditions, the stirring speed is 800rpm, the temperature is 60°C, and the stirring time is 60min.

[0040] (2) Enzymatic hydrolysis of pectin

[0041] After the polymer pectin is completely dissolved, pectinase whose mass is 6% of the dry weight of the polymer pectin is added, the enzymolysis temperature is 30° C., and the enzymolysis time is 60 min.

[0042] (3) Pectin oxidation

[0043] After enzymatic hydrolysis of pectin, 30% hydrogen peroxide was added immediately to make the final concentration 3%, the oxidation temperature was 30°C, and the oxidation time was 30 minutes.

[0044] (4) Rotating ceramic membrane filtration and concentration:

[0045] The small molecule pectin solution is filtered and conce...

Embodiment 2

[0050] A kind of small molecule pectin preparation method provided by the invention comprises the following steps successively:

[0051] (1) Dissolving pectin

[0052] Weigh 5g of high molecular weight pectin and dissolve it in 100ml of deionized water under stirring conditions, the stirring speed is 800rpm, the temperature is 60°C, and the stirring time is 60min.

[0053] (2) Enzymatic hydrolysis of pectin

[0054] After the polymer pectin is completely dissolved, pectinase whose mass is 7% of the dry weight of the polymer pectin is added, the enzymolysis temperature is 30° C., and the enzymolysis time is 60 minutes.

[0055] (3) Pectin oxidation

[0056] After enzymatic hydrolysis of pectin, 30% hydrogen peroxide was added immediately to make the final concentration 3%, the oxidation temperature was 30°C, and the oxidation time was 30 minutes.

[0057] (4) Rotating ceramic membrane filtration and concentration:

[0058] The small molecule pectin solution is filtered and ...

Embodiment 3

[0063] A kind of small molecule pectin preparation method provided by the invention comprises the following steps successively:

[0064] (1) Dissolving pectin

[0065] Weigh 5g of high molecular weight pectin and dissolve it in 100ml of deionized water under stirring conditions, the stirring speed is 800rpm, the temperature is 60°C, and the stirring time is 60min.

[0066] (2) Enzymatic hydrolysis of pectin

[0067] After the polymer pectin is completely dissolved, pectinase whose mass is 6% of the dry weight of the polymer pectin is added, the enzymolysis temperature is 30° C., and the enzymolysis time is 60 min.

[0068] (3) Pectin oxidation

[0069] After enzymatic hydrolysis of pectin, hydrogen peroxide was added immediately to make the final concentration 2%, the oxidation temperature was 30°C, and the oxidation time was 30 minutes.

[0070] (4) Rotating ceramic membrane filtration and concentration:

[0071] The small molecule pectin solution is filtered and concentr...

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PUM

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Abstract

The invention discloses a preparation method of micromolecular pectin. The method comprises steps of dissolving polymer pectin in deionized water to form a pectin solution, adding pectinase to carry out enzymolysis reaction, after the reaction is finished, adding hydrogen peroxide for reaction, filtering and concentrating by adopting a rotary ceramic membrane, and performing vacuum drying on the micromolecular pectin precipitate. The micromolecular pectin obtained by the method is single in molecular weight distribution; the dissolution and absorption are easier; lead ions can be effectively eliminated; meanwhile, the method is simple to operate, short in technological process, environment-friendly and easy to industrialize.

Description

technical field [0001] The invention relates to the field of high molecular compound hydrolysis, in particular to a method for preparing small molecule pectin. Background technique [0002] Pectin is a natural acidic heteropolysaccharide widely present in plant cell walls, with galacturonic acid as the main component. Its molecular weight is generally 50,000-300,000 Da, and the molecular weight obtained from different sources and extraction processes is quite different, and it has a high degree of structural diversity. In the food processing industry, pectin, as a hydrophilic colloid, has the functions of gelling, stabilizing, thickening and emulsifying. At the same time, pectin has the effects of anti-cancer, eliminating heavy metals in the body, regulating blood sugar and lowering blood lipids. However, pectin is a long-chain polysaccharide carbohydrate, which has poor water solubility, bad taste and cannot be absorbed by the human body, so its application in biological ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P19/14C08B37/00
CPCC12P19/04C12P19/14C08B37/0048Y02P20/10
Inventor 梁浩刘晓洁高肇林李慧勇张祥真
Owner BEIJING UNIV OF CHEM TECH
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