Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

IncRNA ALDB-898 related to piglet bacterial diarrhea, and application of lncRNA ALDB-898

A technology for bacterial and diarrhea, applied in the field of genetic engineering and pig disease resistance genetic breeding, can solve the problem of less mechanism of action, and achieve the effect of wide application value

Active Publication Date: 2021-09-28
GANSU AGRI UNIV
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the mechanism of action of lncRNA in piglet diarrhea caused by Clostridium perfringens type C infection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • IncRNA ALDB-898 related to piglet bacterial diarrhea, and application of lncRNA ALDB-898
  • IncRNA ALDB-898 related to piglet bacterial diarrhea, and application of lncRNA ALDB-898
  • IncRNA ALDB-898 related to piglet bacterial diarrhea, and application of lncRNA ALDB-898

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1, expression and subcellular localization of lncRNA ALDB-898

[0034] 1. Expression of LncRNA ALDB-898

[0035] 1. LncRNA transcriptome sequencing

[0036]In this study, 30 7-day-old piglets (long × large) were selected, and 5 piglets were randomly selected as the control group (IC). Bacteria oral challenge test, each piglet was fed with 1 mL of culture solution of Clostridium perfringens type C C59-2 strain, and the test period was 5 days. After the test, the scores of each defecation of each piglet during the test period were added together as the total diarrhea score of the piglet, and the total diarrhea score was sorted from high to bottom, and the top 5 piglets with the highest total diarrhea score were selected as the total diarrhea score. In the susceptible group (IS), the top 5 piglets with the lowest total diarrhea score served as the tolerant group (IR). Ileum tissue samples from groups IC, IS and IR were collected for RNA-seq. RNA-seq was complet...

Embodiment 2

[0064] Example 2, Overexpression of lncRNA ALDB-898 alleviates CPB2-induced inflammatory injury of IPEC-J2 cells

[0065] 1. Construction of LncRNA ALDB-898 overexpression vector

[0066] Step 1. Referring to the ALDB database of porcine lncRNA, use the cDNA of IPEC-J2 as a template to amplify the full-length sequence of ALDB-898. PCR reaction system (20 μL): 2×Taq PCR Master Mix 10 μL, upstream primers (10 μmol / L) 1 μL, downstream primer (10 μmol / L) 1 μL, cDNA template 2 μL, RNA free water 6 μL. PCR reaction program: pre-denaturation at 95°C for 5 min, denaturation at 94°C for 30 s, annealing at 60°C for 30 s, extension at 72°C for 60 s (31 cycles); extension at 72°C for 10 min, storage at 4°C.

[0067] The upstream primer adds a Hind III (CAAGCTT) restriction site, and its nucleotide sequence is shown in SEQ ID NO.5.

[0068] The downstream primer is added with Xba I (CTCTAGA) enzyme cutting site, and its nucleotide sequence is as shown in SEQ ID NO.6.

[0069]Step 2. Aft...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of genetic engineering and pig disease-resistant genetic breeding, and provides an lncRNA ALDB-898 sequence related to piglet bacterial diarrhea, and application of the lncRNA ALDB-898 sequence, and the nucleotide sequence of the lncRNA ALDB-898 is shown as SEQ ID NO. 1. The invention provides a fluorescent quantitative PCR detection primer pair, which is shown as SEQ ID NO.2 and SEQ ID NO.3, of the lncRNA ALDB-898, the objective existence of the lncRNA ALDB-898 is confirmed through PCR, and the lncRNA ALDB-898 is obviously reduced in the ileum of a piglet infected by C-type clostridium perfringens and in intestinal epithelial cells (IPEC-J2) treated by CPB2 toxins. An lncRNA ALDB-898 overexpression vector is constructed, a result discovers that the overexpression lncRNA ALDB-898 can remarkably inhibit IPEC-J2 cytotoxicity and inflammation induced by the CPB2 toxins, a design target is provided for research and development of novel prevention and control drugs for piglet bacterial diarrhea, and a technical foundation is laid for disease-resistant breeding of piglet C-type clostridium perfringens.

Description

technical field [0001] The invention relates to the fields of genetic engineering and pig disease-resistant genetic breeding, in particular to an lncRNA ALDB-898 related to piglet bacterial diarrhea and its application. Background technique [0002] Bacterial diarrhea in piglets is a common intestinal disease in intensive pig production, which seriously affects the healthy development and economic benefits of the pig industry. Clostridium perfringens type C, as a common pathogen, is one of the main pathogens causing diarrhea in piglets. The diarrhea caused by this bacteria is more likely to cause serious diseases due to its rapid onset and strong infectivity. CPB2 is one of the key virulence toxins produced by Clostridium perfringens type C, which can promote diarrhea and accelerate systemic infection through blood circulation. At present, the prevention and treatment of bacterial diarrhea in piglets only from the perspective of vaccines and antibiotics has limitations. Imp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/113C12N15/11C12Q1/6883C12Q1/6841C12N15/85A61K31/7105A61P1/12
CPCC12N15/113C12Q1/6883C12Q1/6841C12N15/85A61P1/12C12N2310/11C12Q2600/158C12Q2600/178C12N2800/107C12N2320/32C12Q2563/107C12Q2565/601
Inventor 高小莉滚双宝张生伟杨巧丽黄晓宇王鹏飞闫尊强
Owner GANSU AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com