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Additive for improving in-vitro maturation quality of oocytes, culture method and application

A technology for in vitro maturation and culture of oocytes, applied in the field of additives to improve the quality of in vitro maturation of oocytes, can solve the problems of lack and insufficient support for the development and growth of oocytes, and achieve the effect of promoting development

Active Publication Date: 2021-09-28
SOUTH CHINA AGRI UNIV
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Problems solved by technology

The deeper reason is that the mechanism of normal oocyte maturation in vivo is not yet clear, and the factors and substances that promote oocyte maturation in follicles have not been thoroughly studied so far, resulting in the lack of these substances in the in vitro maturation medium, which is not enough to support Better development and growth of oocytes

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  • Additive for improving in-vitro maturation quality of oocytes, culture method and application
  • Additive for improving in-vitro maturation quality of oocytes, culture method and application
  • Additive for improving in-vitro maturation quality of oocytes, culture method and application

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Embodiment Construction

[0018] Below in conjunction with embodiment the present invention is described in further detail.

[0019] 1. Quantitative proteomic detection of follicular fluid

[0020] Quantitative proteomics mass spectrometry was used to examine follicular fluid before and after in vivo maturation in order to find out which factors were significantly changed during follicle maturation. The mature follicle fluid in the ovary of the estrus sow and the immature follicle fluid in the ovary of the non-estrus sow are collected by operation. The immature follicles are follicles with a diameter of 3-8mm. Afterwards, the high-abundance protein removal kit was used to remove the high-abundance proteins in the two follicular fluids, and the samples were prepared on the machine for quantitative proteomics mass spectrometry detection. The results showed that the expression of platelet factor 4 (PF4) was significantly up-regulated , the relative expression level of PF4 protein in mature follicular flu...

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Abstract

The invention discloses an additive for improving the in-vitro maturation quality of oocytes. The in-vitro culture quality of the oocytes is improved by adding a PF4 protein additive to an in-vitro oocyte culture solution. By improving the in-vitro culture quality of the oocytes, the additive can be applied to the fields of in-vitro maturation of the oocytes, somatic cell cloning technology and parthenogenetic embryos. On one hand, in-vitro maturation of the oocytes of various species can be promoted, in-vitro fertilized embryos and cloned embryos with better quality can be prepared, and in-vitro maturation culture of the oocytes of human assisted reproduction is facilitated. On the other hand, the prepared high-quality embryos and animals produced by the embryos are used for research in the fields of medicine, life science, animal husbandry and the like, and development of production practice is further promoted.

Description

technical field [0001] The invention relates to an in vitro culture technology of oocytes, in particular to an additive for improving the quality of in vitro maturation of oocytes, a culture method and application. Background technique [0002] Oocyte in vitro maturation (in vitro maturation, IVM) technology refers to taking immature oocytes from ovarian follicles, placing them in in vitro maturation medium for culture, and simulating the environment of follicles in vivo as much as possible to make them mature And gain the ability to fertilize. This technology has very important application value in the fields of life science, medicine, human assisted reproduction and animal husbandry, and is a basic technology for obtaining a large number of mature oocytes. In the actual production of animal husbandry, mature oocytes are used as recipient cells, somatic cells of animals with excellent traits are used as donor cells, and then a large number of excellent oocytes with the sam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/075C12N15/877
CPCC12N5/0609C12N15/8778C12N2501/21C12N2501/31C12N2500/32C12N2500/84
Inventor 蔡更元梁雅琳李紫聪吴珍芳招华兴李亚楠张贤君张宇星郑恩琴杨化强洪林君顾婷徐铮黄思秀杨杰
Owner SOUTH CHINA AGRI UNIV
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