Application of Epitalon in preparation of medicine for relieving cisplatin-induced acute kidney injury

A technology of acute kidney injury and cisplatin, applied in the direction of drug combination, tetrapeptide components, urinary system diseases, etc., can solve the problems of no kidney structure and function damage

Inactive Publication Date: 2021-09-28
HANGZHOU FIRST PEOPLES HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The invention provides a use of Epitalon in the preparation of medicines for alleviating cisplatin-induced acute kidney injury. There is a technical problem that there are no suitable drugs for the treatment of cisplatin-induced renal structural and functional damage

Method used

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  • Application of Epitalon in preparation of medicine for relieving cisplatin-induced acute kidney injury
  • Application of Epitalon in preparation of medicine for relieving cisplatin-induced acute kidney injury
  • Application of Epitalon in preparation of medicine for relieving cisplatin-induced acute kidney injury

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Experimental program
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Effect test

Embodiment 1

[0018] A use of Epitalon in the preparation of a medicament for alleviating cisplatin-induced acute kidney injury-related diseases.

[0019] The Epitalon improves the cisplatin-induced acute kidney injury by up-regulating the expression of the anti-aging-related protein klotho.

[0020] 1) Materials and reagents

[0021] Epitalon was purchased from MCE Company in the United States, NGAL antibody was purchased from Abcam Company, KIM-1 antibody was purchased from R&D Systems Company, klotho and β-actin antibodies were purchased from Proteintech Company; the apoptosis detection kit was purchased from B&D Company.

[0022] 2) Cell culture and treatment

[0023] Mouse renal tubular epithelial cells (mPTCs) were cultured in DMEM / F12 medium containing 10% fetal bovine serum, 0.5% penicillin and streptomycin, and the culture conditions were 37°C, 5% carbon dioxide and 95% air.

[0024] In order to study the effect of Epitalon on the injury of renal tubular epithelial cells caused b...

Embodiment 2

[0045] Epitalon improves renal injury and renal function in a cisplatin-induced acute kidney injury model.

[0046] In order to evaluate the role of Epitalon in protecting cisplatin-induced acute kidney injury, we detected kidney-related biochemical indicators in mouse serum. After intraperitoneal injection of cisplatin for 72 hours, serum muscle and blood urea nitrogen indicators were significantly increased, and kidney Renal pathological damage such as tubule dilatation and necrosis is also relatively serious, and after Epitalon treatment, the corresponding renal damage and renal function indicators are significantly decreased, such as figure 1 A-1C.

[0047] In addition, the renal tubular injury score also suggested that Epitalon treatment could improve the renal pathological injury induced by cisplatin, as shown in Figure 1D.

[0048] Therefore, Epitalon can not only improve renal function, but also reduce renal pathological damage.

[0049] These results indicate that E...

Embodiment 3

[0053] Epitalon ameliorates cisplatin-induced acute kidney injury by upregulating the expression of klotho protein.

[0054] Further, we explored the possible mechanism of Epitalon to improve cisplatin-induced acute kidney injury. Our experimental results showed that after Epitalon treatment, the expression of klotho in mouse kidney tissue was significantly up-regulated. The results are as follows: image 3 shown.

[0055] A series of studies have shown that up-regulating the expression of klotho can improve cisplatin-induced acute kidney injury, and our results are the first to discover that Epitalon can up-regulate the expression level of klotho in mouse kidney tissue.

[0056] The above results indicated that Epitalon may improve cisplatin-induced acute kidney injury by up-regulating the expression of klotho protein.

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Abstract

The invention provides application of Epitalon in preparation of a medicine for relieving cisplatin-induced acute kidney injury, belongs to the field of biological medicine, and proves that the Epitalon improves the cisplatin-induced acute kidney injury by up-regulating expression of klotho protein in kidney tissues for the first time, so that a possibly effective prevention and treatment medicine is provided for the cisplatin-induced acute kidney injury. At present, an effective medicine for treating the acute kidney injury caused by the cis-platinum is still lacked clinically, so that it is possible for the invention to provide the effective clinical medicine for preventing and treating the acute kidney injury caused by the cis-platinum.

Description

technical field [0001] The present invention relates to a new use of Epitalon, in particular to a use of Epitalon in the preparation of medicaments for alleviating cisplatin-induced acute kidney injury. Background technique [0002] In the 1970s, cisplatin began to be widely used clinically to treat a variety of malignant tumors, including bladder cancer, cervical cancer, head and neck cancer, and small cell or non-small cell lung pain. It is one of the most effective and commonly used chemotherapeutic drugs for tumors. However, while cisplatin has a killing effect on tumor cells, it will cause a series of serious adverse reactions to normal tissues and organs, which greatly limits its clinical application. The adverse reactions of cisplatin mainly include nephrotoxicity and other systemic organ damage reactions, among which nephrotoxicity is the most common. After treatment with cisplatin, about 1 / 3 of the patients developed renal dysfunction leading to acute renal failure....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/07A61P13/12
CPCA61K38/07A61P13/12
Inventor 王龙黄进宇梁平莫绪明郭晓令郑洁王帅高贝贝林晓晓杨运文刘益海孔凡奇黄佳敏董晓华王万田
Owner HANGZHOU FIRST PEOPLES HOSPITAL
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