Application of glucogalactooligosaccharide in preparation of medicine for treating and/or preventing ischemia-reperfusion injury
A technology of galacto-oligosaccharides and ischemia-reperfusion, which is applied in drug combinations, pharmaceutical formulas, cardiovascular system diseases, etc., to achieve the goal of reducing cognitive dysfunction, strong drug efficacy, and reducing renal ischemia-reperfusion injury Effect
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Embodiment 1
[0041] Protective effect of glucogalactooligosaccharides on hepatic ischemia-reperfusion injury
[0042] 1. Establishment of animal models
[0043] The experimental animals are male C57 / BL6J mice, 6-8 weeks old, 22-26g. Raised under standard experimental conditions, 12-hour light-12-hour dark cycle, free access to water and food. The mice were anesthetized with isoflurane, and the abdomen was operated on, and the blood supply of the middle lobe and left lobe of the liver was blocked with a non-traumatic hemostatic clip, resulting in 70% liver ischemia. After 30 min of ischemia, the clip was removed, and then reperfused After 6 hours, samples were taken to measure biochemical indicators.
[0044] 2. Experimental grouping
[0045]By intraperitoneal injection, the mice were randomly divided into four groups, the first group was the sham operation group (sham), the animals in the sham operation group received the same laparotomy, but no vascular clip was blocked; the second gro...
Embodiment 2
[0056] Protective effect of glucogalactooligosaccharides on renal ischemia-reperfusion injury
[0057] 1. Establishment of animal models
[0058] The experimental animals were male C57 / BL6J mice, 6-8 weeks old, 22-26g. The mice were anesthetized with isoflurane, and the abdomen was operated on. Under sterile conditions, a 1.5 cm longitudinal incision was made along the double dorsal side to enter the abdominal cavity, the muscles were bluntly separated, and the renal pedicles on both sides were exposed. After 30 minutes of ischemia, the arterial clamp was released, and the abdominal cavity was closed after the renal perfusion resumed. After 24 hours, samples were taken to measure biochemical indicators, so as to establish the RIRI model.
[0059] 2. Experimental grouping
[0060] By intraperitoneal injection, the mice were randomly divided into four groups, the first group was the sham operation group (sham), the animals in the sham operation group received the same laparoto...
Embodiment 3
[0071] The protective effects of glucogalactooligosaccharides against cerebral ischemia-reperfusion injury.
[0072] 1. Establishment of animal models
[0073] The experimental animals are male C57 / BL6J mice, 6-8 weeks old, 22-26g. Anesthetize mice with isoflurane and place them supine on a heating pad. The skin was disinfected with iodine and 75% ethanol. A midline neck incision was made to expose the carotid artery (MCA). The external carotid artery (ECA) was then isolated and occluded with two knots. Next, the internal carotid artery (ICA) was isolated, and the carotid artery (MCA) and internal carotid artery (ICA) were clamped with microvascular clamps. A small hole was cut in the external carotid artery (ECA) between the junction and the bifurcation. Then, the MCAO suture with a length of 30 mm and a diameter of 0.11 mm coated with silicone rubber was introduced into the internal carotid artery (ICA). While sutures are inserted to block the blood vessel, the artery ...
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