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Liquid chromatography-tandem mass spectrometry determination method of fluensulfone metabolite

A fluthiazide sulfone and tandem mass spectrometry technology, applied in the field of pesticide residue detection, can solve problems such as alveolar/bronchiole hyperplasia and lung cell proliferation

Active Publication Date: 2021-09-21
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Toxicological data show that fluthiazol can cause cell proliferation in the lungs of mice, and further lead to alveolar / bronchiole hyperplasia, eventually leading to the appearance of tumors
And studies have shown that BSA metabolites have similar toxicity to fluthiazol

Method used

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  • Liquid chromatography-tandem mass spectrometry determination method of fluensulfone metabolite
  • Liquid chromatography-tandem mass spectrometry determination method of fluensulfone metabolite
  • Liquid chromatography-tandem mass spectrometry determination method of fluensulfone metabolite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The QuEChERS extraction method of residual fluthiazol metabolites in tobacco of the present embodiment comprises the following steps:

[0027] Weigh 2.00g (accurate to 0.01g) of tobacco dust sample into a 50mL capped centrifuge tube, add 10mL of water, shake manually until the sample is fully soaked in water, and then let stand for 10min. Add 10mL of acetonitrile, vortex at 2000r / min for 2min, freeze at -18°C for 10min, add 4g of anhydrous magnesium sulfate, 1g of sodium chloride, 1g of sodium citrate and 0.5g of disodium citrate, and immediately Vortex and oscillate for 2 minutes to prevent local overheating and agglomeration caused by the reaction of anhydrous magnesium sulfate with water, then centrifuge at 4000r / min for 10min, take 1mL supernatant into a 1.5mL centrifuge tube, add 150mg anhydrous magnesium sulfate and 25mgN -Propylethylenediamine bonded solid-phase adsorbent, vortexed at 2000r / min for 2min, then centrifuged at 4000r / min for 10min, the supernatant wa...

experiment example 2

[0045] The recovery experiment was carried out on the blank tobacco powder sample at three levels of 10, 20, and 50 mg / kg, and the relative recovery rate was in the range of 95.6-102.9%. The lowest concentration standard working solution matched by the blank matrix is ​​gradually diluted with the blank matrix, and the detection limit is 3 times the signal-to-noise ratio, and the quantification limit is 10 times the signal-to-noise ratio. The detection limit of this method is: 0.01 mg / kg, Limit of quantification: 0.03 mg / kg.

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Abstract

The invention relates to a liquid chromatography-tandem mass spectrometry determination method of fluensulfone metabolite, and belongs to the technical field of pesticide residue detection. The method comprises the following steps: extracting a sample with an organic solvent by adopting a QuEChERS pretreatment method, centrifuging, carrying out dispersive solid-phase extraction and purification on supernate, and determining by using liquid chromatography-tandem mass spectrometry. The liquid chromatography-tandem mass spectrometry analysis method of the fluensulfone metabolite is established for the first time, and is suitable for extraction and determination of fluensulfone metabolite residues in samples such as vegetables, melons and fruits, tobaccos and the like. When the residual quantity of the fluensulfone metabolite is measured, a blank sample of the fluensulfone metabolite is adopted for matrix standard matching, so that the measurement result is more accurate.

Description

technical field [0001] The invention relates to a method for determining residual fluthiazol metabolites in tobacco by liquid chromatography tandem mass spectrometry, and belongs to the technical field of pesticide residue detection. Background technique [0002] Fluthiazide sulfone, fluoxene sulfone, and flusulfone sulfone are fluoroolefin sulfide compounds. They are the only new chemical nematicides developed in the past 20 years. They have contact activity and can make growers get rid of Reliance on fumigant nematicides while avoiding some of the adverse consequences of fumigant application. Fluthiazol metabolites can be used for the comprehensive control of nematode diseases in various crops, such as eggplant, pepper, tomato, tobacco and other solanaceous crops, cucumber, zucchini, pumpkin, watermelon, cantaloupe and other melon crops, Compositae, cruciferous Leafy vegetables, potatoes, sweet potatoes and other yams. [0003] Fluthiazol was developed by Israel Maxim Co...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634G01N2030/047
Inventor 邓惠敏杨飞范子彦刘珊珊纪元王颖边照阳唐纲岭
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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