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Mouse esophageal squamous carcinoma cell line as well as establishment method and application thereof

A technology for esophageal squamous cell carcinoma and establishment methods, which is applied in the direction of tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the problems of unstable cell lines, low purity of tumor cells, long-term culture, etc., and achieve simple operation and high purity High, good tumorigenic effect

Inactive Publication Date: 2021-09-21
SUN YAT SEN UNIV CANCER CENT
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  • Abstract
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  • Claims
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Problems solved by technology

However, the established cell line may not be stable. It has only been passed on for five generations and cannot be cultured in vitro for a long time. This may be due to the low purity of tumor cells. It is also difficult to carry out related in vitro experiments, and the operation steps of its establishment method are relatively cumbersome.

Method used

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  • Mouse esophageal squamous carcinoma cell line as well as establishment method and application thereof
  • Mouse esophageal squamous carcinoma cell line as well as establishment method and application thereof
  • Mouse esophageal squamous carcinoma cell line as well as establishment method and application thereof

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Embodiment

[0031] A method for the establishment of a mouse-derived esophageal squamous cell carcinoma stable cell line

[0032] 1. Establishment of primary esophageal squamous cell carcinoma model in mice

[0033] 1) Prepare 5 mg / mL 4-nitroquinoline-N-oxo stock solution with propylene glycol and store at 4°C;

[0034] 2) Dilute the above 4-nitroquinoline-N-oxo stock solution in the drinking water of mice, and the final concentration obtained by dilution is 100 μg / mL, and replace it once a week. After 12 weeks of continuous feeding, replace it with a drug-free Ordinary drinking water, continue feeding for 12 weeks;

[0035] 3) After 24 weeks, the mice were sacrificed, and the tumor tissue was stripped, such as figure 1 As shown, there are obvious tumors in the mouse esophagus;

[0036] 4) A part of the tumor tissue was taken and fixed in neutral formalin liquid, then dehydrated by machine, embedded in paraffin, and sectioned for HE staining or immunohistochemical detection;

[0037] ...

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Abstract

The invention belongs to the technical field of tumor cell lines, and particularly relates to a mouse esophageal squamous carcinoma cell line as well as an establishment method and application thereof. The method comprises the following steps: 1) establishing a mouse orthotopic esophageal squamous cell carcinoma model: inducing cancerization of esophageal mucosa cells of a mouse by adopting a chemical reagent; 2) culturing a mouse esophageal squamous carcinoma cell line: cleaning the tumor tissue with a serum-free medium, cutting the tumor tissue into blocks, placing the blocks in a DMEM culture medium containing 20% FBS, and culturing at constant temperature; and continuously culturing and cleaning tissue blocks with cells climbing out, adding pancreatin for digestion, centrifuging, taking supernatant, re-suspending the cell precipitate, re-paving the cell precipitate in a culture bottle, and culturing to obtain the mouse esophageal squamous cell carcinoma stable cell line. According to the method, the mouse esophageal squamous carcinoma cell line capable of being stably passaged can be obtained within a short time, the tumor formation property is good, the method is a method for rapidly constructing a mouse esophageal squamous carcinoma cell model with a sound immune microenvironment, and the obtained cell line is used for in-vitro stable culture and can be repeatedly used for multiple times.

Description

technical field [0001] The invention belongs to the technical field of tumor cell lines, and in particular relates to a mouse esophageal squamous cell line and its establishment method and application. Background technique [0002] Esophageal cancer is one of the most common malignant tumors, and its morbidity and mortality rank sixth among all cancers in the world. my country is a country with a high incidence of esophageal cancer, and more than half of the world's cases occur in my country. In China, the most common histological type of esophageal cancer is squamous cell carcinoma. Surgery is an important treatment for resectable esophageal squamous cell carcinoma. For advanced cases, postoperative adjuvant therapy is also considered necessary. In addition, neoadjuvant chemoradiotherapy has been proven to have a significant effect on improving the survival of patients with esophageal squamous cell carcinoma. However, postoperative recurrence and metastasis are still dif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02C12R1/91
CPCC12N5/0693G01N33/5005
Inventor 文静方烁桂谢绚谢秀英傅剑华
Owner SUN YAT SEN UNIV CANCER CENT
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