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A kind of plant source compound bacteriostatic agent

A plant source and bacteriostatic agent technology, which is applied in pharmaceutical formulas, cosmetic preparations, dressing preparations, etc., can solve the problem of low antibacterial activity, achieve strong antibacterial effect, improve antibacterial ability, and broad antibacterial spectrum Effect

Active Publication Date: 2022-06-07
广州萃普生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the inventors have found in the process of screening a large number of natural plants for antibacterial activity that some literatures have reported the antibacterial activity of Zanthoxylum bungeanum and peony extracts, but when using Zanthoxylum bungeanum or peony alone, their antibacterial activity is still lower than The chemical antibacterial and parabens that are often used in the market, therefore, the inventor further optimized the extraction process of Zanthoxylum bungeanum and peony, studied the reasonable compound ratio of Zanthoxylum bungeanum and peony, and compared the antiseptic effect of commonly used chemical cosmetic preservatives A comparison was made and a preliminary study was made on the mechanism of their synergistic anticorrosion

Method used

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  • A kind of plant source compound bacteriostatic agent
  • A kind of plant source compound bacteriostatic agent
  • A kind of plant source compound bacteriostatic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation method of peony flower extract:

[0024] Take 40g of peony flowers to dry, grind the fine powder in a 1000mL round bottom flask, add 20 times the volume of 70% ethanol as the extraction solvent, use an electric heating sleeve equipped with a cold flow reflux device, at 60 °C, reflux extraction for 2h, vacuum filtration to collect filtrate, repeat 2 times. The filtrate was concentrated by rotational evaporation in varnish at 40 °C, and stood for standing, and the supernatant was taken to freeze-dried to obtain peony flower extract lyophilized powder.

Embodiment 2

[0025] Example 2: Preparation method of pepper fruit extract:

[0026] Take 25kg of peppercorns, dry the powder at 60 °C, heat the reflux extraction with 95% ethanol for 3 times, each time for 2h, combine the filtrate after filtration, concentrate the filtrate under reduced pressure to extract 600g, add water and suspend it with petroleum ether, dichloromethane, ethyl acetate, n-butanol extract, concentrate under reduced pressure, and obtain n-butanol extract 340g; The n-butanol extract was dissolved with methanol, ethanol, and water, filtered, volatilized and dried into a viscous liquid, sampled onto a macroporous resin that had been eluted with distilled water, enriched with active ingredients, first eluted with an ethanol-water system (0:100~15:85~30:70~50:50~75:25~95:0) for gradient elution, and then used water-methanol system (100:0~90:10~60:40~40:60~0:100) gradient elution, combined eluent, decompression concentration, Spray drying is provided.

Embodiment 3

[0027] Example 3: Determination of bacteriostatic effect (sheet diffusion method):

[0028] The principle of the paper diffusion method: the paper piece containing the quantitative bacteriostatic substance is attached to the agar plate that has been inoculated and measured, and the bacteriostatic substance contained in the paper piece continuously spreads to the area around the paper sheet to form a decreasing gradient concentration. The growth of the test bacteria is inhibited within the range of bacteriostatic concentrations around the paper sheet, thus forming a transparent bacteriostatic circle. By measuring the diameter of the bacteriostatic circle, the strength of the bacteriostatic substance can be judged, and the larger the diameter of the bacteriostatic circle, the stronger the bacteriostatic effect of the drug.

[0029]First, according to the nutrients required by the test bacteria, the corresponding solid medium is prepared, the medium melted and cooled to 45-50 ° C is ...

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Abstract

The invention discloses a compound bacteriostatic agent of plant origin. The bacteriostatic agent is compounded by peony flower extract and Zanthoxylum bungeanum extract according to mass percentage, wherein the content of peony flower extract is 20%-50%. Zanthoxylum bungeanum extract content is 50%‑80%. The plant source compound antibacterial agent of the present invention has a wide antibacterial spectrum and strong antibacterial effect, and has a wide antibacterial spectrum against Gram-positive bacteria, Gram-negative bacteria, yeasts and molds, and fully embodies the complex The synergistic effect of the complex overcomes the limitation of the antibacterial effect of a single plant extract. The antiseptic ability of the plant-derived compound bacteriostatic agent is basically equivalent to that of the chemical bacteriostatic agent propylparaben, and can replace the use of traditional chemical antiseptics. Experiments preliminarily show that the plant-derived compound antibacterial agent acts on different parts of the bacterial cell wall, cell membrane, nucleic acid, and enzyme protein, and synergizes to improve the antibacterial ability of the composition.

Description

Technical field [0001] The present invention relates to the field of cosmetic natural antiseptic technology, in particular to a potent, broad-spectrum bacteriostatic activity plant source compound bacteriostatic agent. Background [0002] To prevent microbial contamination of cosmetics and ensure the quality and safety of cosmetics, the most effective way at present is to add preservatives. However, the vast majority of cosmetic preservatives that are currently allowed to be used are chemical synthetic products, such as parabens, methylisothiazolinone, phenoxyethanol, etc., but the presence of different degrees of irritation and biological toxicity, its potential safety risks have aroused widespread concern in the industry, antiseptic substitution has become a hot topic of research, more and more scholars study preservatives of natural origin, of which the antiseptic function of plant extracts has attracted much attention. The research on natural preservatives in cosmetics in Chi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61Q19/00
CPCA61K8/9789A61Q19/00A61K2800/524A61K2800/5922A61K2800/10Y02A50/30
Inventor 涂禄清邓建科
Owner 广州萃普生物科技有限公司
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