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A strain of Glomus spp

A technology of balloon mold and saline-alkali land, which is applied in the field of saline-alkali land restoration, saline-alkali land restoration composition, and surface balloon mold.

Active Publication Date: 2022-07-08
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high-salt and high-alkaline environment in saline-alkali land is not conducive to the survival of microorganisms and restoration plants, which seriously restricts the application of microorganism-plant joint restoration of saline-alkali land.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Morphological and Molecular Identification of Surface Glomus

[0027] The Glomus versiforme of the present invention is preserved in the General Microorganism Culture Collection Center of China Microorganism Culture Collection Management Committee, and the deposit number is CGMCC No.14546.

[0028] The spores of the present invention have the following morphological characteristics: the spores are round or nearly round, yellow to yellow-brown, with a dark circle along the spore wall under reflected light, and the spore diameter is 90-150 μm. The spore wall is three-layered, 4-6 μm thick, the first layer is colorless and transparent perishable wall, about 1 μm, which often falls off when mature, and sometimes only part of the debris remains. The second layer is pale yellow to pale yellow-brown, with lamellar walls, 3-4 μm. The third layer is single-walled, 1 μm, and the second layer cannot be divided, and the spores are visible after rupture, which is bright golden yell...

Embodiment 2

[0031] Effects of Glomerulus terrestriali on the root infection rate of pioneer plants under different salinity gradients

[0032] Test scheme: The infection rate of S. chinensis roots by G. serrata under the conditions of salinity of 1‰, 3‰, 5‰ and 8‰ was investigated respectively. The specific steps are as follows: after the soil is sterilized by gamma rays, put it into a commercial 410*310*145mm turnover box, each turnover box is filled with 15kg of sterilized soil, the soil salinity is adjusted with sodium chloride, and the box is inoculated every week 2,000 spores of G. globosa on the surface; sown sphaerenoides seeds, and keep 8 s. sylvatica strains in the box every week. After 4 months of culture in the greenhouse, the infection rate was determined by the improved triphenyl blue staining method. The test results are shown in Table 1.

[0033] Table 1 The infection rate of G. terrestris on the root system of S. chinensis under different salinity gradients

[0034] ...

Embodiment 3

[0037] The effect of Glomerulus terrestris on the preservation rate of pioneer plants under different salinity gradients

[0038] Test scheme: The preservation rate of S. chinensis on S. chinensis was investigated under the conditions of salinity of 1‰, 3‰, 5‰ and 8‰, respectively. The specific steps are as follows: After the soil is sterilized by gamma rays, it is put into a commercial 410*310*145mm turnover box, and each turnover box is filled with 15kg of sterilized soil, and the soil salinity is adjusted with sodium chloride, and each salinity is 6 3 pots were inoculated with 2,000 spores of G. globulus on the surface, and the other 3 pots were not inoculated; 30 saplings were planted in each pot, cultivated for 4 months, and the number of surviving ss. .

[0039] Table 2 The effect of inoculation with G. terrestris under different salinity on the preservation rate of S. chinensis

[0040] salinity 1‰ 3‰ 5‰ 8‰ not vaccinated 100％ 100％ 80％ 40％ i...

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Abstract

The invention provides a strain of Glomus spp., a composition for restoring saline-alkali land, and a method for restoring saline-alkali land, belonging to the technical field of ecological restoration. The surface Glomus fungus provided by the present invention has a preservation number of CGMCC No. 14546, has good root infection ability for pioneer plants in saline-alkali soil with 8 / 1000th salt content, can significantly improve the survival rate of pioneer plants in saline-alkali land, and improves soil Repair effect.

Description

technical field [0001] The invention belongs to the technical field of ecological restoration, and in particular relates to a surface Glomus fungus, a composition for restoring saline-alkali land and a method for restoring saline-alkali land. Background technique [0002] Saline-alkali soil resources are a very important reserve arable land resource in China. my country's saline-alkali soils are mainly distributed in the plains of 23 provinces, municipalities and autonomous regions in 5 regions, including the coastal saline-alkali soil area, the Huang-Huai-Hai Plain saline-alkali soil area, and the northwest half of the Arid saline-alkali soil area and dry saline-alkali soil area and northeast saline-alkali soil area. [0003] Among many saline-alkali soil improvement measures, water conservancy engineering measures, agricultural farming measures, chemical amendments and other methods have been widely used, but the effect is not satisfactory. Although chemical improvement me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C09K17/14C12R1/645C09K101/00C09K109/00
CPCC12N1/14C09K17/14C09K2101/00C09K2109/00C12N1/145C12R2001/645B09C1/105
Inventor 张倩邢锦程杨海水孙启祥褚建民郭嘉张明乾
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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