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Method for characterizing short-cut nitrification and denitrification stability of sewage based on SOUR online detection

A short-range nitrification and denitrification technology, applied in chemical instruments and methods, test water, biological water/sewage treatment, etc., can solve problems such as changes in the performance of sewage treatment processes

Pending Publication Date: 2021-09-10
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, the composition and activity of microorganisms are easily affected by various factors such as water inflow, operating parameters, and environmental conditions, which in turn cause changes in the performance of sewage treatment processes.

Method used

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  • Method for characterizing short-cut nitrification and denitrification stability of sewage based on SOUR online detection
  • Method for characterizing short-cut nitrification and denitrification stability of sewage based on SOUR online detection
  • Method for characterizing short-cut nitrification and denitrification stability of sewage based on SOUR online detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1SOUR on-line continuous detection characterizes microbial activity

[0066] 1. Use SBR reactor to treat synthetic wastewater, and add culture medium to the reactor to obtain synthetic wastewater. Specifically: In the SBR reactor, add medium and activated sludge, the concentration of activated sludge is 3500-6000mg / L; the formula of the medium is: ammonia nitrogen 30mg / L, COD 210mg / L, inorganic salt medium. The way of adding the culture medium is: slowly adding the culture medium during the water inlet process in each reaction cycle of each stage, so, after each cycle of each stage, when entering the next cycle, the feed water in the reaction chamber The water is synthetic waste water (medium will be added).

[0067] Wherein, the formula of inorganic salt medium is: Na 2 HPO 4 12H 2 O 1000mg / L, KH 2 PO 4 1000mg / L, (NH 4 ) 2 SO 4 300mg / L, MgSO 4 ·7H 2 O 250mg / L, trace elements 5.0mL / L.

[0068] The formula of trace elements is: EDTA 0.5g / L, ZnSO ...

Embodiment 2A

[0082] The SOUR detection of embodiment 2AOB, NOB

[0083] In the SBR reactor, the aerobic operation is about 30 minutes, and the mud-water mixture in the SBR reactor is pumped into the breathing chamber. The volume of the breathing chamber is 2L. At this time, close the two-way ball valve on the overflow pipe to prevent the mud-water mixture from flowing to In the reaction chamber; turn on the stirring motor and the aeration pump, aerate until the DO rises to 8mg / L, stop the aeration and seal it, and automatically add sodium chlorate solution when the DO drops to 6mg / L to make the concentration in the mixed solution be 0.02mol / L; when DO drops to 4mg / L, allylthiourea is automatically added to make the concentration in the mixture 20mg / L, and the measurement is continued until DO drops to 0mg / L. This process is an intermittent online detection. During this process, the breathing chamber is aerated until the DO reaches mg / L and stops. The timing of the detection is: during the...

Embodiment 3

[0087] Embodiment 3 Ammonia oxidation, nitrite oxidation rate detection

[0088] The activated sludge samples in the whole stage, commissioning stage and short stage of the SBR reactor were taken, washed three times with distilled water, and shake flask experiments were used to investigate the ammonia oxidation rate and nitrite oxidation rate of the active samples in each stage. The amount of activated sludge inoculum at different stages was 20%, the shaking table speed was controlled at 120rpm, and the temperature was 25°C. Water samples were taken for 0h, 1h, and 3h to detect the concentration of ammonia nitrogen and nitrite. The results are shown in Table 5 below.

[0089] Table 5 Ammonia nitrogen concentration and nitrite concentration in different time periods

[0090]

[0091] As shown in the table, during the full nitrification stage, the ammonia oxidation rate (SAOR) and nitrite oxidation rate (SNOR) of the activated sludge were 1.37mg / L / h and 1.80mg / L / h respective...

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Abstract

The invention relates to the technical field of microbial activity detection, in particular to a method for characterizing short-cut nitrification and denitrification stability of sewage based on SOUR online detection. According to the specific technical scheme, the method for characterizing the short-cut nitrification and denitrification stability of the sewage based on SOUR online detection is characterized in that operation parameters of an SBR reactor in a whole-process nitrification and denitrification stage and operation parameters of the SBR reactor in a debugging stage and a short-cut nitrification and denitrification stable operation stage are regulated and controlled, and the specific oxygen uptake rate and the specific value of different nitrifying bacteria are controlled. According to the method for characterizing the short-cut nitrification and denitrification stability of the sewage based on SOUR online detection, real-time online detection is carried out on the SOUR change rule in the whole process from the whole process to the short-cut process, the SOUR change rules under different denitrification conditions are determined, the SOUR response rule under the stable operation condition of the short-cut nitrification and denitrification process is investigated, and the metabolic activity of nitrifying microorganisms in the short-cut nitrification and denitrification process can be effectively reflected.

Description

technical field [0001] The invention relates to the technical field of microbial activity detection, in particular to a method for characterizing short-range nitrification and denitrification stability of sewage based on SOUR online detection. Background technique [0002] Biological method is widely used in sewage treatment due to its advantages of good purification effect, low cost and no secondary pollution. At present, most of the sewage treatment biological treatment systems in my country adopt the traditional denitrification process, but this process has the disadvantages of high aeration energy consumption, low denitrification efficiency, and long treatment time during operation. In recent years, short-cut nitrification and denitrification, as a new type of denitrification method, can reduce the aeration rate by 25% and reduce the carbon source by 40% compared with the whole-process denitrification process, which can shorten the reaction time and reduce the sludge pro...

Claims

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Application Information

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IPC IPC(8): C02F3/30G01N33/18
CPCC02F3/302G01N33/188
Inventor 谭周亮陈杨武周后珍李欣官悦朱荣霞
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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