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Novel semi-quinone free radical nanometer material and preparation method and application thereof

A technology of nanomaterials and free radicals, applied in biochemical equipment and methods, pharmaceutical formulations, genetic engineering, etc., can solve problems such as difficult application and complex preparation of free radicals, and achieve simple and feasible methods, eliminating purification steps and maintenance costs low effect

Active Publication Date: 2021-09-07
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Finally, a one-step method is realized to prepare multifunctional nanomaterials with both peroxidase-like activity and persistent free radical properties, so as to make up for the complex preparation and difficult application of free radicals in the prior art

Method used

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  • Novel semi-quinone free radical nanometer material and preparation method and application thereof
  • Novel semi-quinone free radical nanometer material and preparation method and application thereof
  • Novel semi-quinone free radical nanometer material and preparation method and application thereof

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Effect test

Embodiment 1

[0043] Novel semiquinone radical nanomaterials with persistent radical and peroxidase activity

[0044] The G-quadruplex is a special nucleic acid secondary structure formed by the folding of DNA or RNA containing continuous G sequences under specific conditions. Its four G bases are connected by Hoogsteen hydrogen bonds to form a G-tetrad planar structure, and then adjacent G-tetrads form four stable G-quadruplexes through π-π stacking. Heme can be loaded into the G-tetrad interlayer space via π-π stacking to form a DNase with peroxidase activity.

[0045] During the oxidation process of dopamine, it will generate semiquinone free radical intermediates, unpaired electrons in the outermost electron orbit of free radical electrons, resulting in short life and instability. The G-tetrad containing the planar nitrogen heterocycle of guanosine can act as the π electron acceptor, and the oligomer containing the semiquinone free radical acts as the π electron donor, and the two can ...

Embodiment 2

[0048] Preparation

[0049] 1) Annealing and renaturation of nucleic acid containing continuous G sequence to obtain an aqueous solution containing G-quadruplex, adding heme to the aqueous solution and incubating at room temperature for 20-40min to obtain a G4 / Hemin complex, the G-quadruplex The molar ratio of the body to the hemoglobin is 1:1 to 1:200;

[0050] 2) Stir and mix the G4 / Hemin complex and dopamine aqueous solution, and use Tris to adjust the pH to 7-10, and the mass ratio of the G-quadruplex to the dopamine is 0.01:1-2:1;

[0051] 3) The mixture obtained in step 2) is further stirred and centrifuged to obtain the semiquinone radical nanomaterial; the stirring time is 10-30 hours; the centrifugal speed is 5000-20000.

[0052] Morphological characterization (TEM, AFM) of the nanomaterials, such as image 3 , Figure 4 As shown, the size is 1-30 μm, and the thickness is about 15 nm. The composite nanosheets were analyzed by ESR, and the spectra are as attached ...

Embodiment 3

[0054] Efficacy verification

[0055] Since the semiquinone free radical nanomaterials prepared by the present invention can generate ·OH by using oxygen, the nanosheets are dispersed in water containing oxygen for a period of time, centrifuged and detected ·OH in the supernatant aqueous solution. Using TEMPO as a free radical scavenger, the spectrum of ESR analysis is as follows Image 6 , indicating that the composite nanosheets can utilize oxygen in water to generate OH. In addition, terephthalic acid, a specific fluorescent probe for ·OH, was used to detect the amount of ·OH produced by nanosheets with different concentrations. The result is as Figure 7 As shown, as the concentration of semiquinone radical nanosheets increases, the fluorescence intensity increases. In addition, if Figure 8 As shown, the ability of the nanosheets synthesized for different days to produce OH gradually decreased, and 50% efficiency was still maintained after 60 days, indicating that the...

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Abstract

The invention belongs to the field of nano material synthesis, and particularly relates to a novel semi-quinone free radical nano material as well as a preparation method and an application thereof. According to the semi-quinone free radical nano material, a G4 / Hemin compound formed by a G-quadruplex structure folded by a guanine-rich nucleic acid sequence and heme is used as a basic unit, and the basic unit is used as a pi electron acceptor; dopamine forms a semi-quinone free radical intermediate in the oxidation process to provide a pi electron donor, and the pi electron donor and the pi electron acceptor are subjected to a pi-pi accumulation effect to form a stable free radical; the rest dopamine is further oxidized, so that the G-quadruplex structure and the semi-quinone unit are subjected to cross-linking polymerization.

Description

technical field [0001] The invention belongs to the field of nanomaterial synthesis, and in particular relates to a novel semiquinone free radical nanomaterial and its preparation method and application. Background technique [0002] Organic free radicals possess unpaired electrons and exhibit unique responses to various environmental factors, showing great potential in the field of life sciences. However, organic free radicals are unstable and easily gain and lose electrons and lose redox activity and paramagnetic properties. Studies have pointed out that the stability adjustment of organic radicals includes steric hindrance protection, adjustment of electron spin resonance density, and introduction of heteroatoms (Chem. Sci. 2020, 11, 1192-1204). Based on this, scientists have prepared a series of stable organic free radical small molecules, realizing the synthesis and purification of organic free radicals (Eur. J. Med. Chem. 2017, 138, 590-601; Molecules 2016, 21, 841). ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G73/06A61L15/42A61L15/46A61L15/26C12N15/113
CPCC08G73/0672A61L15/42A61L15/46A61L15/26C12N15/113A61L2400/12A61L2300/404A61L2300/216C12N2310/12C08L79/04
Inventor 张吉喜王振强陈榆桦谢西月贾瑞瑞
Owner CHONGQING UNIV
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