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Chaetomium globosum HJF 13 strain and application thereof

A technology of Chaetomium globosum and strains, applied in the field of applied microbiology, can solve the problems of allergy, skin redness and the like, and achieve the effects of low cost of raw materials, simple production process and good inhibitory activity

Pending Publication Date: 2021-09-03
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, more and more daily chemical products have added high nutritional ingredients such as collagen, hyaluronic acid, and peptides. While protecting the skin, they also create more convenient conditions for the reproduction of microorganisms. Consumers use microorganisms exceeding the standard Cosmetics can cause skin redness, allergies and other adverse reactions. Therefore, it is urgent to find an efficient and low-toxic biological control method to reduce the pollution of products in some special fields by Bacillus subtilis
In the previous research, the inventor screened a strain of Chaetomium cochliodes HJF 13 (Chaetomium cochliodes HJF 13) which has a good inhibitory effect on Bacillus subtilis from the endophytic fungus isolated from the Polygonatum plant, which can be used for the biological control of Bacillus subtilis. Prevention and treatment, through literature search, no relevant literature reports

Method used

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  • Chaetomium globosum HJF 13 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Identification of Chaetomium cochliodes HJF 13 (Chaetomium cochliodes HJF 13)

[0023] The DNA of the strain was extracted by the conventional CTAB method, and the ITS sequence was amplified by fungal universal primers ITS1 and ITS4. The PCR product was sent to Beijing Qingke (Kunming) Xinye Biotechnology Co., Ltd. for sequencing, and the sequenced DNA sequence was stored in the NCBI database. Carrying out BLAST search comparison, the ITS sequence of bacterial strain is 100% similarity with Chaetomium cochliodes HMAS:244354 effective published species, therefore, bacterial strain is named as Chaetomium cochliodes HJF 13 (Chaetomium cochliodes HJF 13), this bacterial strain has It was deposited in the China Center for Type Culture Collection on April 9, 2021, address: Wuhan University, Wuhan, China, and the collection number is CCTCC NO: M2021343.

Embodiment 2

[0024] Embodiment two: the preparation method of microbial anti-bacillus subtilis agent

[0025] 1. Test tube culture

[0026] The strain HJF 13 was inoculated on the solid medium slant of the test tube, and cultured at 28°C for 6 days to obtain the test tube species. The solid medium is the commonly used PDA medium. The method is to peel 200g of potatoes and cut them into small pieces, add 1000ml of water to boil for 20 minutes and then filter. Bacteria for 30 minutes.

[0027] 2. Liquid seed culture

[0028] Adopt 500ml Erlenmeyer flask shake flask culture, the method is to prepare liquid seed culture medium earlier, and liquid seed culture medium is the PDB culture medium commonly used, and method is: the potato 200g is peeled and cut into fritters, adds water 1000ml and boils for 20 minutes and filters, obtains Glucose 20g was added to the filtrate, and water was added to make up to 1000ml, and 100ml liquid seed culture medium was filled in each Erlenmeyer flask, and st...

Embodiment 3

[0031] Embodiment three: plate antibacterial test

[0032] 1. Microbial anti-Bacillus subtilis agent for preparation test

[0033] The microbial anti-bacillus subtilis agent of the present invention is prepared from Chaetomium cochliodes HJF 13 (Chaetomium cochliodes HJF 13) bacterial strain through a liquid fermentation method. The specific preparation process and conditions are the same as those described in the summary of the invention.

[0034] 2. Cultivation of Bacillus subtilis

[0035] Inoculate the Bacillus subtilis on the slant medium of the test tube and cultivate it at 25°C for 5-6 days, and wait for the bacteria to grow well for later use. The medium is conventional ISP2 medium, and its formula is: yeast powder 4g; glucose 4g; malt extract 10g; water 1000ml, add agar powder 15g to the medium, and sterilize at 121°C for 30 minutes.

[0036] 3. Plate antibacterial test method

[0037] Take the cultured Bacillus subtilis 0.5cm 2 Mix the slant of the large test tu...

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Abstract

The invention provides chaetomium globosum and application thereof, and belongs to the technical field of applied microbiology. The chaetomium globosum (chaetomium cochliodes) HJF 13 strain disclosed by the invention is preserved in the China Center for Type Culture Collection on April 9, 2021, and the preservation number of the chaetomium globosum HJF 13 strain is CCTCC NO: M2021343. An anti-bacillus subtilis agent prepared from the strain through liquid fermentation culture has a relatively good inhibition effect on bacillus subtilis and can be used for preventing and treating the bacillus subtilis. The invention has the advantages of high efficiency, low toxicity, low raw material cost and simple production process, and has potential and practical application value in the fields of daily chemical products, food, industrial products and the like.

Description

technical field [0001] The invention relates to a chaetomium cochliodes HJF 13 and its application in preparing an anti-bacillus subtilis agent, which belongs to the technical field of applied microbiology. Background technique [0002] Chaetomium globosa belongs to the genus Chaetomium. As a kind of effective biocontrol fungi, it can produce antagonistic effects on various pathogenic bacteria. In addition to reducing the incidence of plant diseases such as tomato wilt caused by Fusarium and apple spot caused by black star fungus, Chaetomium globosa can also reduce the incidence of Candida albicans, Escherichia coli, Bacillus subtilis, Pseudomonas fluorescens and other pathogenic bacteria have good inhibitory activity, and have broad application prospects in the research and development of medicine and pesticides. [0003] Bacillus subtilis is a Gram-positive bacterium with a fast growth and reproduction speed. Although Bacillus subtilis has good application value in the bi...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P1/00A23L3/3571C12R1/645
CPCC12N1/14A01N63/30A23L3/3571
Inventor 李晚谊李智敏左智天王元忠张庭张霁赵艳丽
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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