Preparation method of bioactive substance coated polyester reticular lamellar stent

Abstract

The invention provides a preparation method of a bioactive substance coated polyester reticular lamellar stent. The preparation method comprises the following steps: reinforcing a polyester material into a reticular lamellar layer through spun bonding; cleaning the reticular lamellar layer, and then drying the cleaned reticular lamellar layer; performing carboxylation treatment on the cleaned and dried reticular lamellar layer by using a strong alkaline solution; activating and cross-linking the reticular lamellar layer; coupling the reticular lamellar layer for multiple times to form a bioactive substance coating layer on the reticular lamellar layer; and sealing the coated reticular lamellar layer to obtain the bioactive substance coated polyester reticular lamellar stent. The polyester material is spun-bonded and processed into the reticular lamellar layer, the reticular lamellar layer is subjected to four steps of carboxylation, activation and crosslinking, coupling and sealing to achieve coating, covalent connection of a bionic extracellular matrix and a synthetic macromolecular polyester material is achieved by adjusting parameters, special equipment such as a plasma surface treatment machine is not needed, and toxic chemical substances are not introduced, so that the safety quality of biological product production is ensured.

Description

technical field [0001] The present application relates to the technical field of tissue engineering, in particular to a method for preparing a biologically active substance-coated polyester mesh sheet scaffold. Background technique [0002] Physiological activities such as cell proliferation, differentiation, and metabolism are seriously affected by the regulation of the microenvironment. A bioreactor is a bioreactor for large-scale production of cells or for large-scale production of cells or for harvesting cell culture products. Among them, anchorage-dependent cells mainly add scaffold materials to the reactor to provide a cell growth surface, and realize high-density cell culture through the high specific surface area of ​​the material. [0003] At present, commonly used scaffold materials in bioreactors mainly include Cytodex microcarriers, Cytopore microcarriers, and fibrous scaffolds. The Cytodex microcarrier as a cell surface attachment matrix is ​​a spherical two-di...

AI Technical Summary

Problems solved by technology

The reactor culture mode based on Cytopore microcarrier is stirring type. Although this porous structure can reduce the damage of shear force to cells to a certain extent, it cannot fundamentally eliminate the influence of shear force on cells.

Fibrous scaffolds such as FibraDisk carriers have certain mechanical strength and good mechanical properties, and can realize the three-dimensional culture structure of cells in vitro. However, the biocompatibility of pure polymer materials still has a certain gap compared with natural extracellular matrix components. defect

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