Preparation method and application of fibronectin-functional cell composite type microencapsulation transplant
A technology of fibronectin and microencapsulation, applied in the field of biomedicine, can solve the problems of short survival period of functional cells, poor long-term treatment effect, shortage of active functional cells, etc., shorten the time of cell confluence and eliminate the risk of heterogeneous infection , Promote the effect of body cell and tissue regeneration
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Embodiment 1
[0038] Example 1 Preparation method of "fibronectin and bovine cardiomyocyte composite microencapsulated graft":
[0039] 1. Selection of bovine cardiomyocyte donors: SPF newborn cattle were selected as cardiomyocyte donors.
[0040] 2. Separation, purification and identification of bovine cardiomyocytes: Dissect myocardial tissue from SPF newborn cattle and place it in a sterile plate, add an appropriate amount of pre-cooled PBS, wash away blood clots and blood cells, remove blood vessels, fat, and connective tissue, and then Rinse 2-3 times with pre-cooled PBS, transfer to scintillation vials filled with pre-cooled PBS, cut myocardial tissue into 1mm 2 Evenly sized pieces. Add 0.12% type Ⅰ collagenase, add magnetic particles to the scintillation vial, and digest in a water bath at 37 °C at 250 r / min for 10 min. Discard the supernatant after the first digestion, add new digestive enzymes to continue digestion for 10 min, and repeat digestion 4-5 times until the tissue turns...
Embodiment 2
[0043] Example 2 Preparation method of "Fibronectin and Porcine Islet Cell Composite Microencapsulated Transplant":
[0044] 1. Porcine islet isolation: dissect the pancreas from SPF pigs under clean conditions, place the pancreas on an ultra-clean workbench, quickly remove peripancreatic fat, blood vessels and pancreatic capsule, retain the intrinsic capsule, wash with UW solution and weigh on a balance . Transect the pancreas from the junction of the head and tail of the pancreas, find the main pancreatic duct, insert a plastic tube, and perfuse a collagenase solution (1.5 g / L, containing DNase 0.3 g / L). Digest in a water bath at 37°C for 30 minutes. During the digestion process, the pH is about 7.5. From 20 minutes of digestion, samples are taken every 4 minutes. Dithizone staining is used for microscopic examination. When the pancreatic tissue is digested and cracked into fine sand, the microscopic examination shows that most of the structures are completed. The islets ...
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