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A kind of msnas-tpp multifunctional nanoparticle and its preparation method and application

A nanoparticle and multi-functional technology, applied in the field of medicine, can solve the problems of lack of tumor mitochondria-specific targeted therapy, low delivery efficiency of effective means of nanoparticles, etc. Effect

Active Publication Date: 2022-07-08
ZHEJIANG UNIV HANGZHOU GLOBAL SCI & TECH INNOVATION CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the embodiment of the present application is to provide a MSNAs-TPP multifunctional nanoparticle and its preparation method and application, so as to solve the problems of lack of effective means for tumor mitochondria-specific targeted therapy and low transport efficiency of nanoparticles in related technologies

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  • A kind of msnas-tpp multifunctional nanoparticle and its preparation method and application
  • A kind of msnas-tpp multifunctional nanoparticle and its preparation method and application
  • A kind of msnas-tpp multifunctional nanoparticle and its preparation method and application

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preparation example Construction

[0056] An embodiment of the present invention provides a method for preparing MSNAs-TPP multifunctional nanoparticles, comprising: mixing SiO 2 Nanoparticles were dispersed in water to obtain solution A; solution A was mixed with water and absolute ethanol, then potassium permanganate was added, and then the solution was transferred to a hydrothermal reactor for reaction; after the reaction, cooled to room temperature, and then centrifuged to collect MSNAs Nanoparticles; MSNAs nanoparticles were added to polyethyleneimine solution for amination modification to obtain MSNAs-PEI as solution B; triphenylphosphine bromide and 1-(3-dimethylaminopropyl)-3 - Ethylcarbodiimide hydrochloride was dissolved in an aqueous solution of dimethyl sulfoxide to obtain solution C; n-hydroxysuccinimide was added to solution C and stirred at room temperature to form TPP activation solution D; The solution D was added to the solution B, and stirred, centrifuged, and washed at room temperature to ob...

Embodiment 1

[0064] This example provides a preparation method of MSNAs-TPP multifunctional nanoparticles, and the method may include the following steps:

[0065] Step (1), the SiO 2 The nanoparticles are dispersed in water to obtain solution A;

[0066] Specifically, the SiO 2 Nanoparticles can use traditional prepared by the method. Further, 1.0 g of aqueous ammonia solution was weighed and added to a mixed solution containing 30 mL of absolute ethanol and 500 μL of water, and then 1.5 mL of TEOS was added dropwise to the above solution. After stirring at room temperature for 4 h, the SiO was collected by centrifugation at 12,000 rpm for 10 min. 2 Nanoparticles, washed several times with ethanol and water, and then redispersed in 10 mL of water to give solution A at 60 mg / mL.

[0067] Step (2), mix solution A, water and absolute ethanol, then add potassium permanganate, then the solution is transferred to the hydrothermal reactor for reaction;

[0068] Specifically, 2 mL of 60 mg...

Embodiment 2

[0080] This example provides a preparation method of MSNAs-TPP multifunctional nanoparticles, and the method may include the following steps:

[0081] Step (1), the SiO 2 The nanoparticles are dispersed in water to obtain solution A;

[0082] Specifically, the SiO 2 Nanoparticles can use traditional prepared by the method. Further, 1.5 g of aqueous ammonia solution was weighed and added to a mixed solution containing 30 mL of absolute ethanol and 500 μL of water, and then 1.0 mL of TEOS was added dropwise to the above solution. After stirring at room temperature for 4 h, the SiO was collected by centrifugation at 12,000 rpm for 10 min. 2 Nanoparticles, washed several times with ethanol and water, and then redispersed in 10 mL of water to give solution A at 40 mg / mL.

[0083] Step (2), mix solution A, water and absolute ethanol, then add potassium permanganate, then the solution is transferred to the hydrothermal reactor for reaction;

[0084] Specifically, 2 mL of 40 mg...

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Abstract

The invention discloses a MSNAs-TPP multifunctional nanoparticle, a preparation method and application thereof. The preparation method comprises: mixing SiO 2 Nanoparticles were dispersed in water to obtain solution A; solution A was mixed with water and absolute ethanol, then potassium permanganate was added, and then the solution was transferred to a hydrothermal reactor for reaction; after the reaction, cooled to room temperature, and then centrifuged to collect MSNAs Nanoparticles; adding MSNAs nanoparticles to polyethyleneimine solution for amination modification to obtain MSNAs-PEI, as solution B; triphenylphosphine bromide and 1-(3-dimethylaminopropyl)-3 Ethylcarbodiimide hydrochloride is dissolved in an aqueous solution of dimethyl sulfoxide to obtain solution C; n-hydroxysuccinimide is added to solution C, and stirred at room temperature to form TPP activation solution D; The solution D was added to the solution B, and stirred, centrifuged, and washed at room temperature to obtain MSNAs-TPP multifunctional nanoparticles.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an MSNAs-TPP multifunctional nanoparticle and a preparation method and application thereof. Background technique [0002] Despite advances in the treatment of tumors, cancer remains one of the leading causes of death worldwide. Traditional treatments, such as surgery, radiotherapy, and chemotherapy, have limited efficacy and have serious side effects. Over the past few decades, numerous functional nanomaterials have been developed for tumor microenvironment-responsive drug delivery and specific cancer therapy. However, the tumor microenvironment is highly heterogeneous, both between patients and within individual tumors. Therefore, it is difficult to completely eliminate tumors by simply relying on the targeting of the tumor microenvironment. [0003] Mitochondria are the most important organelles in the cell, responsible for the energy supply of the cell and many other essential cellu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/54A61K47/59A61K33/32A61K49/18A61P35/00C01B33/20B82Y5/00
CPCA61K47/54A61K47/59A61K33/32A61P35/00A61K49/1833A61K49/1857B82Y5/00C01B33/20
Inventor 李翔谢丛坤傅译可
Owner ZHEJIANG UNIV HANGZHOU GLOBAL SCI & TECH INNOVATION CENT
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