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Efficient artificial seedling raising method for culturing Amomum villosum by using basal stem cluster buds and 'node propagation'

A Yangchunsha, high-efficiency technology, applied in the biological field, can solve the problems of low level of overall application of tissue culture seedlings and failure to form complete plants, and achieve the effects of improving seedling quality, easy standardization, and saving land resources

Active Publication Date: 2021-08-31
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are only very few reports on tissue culture in Yangchunsha, all of which adopt the method of explant-callus-adventitious buds, but only stay at the successful induction of callus, and do not further form a complete plant, making tissue culture seedlings Overall adoption is still low

Method used

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  • Efficient artificial seedling raising method for culturing Amomum villosum by using basal stem cluster buds and 'node propagation'
  • Efficient artificial seedling raising method for culturing Amomum villosum by using basal stem cluster buds and 'node propagation'
  • Efficient artificial seedling raising method for culturing Amomum villosum by using basal stem cluster buds and 'node propagation'

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Experimental program
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Effect test

Embodiment 1

[0069] A kind of high-efficiency artificial seedling raising method that Yangchunsha utilizes basal stem clustering buds and "node propagation" cultivation, comprising the following steps:

[0070] (1) Acquisition of explants: select robust plants with good growth potential and no pests and diseases, and get their underground rhizome buds.

[0071] (2) Wash the rhizome buds of step (1) with tap water to remove the surface soil, then soak them with 10% washing powder solution by mass percentage for 10 minutes, shake and stir slightly, then rinse them with running water for 30 minutes, and place them on the ultra-clean workbench with a volume percentage of 75%. % ethanol solution for 15 seconds, then sterilized with 0.1% mercuric chloride aqueous solution for 10 minutes, and finally rinsed with sterile water for 3 times, each time not less than 3 minutes, and fully shake the vessel during the whole disinfection process.

[0072] (3) Start culture and preliminary multiplication: ...

Embodiment 2

[0086] A kind of high-efficiency artificial seedling raising method that Yangchunsha utilizes basal stem clustering buds and "node propagation" cultivation, comprising the following steps:

[0087] (1) Acquisition of explants: select robust plants with good growth potential and no pests and diseases, and get their underground rhizome buds.

[0088] (2) Wash the rhizome buds of step (1) with tap water to remove the surface soil, then soak them with 10% washing powder solution by mass percentage for 10 minutes, shake and stir slightly, then rinse them with running water for 30 minutes, and place them on the ultra-clean workbench with a volume percentage of 75%. % ethanol solution for 15 seconds, then sterilized with 0.1% mercuric chloride aqueous solution for 10 minutes, and finally rinsed with sterile water for 3 times, each time not less than 3 minutes, and fully shake the vessel during the whole disinfection process.

[0089] (3) Start culture and preliminary multiplication: ...

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Abstract

The invention provides an efficient artificial seedling raising method for culturing Amomum villosum by utilizing basal stem cluster buds and 'node propagation', which comprises the following steps: inoculating a culture medium with disinfected and sterilized rhizome explant with buds, and carrying out basal stem cluster bud generation and proliferation, 'node propagation' proliferation, rooting induction and acclimatization and transplantation. The production process of the Amomum villosum seedlings is optimized and adjusted, basal stem clumpy bud induction generation, proliferation and 'node propagation' proliferation are synchronously carried out, the tissue culture process is simplified, the three culture processes are simultaneously carried out in one culture medium, and the artificial in-vitro propagation efficiency is greatly improved. In addition, the phenomena of low multiplication coefficient, low hereditary stability and the like in the tissue culture process of zingiberaceae cardamom plants are solved, and the method is low in cost, short in period and high in quality and survival rate.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-efficiency artificial seedling raising method of Yangchunsha using basal shoot clustered buds and "node propagation" cultivation. Background technique [0002] Amomum is the herb of Zingiberaceae (Zingiberaceae) Amomum (A.villosum Lour.), green shell sand (A.villosum Lour.var xanthioides T.L.Wu et Senjen) or Hainan sand (A.longiligulare T.L.Wu) The dry and mature fruit of the Chinese herbal medicine, which is pungent and warm in nature, has the effects of dispelling dampness and appetizing, warming the spleen and stopping diarrhea, regulating qi and calming the fetus. main drug. Modern pharmacological studies have shown that Amomum contains volatile components such as bornyl acetate, camphor, and borneol, which have pharmacological properties such as gastrointestinal protection, analgesia, anti-inflammatory, antidiarrheal, antibacterial, regulating flora, hypoglyc...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008Y02P60/14
Inventor 孟庆红董鲜徐福荣李宏哲黄衡宇麦湘
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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