Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection method of multi-drug-resistant microorganisms

A detection method and multi-drug resistance technology, applied in microorganism-based methods, microorganism determination/inspection, microorganisms, etc., can solve the problems of inaccurate interpretation of results, low sensitivity, and no background, so as to reduce the background signal intensity and improve the The effect of detection specificity and improved detection sensitivity

Active Publication Date: 2021-08-27
CHONGQING UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a detection method for multidrug-resistant microorganisms, to achieve the purpose of background-free, rapid and ultra-sensitive detection, and to solve the problem of long detection period, low sensitivity, background signal in detection results, and problems caused by traditional detection techniques. Key technical problems such as inaccurate interpretation of results due to human subjective factors, and serious non-specific amplification in isothermal amplification reactions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of multi-drug-resistant microorganisms
  • Detection method of multi-drug-resistant microorganisms
  • Detection method of multi-drug-resistant microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Detection method of methylene oxygen-resistant Staphylococcus aureus

[0059] This embodiment includes the following steps in turn:

[0060] S1. Conversion of protein signals

[0061] 1 Wenye MRSA (Normalized Methylene Golden Staphylococcus) adapter 100 μl and 10 μm of Blocker solution 100 μl, 10 min in a water bath at 100 ° C, naturally cooled to 20 ° C, resulting in a mixed product A 1 ,spare;

[0062] 2 will mix the product a 1 Add to 600 μl of concentration of 10 mg / ml of a three-iron oxide magnetic bead solution, then add 5 μl of an EDC solution having a concentration of 10 mg / ml, incubate at 15 ° C for 20 h, resulting in a suitable functionalized magnetic bead B. 1 ;

[0063] 3 Remove the genus, add the adapter functionalized magnetic bead B 1 In the case of 50 ° C, the rotation speed is 20 rpm, after the replacement reaction is 30 minutes, the magnetic bead is absorbed with the magnet, take the supernatant, to obtain the blocker solution y. 1 ;

[0064]...

Embodiment 2

[0078] Example 2 Method for detecting a multi-drug-resistant copper green pseudoma

[0079] S1. Conversion of protein signals

[0080] 1 Mix 10 μm of the multi-drug-resistant copper green psesson (MDR-PA) adapter solution 100 μl and 10 μm of Blocker solution 100 μl, reacted at 90 ° C for 3 min, naturally cooled to 30 ° C, resulting in a mixed product A 2 ,spare;

[0081] 2 will mix the product a 2 The addition of 550 μl of the secondary ferric magnetic bead solution was added, and the NHS solution having a concentration of 10 mg / ml was added, and 10 h was incubated under 35 ° C for 10 h, resulting in the adapter functionalized magnetic bead B. 2 ;

[0082] 3 Remove the genus, add the adapter functionalized magnetic bead B 2 In the case of 20 ° C, the rotation speed is 100 rpm, after 30 min, the magnetic bead is absorbed by the magnet, and the supernatant is taken, and the blocker solution is 2 ;

[0083] S2. Synthesis of single layer graphene

[0084]200 ml of mixed acid was pre...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a detection method of multi-drug-resistant microorganisms, the detection method comprises the following steps: capturing the multi-drug-resistant microorganisms by using nucleic acid aptamer magnetic beads, releasing a blocking sequence complementary with an aptamer, converting a protein signal into a nucleic acid signal, amplifying the blocking sequence through an index amplification reaction, and measuring the amplified blocking sequence. According to the invention, a molecular enhancer is added into an index amplification reaction system to inhibit non-specific amplification reaction, and a nanoscale transition metal dihalogen compound is added as a result reading carrier in the determination process, so that the intensity of a background signal is further reduced. The method is simple, convenient and efficient to operate, can achieve the purpose of background-free, rapid and hypersensitive detection, and can solve the key technical problems that a traditional detection technology is long in detection period and low in sensitivity, a background signal exists in a detection result, result interpretation is inaccurate due to the influence of human subjective factors, and severe non-specific amplification exists in isothermal amplification reaction. The method is suitable for detecting the drug resistance of the microorganisms.

Description

Technical field [0001] The present invention belongs to the technical field of multi-drug resistance bacterial protein detection, and is related to a drug resistant microorganism, specifically a method of detecting multiple resistance microorganisms. Background technique [0002] The magnetic bead is a magnetic nanoparticle, which has a wide range of applications in the separation and detection of pathogenic bacteria due to its advantage of having a large surface area, easy to modify, and convenient. By modifying functional groups on the surface of the magnetic beads, such as streptavidin, carboxy, etc., biomolecules such as nucleic acids, antibodies can be attached to the surface of the magnetic beads to form functional magnetic beads. Among them, the Aptamer-Functionalized MagneTic Beads, AFMBs is characterized by high, easy to separation, and is widely concerned. [0003] A low poly DNA or RNA of nucleic acid fit, is screened by an index enrichment method, which has strong bin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/682C12Q1/689C12Q1/14C12Q1/04C12R1/445C12R1/45C12R1/385C12R1/01
CPCC12Q1/682C12Q1/689C12Q2600/106C12Q2600/16C12Q2525/205C12Q2531/119C12Q2537/143C12Q2563/107Y02A50/30
Inventor 胡孝林罗阳张亮亮陈恒屹于兴乐刘睿宁
Owner CHONGQING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com