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Extraction method and application of asiatic acid

An extraction method and a technology for asiatic acid, applied in the field of extraction of asiatic acid, can solve problems such as alcoholic hepatitis of asiatic acid that have not been found yet, and achieve the effects of high purity, high yield and clear chemical structure

Pending Publication Date: 2021-08-27
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of Asiatic acid in Potentilla japonicus in the clinical treatment of alcoholic hepatitis.

Method used

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  • Extraction method and application of asiatic acid
  • Extraction method and application of asiatic acid
  • Extraction method and application of asiatic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 asiatic acid

[0037] 1) After fully pulverizing 10 kg of dried Potentilla spinach, add 80 L of 75% ethanol aqueous solution, heat and reflux for extraction 3 times, filter and combine the ethanol extracts for 3 times, recover ethanol in a vacuum, and obtain crude ethanol extract.

[0038] 2) The ethanol crude extract is extracted successively with petroleum ether, chloroform, and ethyl acetate, and the volumes of the three are respectively 1 / 3, 3 / 3, and 4 / 3 of the volume of the ethanol crude extract, and each extraction is performed 3 times until Until the ethyl acetate solution was colorless and transparent, the ethyl acetate extract was collected, concentrated and dried to obtain the ethyl acetate extract.

[0039]3) Dissolve the ethyl acetate extract in methanol, and filter through a microporous membrane (0.45 μm); the filtrate is concentrated to obtain a powder, and then recrystallized with methanol to obtain a fraction; then put it ...

Embodiment 2

[0041] The preparation of embodiment 2 asiatic acid

[0042] 1) After fully pulverizing 10 kg of dried Potentilla spinach, add 80 L of 70% ethanol aqueous solution, heat and reflux for extraction 3 times, filter and combine the 3 ethanol extracts, recover ethanol in a vacuum, and obtain crude ethanol extract.

[0043] 2) The ethanol crude extract is extracted successively with petroleum ether, chloroform, and ethyl acetate, and the volumes of the three are respectively 1 / 4, 4 / 3, and 3 / 2 of the volume of the ethanol crude extract, and each extraction is performed 4 times until Until the ethyl acetate solution was colorless and transparent, the ethyl acetate extract was collected, concentrated and dried to obtain the ethyl acetate extract.

[0044] 3) Dissolve the ethyl acetate extract in methanol, and filter through a microporous membrane (0.22 μm); the filtrate is concentrated to obtain a powder, and then recrystallized with methanol to obtain a fraction; then put it on a sili...

Embodiment 3

[0046] The preparation of embodiment 3 asiatic acid

[0047] 1) After fully crushing 10 kg of dried Potentilla spinach, add 80 L of 80% ethanol aqueous solution, heat and reflux for extraction 3 times, filter and combine the ethanol extracts for 3 times, recover ethanol in a vacuum, and obtain crude ethanol extract.

[0048] 2) The ethanol crude extract is extracted successively with petroleum ether, chloroform, and ethyl acetate, and the volumes of the three are respectively 1 / 2, 2 / 3, and 5 / 3 of the volume of the ethanol crude extract, and each extraction is performed twice until Until the ethyl acetate solution was colorless and transparent, the ethyl acetate extract was collected, concentrated and dried to obtain the ethyl acetate extract.

[0049] 3) Dissolve the ethyl acetate extract in methanol, and filter through a microporous membrane (0.45 μm); the filtrate is concentrated to obtain a powder, and then recrystallized with methanol to obtain a fraction; then put it on a...

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Abstract

The invention discloses an extraction method and application of asiatic acid. The method comprises the following steps: 1) heating, refluxing and extracting potentilla chinensis by using an ethanol solution, and filtering to recover ethanol to obtain an ethanol crude extract; sequentially extracting the ethanol crude extract by using petroleum ether, chloroform and ethyl acetate, concentrating and drying to obtain an ethyl acetate extract; and 2) dissolving the ethyl acetate extract in methanol, filtering with a microfiltration membrane, concentrating the filtrate, recrystallizing the obtained powder with methanol, loading the obtained fraction on a silica gel column, carrying out gradient elution and thin-layer chromatography to collect the eluent of the same component, loading the sixth fraction on the silica gel column, carrying out gradient elution and thin-layer chromatography to collect the eluent of the same component to obtain four sub-fractions, and recrystallizing the third sub-fraction by using methanol. The application refers to application of asiatic acid in preparation of drugs for preventing and treating alcoholic hepatitis. The invention provides a novel compound with a clear structure and high efficiency for treatment of alcoholic hepatitis, and develops a new medication approach.

Description

technical field [0001] The invention relates to the technical field of natural extracts, in particular to an extraction method and application of asiatic acid. Background technique [0002] The traditional Chinese medicine Potentilla Chinesis is a dried whole herb of the genus Potentilla Chinesis in the family Rosaceae, also known as Potentilla Chinesis, Pulsatilla Pulsatillae, Toad Grass, and Tianqingdibai. Asiatic acid is a triterpene acid compound that can be isolated from Potentilla. At present, the raw materials for the preparation of Asiatic acid are mostly Centella asiatica. For example, patent documents CN101991624B, CN106349320B, and CN109320580A all relate to the process of extracting Asiatic acid from Centella asiatica. At present, although there have been related reports on obtaining Asiatic acid from the traditional Chinese medicine Potentilla, the specific process conditions have not been made public. [0003] Alcoholic hepatitis (AH), one of the alcoholic l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61K31/56A61K36/73A61P1/16A61P29/00
CPCC07J63/008A61K31/56A61K36/73A61P1/16A61P29/00A61K2236/333A61K2236/53A61K2236/55
Inventor 韦锦斌陈思韵黄瑀莘苏宏梅朱武嫦韦园园孔令军何赟
Owner GUANGXI MEDICAL UNIVERSITY
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