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Application of Bacillus amyloliquefaciens in preventing diarrhea of ​​weaned piglets

A technology for dissolving Bacillus amyloliquefaciens, applied in the field of microorganisms, can solve the problems of no research on separating Bacillus amyloliquefaciens, etc., and achieve the effects of improving the body's antioxidant capacity, reducing the rate of diarrhea, and increasing daily weight gain

Active Publication Date: 2022-05-13
广州百仕肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have found that Bacillus amyloliquefaciens can be isolated from the intestines of some specific animals, such as CN112239736A "Bacillus amyloliquefaciens, bacterial agent, screening method and application", the Bacillus amyloliquefaciens is derived from the Obtained from the intestinal tract of crayfish; CN103966118A "Bacillus amyloliquefaciens and its application", the Bacillus amyloliquefaciens is isolated from the digestive tract of flounder fish, but there is no report on the application of Bacillus amyloliquefaciens to pig breeding , and there is no study to isolate Bacillus amyloliquefaciens suitable for pig breeding

Method used

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  • Application of Bacillus amyloliquefaciens in preventing diarrhea of ​​weaned piglets
  • Application of Bacillus amyloliquefaciens in preventing diarrhea of ​​weaned piglets
  • Application of Bacillus amyloliquefaciens in preventing diarrhea of ​​weaned piglets

Examples

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Embodiment 1

[0027] Example 1 Isolation and identification of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain BA40

[0028] 1) Screening of Bacillus strains

[0029] Take 1g of jejunum, ileum intestinal content and feces samples from 180-day-old Jinhua pigs, dilute them with sterilized normal saline at a ratio of 1:10, bathe in 75°C water for 15 minutes, and then dilute them in a 10-fold gradient with sterile water. -3 、10 -4 、10 -5 、10 -6 For four dilution gradients, draw 100 μL of the bacterial suspension and spread it on the LB plate, cool the upper LB semi-solid medium to about 50°C, and add Escherichia coli (ATCC 25922) and Salmonella (ATCC 14028 ), Staphylococcus aureus (ATCC 25923), Clostridium perfringens (ATCC 13124), make at least 3 plates for each dilution gradient and each pathogenic bacterium, pick the strains with the ability to inhibit the indicator bacteria, and perform morphological According to the scientific observation, the strains with the characteri...

Embodiment 2

[0039] Example 2 Feeding experiment to prevent weaned piglets from diarrhea

[0040] Prepare the fresh bacterial liquid of Bacillus amyloliquefaciens BA40, streak the above-mentioned Bacillus amyloliquefaciens BA40 on the solid medium LB, take a single colony and inoculate it in the LB liquid medium, and cultivate it in a shaker at 37°C for 12 hours, which is the seed The obtained seed liquid was transferred to fresh LB liquid medium at a volume ratio of 1:100, and cultured at 37°C and 120-200rpm for 24 hours to obtain the required fresh bacterial liquid.

[0041]Feeding experiment: 12 Landrace dual-element sows were selected for the experiment, and were randomly divided into 3 groups. The probiotic BA40 group was fed with BA40 fresh bacterial solution every day from 5d to 24d (10 8 CFU / mL) 1mL, the probiotic product group selects commercially available probiotic products (mixture products of Lactobacillus plantarum, Pediococcus pentosaceae and Bifidobacterium), and feeds pigl...

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Abstract

The invention relates to a strain of Bacillus amyloliquefaciens and its application in preventing diarrhea of ​​weaned piglets. The present invention provides a Bacillus amyloliquefaciens strain BA40, which can inhibit common porcine enteropathogens Escherichia coli K88, Salmonella typhimurium and Staphylococcus aureus, and has acid resistance, bile salt resistance and pancreatin resistance Ability. After the bacillus amyloliquefaciens is activated, its number of live bacteria reaches 10 8 -10 9 CFU / mL, oral gavage of 6-day-old suckling piglets, continuous gavage of 1 mL until weaning on 24 days. The Bacillus amyloliquefaciens provided by the invention can increase the weaning weight gain of piglets and the diversity of intestinal flora, reduce diarrhea in weaned piglets, reduce the rate of diarrhea, reduce inflammatory factors, promote nutrient absorption, and improve the body's antioxidant capacity ability and disease resistance, providing a new way for nutritional regulation in non-antibiotic farming.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of a strain of Bacillus amyloliquefaciens in preventing diarrhea of ​​weaned piglets. Background technique [0002] Animal husbandry is a pillar industry in my country's agricultural economic industry. For a long time in the past, antibiotics have also played an important role in promoting the development of animal husbandry: promoting growth, increasing feed remuneration, reducing morbidity, and bringing substantial benefits to producers. On the other hand, antibiotics also bring food safety and ecological safety problems, such as causing the imbalance of intestinal flora in animals, inhibiting the function of intestinal barrier, and residual antibiotics reaching the human body through the food chain, which may seriously damage Human health, while causing a series of problems such as the deterioration of the land environment. [0003] Pig breeding is the la...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
CPCA61K35/742A61K9/0053A61P1/12A61P1/00A61P39/06A61P31/04
Inventor 汪以真姜子鹏何欢张钰李文涛文超越都帅靳明亮路则庆王凤芹
Owner 广州百仕肽生物科技有限公司
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