Preparation method of lactic dehydrogenase determination reagent spheres, reagent spheres and microfluidic chip

Abstract

The embodiment of the invention relates to the technical field of medical detection, and particularly relates to a preparation method of lactic dehydrogenase determination reagent spheres, the reagent spheres and a microfluidic chip. The lactic dehydrogenase determination reagent spheres comprise a first reagent sphere and a second reagent sphere, wherein a first reagent solution forming the first reagent sphere comprises 40-200mmol / L of a first buffer solution, 0.1-10g / L of iodonitrochlorotetrazolium blue, 10-100g / L of a first excipient and 0.1-10 g / L of a stabilizer; and a second reagent solution forming the second reagent sphere comprises 40-200mmol / L of a second buffer solution, 10-100g / L of lactate, 10-50g / L of oxidized coenzyme I, 20-100U / L of diaphorase and 10-100g / L of a second excipient. During detection, the generated reduced coenzyme I (NADH) is further reacted to generate colored stable formazan, so that the concentration of lactic dehydrogenase can be quantified by detecting the absorbance at the wavelength of 490nm, and the sensitivity is greatly improved; and in addition, the first excipient and the second excipient in the dosage range can ensure that the first reagent sphere and the second reagent sphere have good morphology and re-melting solubility respectively, and have high stability and precision.

Description

Technical field [0001] Embodiments of the present invention relate to the field of medical detection, and more particularly to a method of preparing a lactate dehydrogenase measurement reagent ball, a reagent ball, a microfluidic chip. Background technique [0002] The lactate dehydrogenase is widely present in the body, the most in the liver, kidney, myocardium, skeletal muscle, pancreatic and pulmonary, and the concentration of lactate dehydrogenase in these tissues is much higher than the lactate dehydrogenase in serum. concentration. Therefore, when a small amount of tissue necrosis occurs, the lactate dehydrogenase in necrotic tissue releases blood to increase the concentration of degenerate dehydrogenase in the blood. Therefore, clinically, the concentration of lactate dehydrogenase is often used to assisted auxiliary diagnosis of myocardial infarction, liver disease and certain malignant tumors. [0003] At present, the concentration of lactate dehydrogenase in the serum i...

AI Technical Summary

Problems solved by technology

However, the activity of reduced coenzyme I (NADH) is easily interfered by various factors, and it is easily oxidized, which directly affects the measurement results of lactate dehydrogenase, resulting in low detection accuracy and low sensitivity.

In addition, it needs to rely on a large-scale automatic biochemical analyzer, which is difficult to apply to patients for rapid diagnosis, that is, POCT diagnosis

Although there are existing freeze-dried reagent balls used to detect other items, the shape of the existing freeze-dried reagent balls is poor, and it is difficult to completely freeze-dry, resulting in lower stability and accuracy.

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