Blood free DNA protection reagent, protection method and blood collection tube

A technology for protecting reagents and blood, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of reducing DNA polymerase activity, reducing the detection sensitivity of PCR reaction, and the influence of inspection and test results.

Pending Publication Date: 2021-08-17
SHENZHEN BIOCOMMA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to prolong the storage time of blood cell-free DNA, enzyme inhibitors are usually added to some storage reagents, but enzyme inhibitors will affect the subsequent inspection and detection of cell-free DNA
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Method used

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  • Blood free DNA protection reagent, protection method and blood collection tube
  • Blood free DNA protection reagent, protection method and blood collection tube
  • Blood free DNA protection reagent, protection method and blood collection tube

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Prepare the blood free DNA protection reagent, the protection reagent contains 70mg / ml tripotassium edetate hydrate, 400mg / ml imidazolidinyl urea, 10mg / ml polyvinyl alcohol, 40mg / ml trehalose and 30mg / ml ml of glycine, and store the prepared protection reagent in a vacuum blood collection tube. The volume ratio of protective reagent to blood is 1:7. Marked as group B1.

Embodiment 2

[0053] Prepare the blood free DNA protection reagent, the protection reagent contains 70mg / ml disodium edetate dihydrate, 400mg / ml diazolidinyl urea, 10mg / ml Tween-20, 40mg / ml polyethylene Alcohol and 30mg / ml Tris-HCL, and save the prepared protective reagent in a vacuum blood collection tube. The volume ratio of protective reagent to blood is 1:7. Marked as group B2.

Embodiment 3

[0055] Prepare the blood cell-free DNA protection reagent, the protection reagent contains 70mg / ml tripotassium edetate hydrate, 400mg / ml imidazolidinyl urea, 10mg / ml Tween-20, 40mg / ml trehalose and 30mg / ml of glycine, and save the prepared protection reagent in a vacuum blood collection tube. The volume ratio of protective reagent to blood is 1:10. Marked as group B3.

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PUM

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Abstract

The invention discloses a blood free DNA protection reagent, a protection method adopting the protection reagent and a blood collection tube containing the protection reagent. The protection reagent comprises 20-100 mg/ml of an anticoagulant, 100-600 mg/ml of a preservative, 10-50 mg/ml of an anti-adsorbent and 20-50 mg/ml of a buffering agent. The anticoagulant comprises at least one of tripotassium ethylenediaminetetraacetate hydrate, ethylenediaminetetraacetic acid and disodium ethylenediaminetetraacetate dihydrate, the preservative comprises at least one of imidazolidinyl urea, diazolidinyl urea and diazolidinyl urea, the anti-adsorbent comprises at least one of polyvinyl alcohol, trehalose and Tween-20, and the buffer agent comprises glycine or Tris-HCL. According to the blood free DNA protection reagent, the influence of the protection reagent on free DNA detection and detection results is reduced, and meanwhile, the blood free DNA protection reagent also has a good preservation effect on blood free DNA.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a blood free DNA protection reagent, protection method and blood collection tube. Background technique [0002] Blood free DNA (cell-free DNA, cfDNA) is a degraded DNA fragment released into plasma, which exists in various body fluids of the human body, and its concentration changes with the state of the human body. It is a new type of non-invasive molecular detection technology. have important clinical value. Blood cell-free DNA has important potential value for early diagnosis of diseases. The cell-free DNA of the fetus can dissociate into the blood of pregnant women. By detecting the cell-free DNA of the fetus in the blood of pregnant women, the male sex (Y chromosome) of the fetus can be predicted, and whether it has 21-trisomy, 18-trisomy, 12-trisomy Somatic and other chromosomal diseases. Cell-free DNA also has important potential value in the prognosis and monitoring of dis...

Claims

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Application Information

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IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2527/125
Inventor 雷秋亚陈高明胡玉梅
Owner SHENZHEN BIOCOMMA TECH
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