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Whitening composition, skin brightening lotion and preparation method thereof

A composition and whitening technology, applied in the field of daily chemicals, can solve the problems of single action of arbutin, skin irritation, easy discoloration of kojic acid, etc., and achieve the effect of inhibiting the formation of melanin, activating the function, and reducing the formation of melanin

Inactive Publication Date: 2021-08-10
GUANGZHOU KENENG COSMETICS RES CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, hydroquinone is very irritating to the skin; kojic acid is easy to change color and is also irritating to the skin; arbutin has a single effect and poor effect, etc.

Method used

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  • Whitening composition, skin brightening lotion and preparation method thereof
  • Whitening composition, skin brightening lotion and preparation method thereof
  • Whitening composition, skin brightening lotion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-6

[0071] The holly leaf and berberis extract was diluted with phosphate buffer to obtain 6 groups of test solutions with different concentrations (final concentrations), which were filtered with a 0.22 μm filter head. When performing cell viability test, the mass concentration of holly leaf and barberry extract is shown in Table 1 below.

[0072] Cell Viability Test

[0073] CCK-8 reagent contains WST-8: chemical name 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfone Acid phenyl)-2H-tetrazole monosodium salt, which is reduced to highly water-soluble Yellow formazan product, the amount of formazan produced is directly proportional to the number of viable cells. The light absorption value was measured at a wavelength of 450nm by an enzyme-linked immunosorbent assay instrument, which can indirectly reflect the number of living cells. This method has been widely used in the activity detection of some biologically active factors, large-scale antitumor drug screening...

Embodiment 7-12

[0081] The holly leaf and berberis extract was diluted with phosphate buffer to obtain 6 groups of test solutions with different concentrations (final concentrations), which were filtered with a 0.22 μm filter head. When performing the cell viability test, the mass concentration of the holly leaf and barberry extract is shown in Table 2 below.

[0082] Melanocyte norepinephrine receptor inhibition test

[0083]When norepinephrine activates melanocyte norepinephrine receptors, it generates cAMP via adenylyl cyclase (AC) activation. cAMP in turn activates PKA, which activates the gene expression of MITF through the phosphorylation of CREB. Finally, by interacting with TYR, TRP-1 and TRP-2 promoter regions, MITF gene is transcribed to produce melanin-related proteins. Therefore, detecting the concentration of intracellular cAMP can reflect the degree of activation of norepinephrine receptors.

[0084] Dilute melanoma B16 cells to 2.5*10 4 cells / ml, transfer the cell suspens...

Embodiment 13-17

[0093] The holly leaf and barberry extract, nicotinamide and bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylate were diluted with phosphate buffer to obtain 5 groups of test solutions with different concentrations (final concentrations). During the test, the mass concentrations of the holly leaf and barberry extract, nicotinamide and bis-diethoxydiethylene glycol cyclohexane 1,4-dicarboxylate are shown in Table 3 below.

[0094] Measurement of apparent brightness of 3D melanin skin model

[0095] The 3D melanin skin model is subjected to UVB irradiation, and the irradiation dose is 50mJ / cm each time 2 , continue for 7 days, after each irradiation, replace the 10*10 -9 mol / L norepinephrine culture medium. The blank group was not irradiated with UVB, and only the culture medium was changed every day. The surface administration of Examples 13-17 was carried out on the 6th and 8th days of the air-liquid level, and only the culture medium was replaced in the control g...

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Abstract

The invention belongs to the field of daily chemicals, and particularly provides a whitening composition, a skin brightening lotion and a preparation method of the skin brightening lotion. The composition for inhibiting melanogenesis provided by the invention is prepared from a berberis amurensis extract and nicotinamide. According to the composition for inhibiting melanogenesis, the berberis amurensis extract and the nicotinamide are matched, so the composition has a synergistic effect and can well inhibit melanogenesis. The skin brightening lotion prepared by using the composition for inhibiting melanogenesis can reduce the generation of melanin, has a whitening effect, can increase the moisture content of cuticle, and prevents moisture loss of deep skin.

Description

technical field [0001] The invention relates to the field of daily chemicals, in particular to a whitening composition, skin brightening water and a preparation method thereof. Background technique [0002] Melanin is mainly produced by melanocytes in the basal layer of human skin epidermis, which can reduce the damage of ultraviolet rays to the skin. The content and distribution of melanin determine the color of the skin. However, the abnormal accumulation of melanin in the basal layer will cause hyperpigmentation and cause melasma, freckles, age spots, etc., affecting people's quality of life. With the deepening of research on melanin biosynthesis, researchers found that tyrosinase plays an important role in melanin biosynthesis. It is the main rate-limiting enzyme in the process of converting tyrosine and dopa to melanin. Its overexpression It is an important cause of hyperpigmentation. Therefore, inhibiting the activity of tyrosinase can effectively block the biosynth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/67A61K8/34A61K8/49A61K8/55A61K8/60A61K8/63A61K8/64A61K8/65A61K8/68A61K8/9711A61K8/9722A61K8/9794A61Q19/02
CPCA61K8/9789A61K8/675A61K8/9794A61K8/553A61K8/678A61K8/68A61K8/9711A61K8/9722A61K8/65A61K8/4946A61K8/60A61K8/645A61K8/34A61K8/63A61Q19/02A61K2800/592A61K2800/84
Inventor 杨登亮林盛杰黄晓园刘露周雨叶邓慧张伟杰曾伟丹张楚标
Owner GUANGZHOU KENENG COSMETICS RES CO LTD
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