Prawn immunopotentiator based on RNA interference technology and preparation method and application of prawn immunopotentiator

Abstract

The invention discloses a prawn immunopotentiator based on an RNA interference technology and a preparation method and application of the prawn immunopotentiator. According to the prawn immunopotentiator, expression of LvFtz-F1 beta2 genes of litopenaeus vannamei is specifically interfered through dsRNA, a prawn JAK / STAT signal channel is activated, the immunity of prawns is enhanced, and the resistance of the prawns to pathogens is improved. After the prawn immunopotentiator is eaten by the prawns, the dsRNA is immediately absorbed by intestinal tracts along with digestion of feed, and the dsRNA entering the intestinal tracts plays a role in the intestinal tracts and is transported to other tissues and organs along with an open circulatory system of the prawns to finally cause systemic RNA interference. Moreover, the preparation cost is low, large-scale use in an industrial aquaculture system is easy, the specificity is high, the safety is high, and the prawn immunopotentiator has important significance in promoting the development of the prawn aquaculture industry.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a prawn immune enhancer based on RNA interference technology and its preparation method and application. Background technique [0002] Litopenaeus vannamei, commonly known as Penaeus vannamei, belongs to Arthropoda, Crustacea, Decapod, Penaeidae, Litopenaeus genus, because of its fast growth rate, high meat content, rich protein, disease resistance and stress resistance Strong and other characteristics, it is the main species of shrimp farming in the world. With the rise of the global economic level, people's living standards and consumption capacity are upgraded accordingly, and the market demand for prawns is increasing day by day. Fishing prawns from the wild can no longer satisfy the market's demand for healthy prawns. Driven by the huge market demand, the prawn farming industry has developed vigorously. However, with the expansion of the scale of shrimp farming and t...

AI Technical Summary

Problems solved by technology

Among them, for the chemical synthesis method, its synthesis cost is high, the efficiency is low, and it is difficult to meet the high-dose interference application; and the biosynthesis method can be divided into eukaryotic cell level synthesis and prokaryotic cell level synthesis, both of which are based on dsRNA or siRNA The expression vector expresses the interfering RNA required for the application in the host cell. The difference is that one of the host cells is a eukaryotic cell and the other is a bacterium. However, in related technologies, the biosynthesis method only stays at inhibiting insect At the level of growth and reproduction, the application in other more complex organisms has not been effectively developed

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