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Bifunctional soybean-derived polypeptide and preparation method thereof

A dual-function, dual-function technology, applied in the field of functional peptides, can solve the problem of less bifunctional peptides, and achieve the effects of long storage period, broad market application prospects, and simple and easy operation.

Active Publication Date: 2021-08-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current research on peptides focuses on the mining of single activity or drug application, and there are relatively few studies on bifunctional peptides and their application in the food industry.

Method used

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  • Bifunctional soybean-derived polypeptide and preparation method thereof
  • Bifunctional soybean-derived polypeptide and preparation method thereof
  • Bifunctional soybean-derived polypeptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: A method for preparing bifunctional black soybean polypeptide

[0067] This embodiment mainly includes the following steps:

[0068] (1) Extraction of isolated protein from soybean sources: crush and sieve black soybeans, add 3 times the volume of n-hexane to degrease; dissolve defatted black soybean powder in deionized water, adjust the pH of the solution to 9.0 with sodium hydroxide, stir at 30°C for 1.5 hours, and take it by centrifugation Then use hydrochloric acid to adjust the pH to 4.0, repeat the above steps twice, centrifuge at 10,000 rpm for 10 minutes to collect the protein precipitate, wash the precipitate with deionized water until neutral, and finally freeze-dry to obtain black soybean protein isolate.

[0069] (2) Infrared treatment: The protein obtained above was subjected to infrared heat treatment, the infrared temperature was 120° C., and the treatment time was 20 minutes.

[0070] (3) Polypeptide preparation: Dissolve infrared protein pow...

Embodiment 2

[0072] Example 2: A method for preparing bifunctional mung bean polypeptide

[0073] This embodiment mainly includes the following steps:

[0074] (1) Extraction of isolated protein from bean sources: crush and sieve mung beans, add 4 times the volume of n-hexane to degrease; dissolve defatted mung bean powder in deionized water, adjust the pH of the solution to 8.5 with sodium hydroxide, stir at room temperature for 2 hours, centrifuge and take the supernatant for reuse Adjust the pH to 4.5 with hydrochloric acid, repeat the above steps twice, centrifuge at 10,000 rpm for 10 minutes to collect the protein precipitate, wash the precipitate with deionized water until neutral, and finally freeze-dry to obtain mung bean protein isolate.

[0075] (2) Infrared treatment: The protein obtained above was subjected to infrared heat treatment, the infrared temperature was 100° C., and the treatment time was 25 minutes.

[0076] (3) Polypeptide preparation: Mung bean protein powder is d...

Embodiment 3

[0078] Example 3: A method for preparing bifunctional red bean polypeptide

[0079] This embodiment mainly includes the following steps:

[0080] (1) Extraction of isolated protein from bean source: crush and sieve red beans, add 4 times the volume of n-hexane to degrease; dissolve defatted red bean powder in deionized water, adjust the pH of the solution to 9.0 with sodium hydroxide, stir at room temperature for 2 hours, centrifuge and take the supernatant for reuse Adjust the pH to 4.3 with hydrochloric acid, repeat the above steps twice, centrifuge at 10,000 rpm for 10 minutes to collect the protein precipitate, wash the precipitate with deionized water until neutral, and finally freeze-dry to obtain the red bean protein isolate.

[0081] (2) Infrared treatment: The protein obtained above was subjected to infrared heat treatment, the infrared temperature was 100° C., and the treatment time was 20 minutes.

[0082] (3) Polypeptide preparation: red bean protein powder is dis...

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Abstract

The invention discloses a bifunctional soybean-derived polypeptide and a preparation method thereof, and belongs to the technical field of functional polypeptides. The method comprises the following steps: firstly, extracting bean-source isolated protein from beans by adopting an alkali-solution and acid-isolation method, then carrying out infrared heat treatment on the bean-source isolated protein, and finally, carrying out protease enzymolysis to obtain the bean-source polypeptide. The bean-source polypeptide has dual functions of antihypertensive activity and antioxidant activity at the same time.

Description

technical field [0001] The invention relates to a bifunctional bean-derived polypeptide and a preparation method thereof, belonging to the technical field of functional polypeptides. Background technique [0002] With people's pursuit of nutrition, health and sustainable development, plant-based foods are becoming more and more popular. As an indispensable raw material for plant-based foods, the demand for plant protein and its derivatives also shows a continuous growth trend. Beans are the main source of plant protein, and their protein content is about 20-40%, which is 1-3 times higher than cereals and 3-5 times higher than potatoes. However, the complex and dense structure of natural soybean protein greatly limits its application in the food industry. Therefore, it is very important to find a suitable method to modify soybean protein to improve its nutritional and functional properties. [0003] Due to its safety and effectiveness, enzymatic modification is widely used...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18A23L29/00A23L3/3472A61K8/64A61Q19/08C12P21/06C07K1/30C07K1/14B01J13/02
CPCA23L33/18A23L29/04A23L3/3472A61K8/645A61Q19/08C12P21/06C07K1/30C07K1/145B01J13/02A23V2002/00A23V2200/326A23V2250/55A23V2250/18A23V2200/02A23V2250/21A23D7/0053A23J3/14A23J1/14C07K14/415A23J3/346A23D7/04A61K38/168A61Q19/00A23J3/16A61K2800/10
Inventor 刘元法郑召君陈音徐勇将杜彦鹏
Owner JIANGNAN UNIV
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