Quantitative detection method for <14>C in urine

A quantitative detection method, urine technology, applied in the field of radiation health, can solve problems such as insufficient analytical methods, achieve good recovery rate and accuracy, and remove interference

Pending Publication Date: 2021-07-30
国家卫生健康委职业安全卫生研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] for existing 14 The problem that C analysis method is not sound enough, the purpose of the present invention is to provide a kind of urine 14 The detection method of C to realize the occupational health monitoring of the staff in relevant places

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  • Quantitative detection method for &lt;14&gt;C in urine
  • Quantitative detection method for &lt;14&gt;C in urine
  • Quantitative detection method for &lt;14&gt;C in urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The present invention will be described in further detail below in conjunction with accompanying drawing and specific embodiment, embodiment 1: adopt urea carrier to optimize

[0044] (1) Weigh 0.7g, 0.9g, 1.1g, 1.3g, 1.5g of urea in turn, add 50mL of water to obtain the tested sample;

[0045](2) Put the sample into a three-necked flask, add 10g of potassium persulfate, connect the condensing device, feed nitrogen, control the gas flow rate to 0.5L / min, and connect two gas outlets with 50mL 1mol / L sodium hydroxide absorption liquid;

[0046] (3) Adjust the temperature to 95±3°C, turn on the magnetic stirring, start timing when the temperature rises to 95°C, and the reaction time is 60 minutes;

[0047] (4) After the reaction is over, transfer the absorption solution to a beaker, and adjust the pH to 10.0-10.5 with ammonium chloride;

[0048] (5) Add saturated calcium chloride solution to form a precipitate, and let it stand until the supernatant is clarified;

[0049...

Embodiment 2

[0054] Embodiment 2: reaction time optimization

[0055] (1) Take several portions of 0.7g urea, add 50mL water, and obtain the tested sample;

[0056] (2) Put the sample into a three-necked flask, add 10g of potassium persulfate, connect the condensing device, feed nitrogen, control the nitrogen flow rate to 0.5L / min, and connect two gas outlets with 50 ml of 1mol / L sodium hydroxide absorption solution ;

[0057] (3) Set the temperature to 95±3°C, turn on the magnetic stirring, start timing when the temperature rises to 95°C, and the reaction time is 90min, 80min, 70min, 60min, 50min, 40min, 30min, 20min;

[0058] (4) After the reaction is over, transfer the absorption solution to a beaker, and adjust the pH to 10.0-10.5 with ammonium chloride;

[0059] (5) Add saturated calcium chloride solution to form a precipitate, and let it stand until the supernatant is clarified;

[0060] (6) Suction filter the precipitate, and add ethanol solution to make the precipitate dry quick...

Embodiment 3

[0065] Embodiment 3: Urine sample determination

[0066] (1) Take 120mL urine sample to obtain the tested sample;

[0067] (2) Put the sample into a three-necked flask, add 10g of potassium persulfate, connect the condensing device, feed nitrogen, control the nitrogen flow rate to 0.5L / min, and connect two gas outlets with 50 mL of 1mol / L sodium hydroxide absorption solution ;

[0068] (3) Adjust the temperature to 95±3°C, turn on the magnetic stirring, start timing when the temperature rises to 95°C, and the reaction time is 60 minutes;

[0069] (4) After the reaction is over, transfer the absorption solution to a beaker, and adjust the pH to 10.0-10.5 with ammonium chloride;

[0070] (5) Add saturated calcium chloride solution to form a precipitate, and let it stand until the supernatant is clarified;

[0071] (6) Suction filter the precipitate, and add ethanol solution to make the precipitate dry quickly, and then put it into an oven for drying;

[0072] (7) After dryin...

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Abstract

The invention discloses a quantitative detection method for <14>C in urine, which belongs to the technical field of radiohygiene. The method comprises the following steps of (1) measuring a certain volume of urine sample, adding potassium persulfate, heating, stirring, converting organic carbon and inorganic carbon in the urine sample into carbon dioxide, purging the generated carbon dioxide through carrier gas, and absorbing with alkali liquor, and (2) adjusting the pH value of the absorption liquid with ammonium chloride, adding a saturated calcium chloride solution to generate calcium carbonate precipitate, standing, carrying out suction filtration, and drying, and (3) grinding the prepared calcium carbonate precipitate powder into powder by using a mortar, weighing a proper amount of powder, and carrying out <14>C activity measurement by using a liquid scintillation counter. The method provided by the invention has the advantages of high accuracy, high recovery rate and stable detection result, and can meet the analysis of the activity concentration of <14>C in the urine sample.

Description

technical field [0001] The invention belongs to the technical field of radiation sanitation, and relates to a 14 C detection method. Background technique [0002] 14 C is a weak β radionuclide with a maximum energy of 156keV and a half-life of 5730 years. Its sources are natural and artificial. natural 14 The main source of C is cosmic rays and 14 (n,p) response of N; artificial 14 The sources of C are mainly nuclear explosion experiments and nuclear fuel cycle (nuclear power reactors and spent fuel reprocessing plants), which are mainly discharged into the environment through gaseous effluents and liquid effluents. in the air 14 C can participate in the photosynthesis of plants and thus enter the biosphere. Since carbon is an important element that composes organisms, therefore 14 After C is absorbed by the human body, there may be long-term exposure risks. Organizations such as the World Health Organization and the United Nations Environment Program 14 C is defined...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N23/00
CPCG01N1/28G01N23/00
Inventor 陈飞熊强闫琳琳曹静李玉文梁婧张震张建芳樊晶光
Owner 国家卫生健康委职业安全卫生研究中心
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