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Gamma-decalactone prepared based on biotransformation and preparation method thereof

A technology of biotransformation and decanolactone, which is applied in the field of spice processing, can solve the problems of yeast cell toxicity, low yield of γ-decalactone, long conversion cycle, etc., and achieve the effects of improving activity, realizing waste utilization, and rich sources

Pending Publication Date: 2021-07-30
ANHUI HYEA AROMAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of gamma-decalactone prepared based on biotransformation and its preparation method, to solve the relatively single substrate in the existing biotransformation technology, the toxicity of ricinoleic acid to yeast cells, and the toxicity of ricinoleic acid to yeast cells. The utilization rate of γ-decalactone is low, the conversion period is long, the output of γ-decalactone is low, and the production cost is high; and the production of γ-decalactone by using oleic acid as a substrate by biofermentation and conversion technology to improve the production of γ-decalactone Yield of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of γ-decalactone prepared based on biotransformation is inoculated into the fermentation medium by Yarrowia lipolytica, and the obtained fermentation liquid is transformed and inoculated into the transformation medium for transformation culture, adding batches during the process The waste oil mixture and oleic acid hydratase are obtained by continuing the conversion culture, and specifically include the following steps:

[0029] Step 1: Inoculate the Yarrowia lipolytica suspension into the fermentation medium, cultivate at 35°C, cultivate for 12h, and obtain the fermentation broth, wherein the Yarrowia lipolytica suspension has an OD600 value of 0.1, and the fermentation medium is It is prepared from glucose, yeast extract, ammonium dihydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate, Tween 80 and deionized water, and the pH of the resulting solution is adjusted to 7.5, and then sterilized at 110°C for 30 minutes, wherein , the mass ratio of g...

Embodiment 2

[0035] A kind of γ-decalactone prepared based on biotransformation is inoculated into the fermentation medium by Yarrowia lipolytica, and the obtained fermentation liquid is transformed and inoculated into the transformation medium for transformation culture, adding batches during the process The waste oil mixture and oleic acid hydratase are obtained by continuing transformation culture, and specifically include the following steps:

[0036] Step 1: Inoculate the Yarrowia lipolytica suspension into the fermentation medium, cultivate at 35°C, cultivate for 12h, and obtain the fermentation broth, wherein the Yarrowia lipolytica suspension has an OD600 value of 0.1, and the fermentation medium is It is prepared from glucose, yeast extract, ammonium dihydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate, Tween 80 and deionized water, and the pH of the resulting solution is adjusted to 7.5, and then sterilized at 110°C for 30 minutes, wherein , the mass ratio of g...

Embodiment 3

[0042] A kind of γ-decalactone prepared based on biotransformation is inoculated into the fermentation medium by Yarrowia lipolytica, and the obtained fermentation liquid is transformed and inoculated into the transformation medium for transformation culture, adding batches during the process The waste oil mixture and oleic acid hydratase are obtained by continuing transformation culture, and specifically include the following steps:

[0043] Step 1: Inoculate the Yarrowia lipolytica suspension into the fermentation medium, cultivate at 35°C, cultivate for 12h, and obtain the fermentation broth, wherein the Yarrowia lipolytica suspension has an OD600 value of 0.1, and the fermentation medium is It is prepared from glucose, yeast extract, ammonium dihydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate, Tween 80 and deionized water, and the pH of the resulting solution is adjusted to 7.5, and then sterilized at 110°C for 30 minutes, wherein , the mass ratio of g...

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Abstract

The invention relates to gamma-decalactone prepared based on biotransformation and a preparation method thereof. The gamma-decalactone is prepared by the following steps: inoculating yarrowia lipolytica into a fermentation culture medium, transforming and inoculating the obtained fermentation liquor into a transformation culture medium, carrying out transformation culture, adding oleic acid and oleic acid hydratase in batches in the process, and continuously carrying out transformation culture. The gamma-decalactone is produced through a biological fermentation and conversion technology with the waste grease mixture as the substrate, the problem that in an existing biological conversion technology, the substrate is single is solved with the waste grease mixture as the substrate, the waste grease mixture is rich in source, waste utilization is achieved, and the method is economical and environmentally friendly; meanwhile, the lactococcus gasseri genome DNA is used as a template, the oleic acid hydratase is obtained through PCR amplification, the activity of the oleic acid hydratase is improved, and the production yield of the gamma-decalactone is increased.

Description

technical field [0001] The invention belongs to the technical field of spice processing, and in particular relates to γ-decalactone prepared based on biotransformation and a preparation method thereof. Background technique [0002] γ-decalactone (γ-decalactone, GDL) is a lactone flavor naturally present in strawberries, peaches and other fruits. It has a low aroma threshold and is internationally recognized as a safe food additive. Coconut, strawberry, peach and other spices are widely used in margarine, ice cream, soft drinks, candy, baked goods and seasonings, and the market demand is very large. With the increasing consumption of delicious food, beverages, etc., the demand for natural food flavoring additives is increasing. [0003] At present, there are three ways to obtain γ-decalactone: natural extraction, chemical synthesis and biological preparation. γ-decalactone naturally exists in peaches, almonds, strawberries and other fruits, so γ-decalactone can be extracted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/04C12N9/88C12N15/60C12N15/10C12R1/645
CPCC12P17/04C12N9/88C12Y402/01053
Inventor 王天义范一义汪洋张政吴旭何云飞汪炎
Owner ANHUI HYEA AROMAS
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